Nanotechnology Now

Our NanoNews Digest Sponsors
Heifer International



Home > Press > Plasmonic biosensors with artificial antibodies

a) TEM image of gold nanorods; b) electric field distribution at the extinction maximum of the longitudinal SPR band; and c) illustration of imprinted nanorod with siloxane copolymer preferentially grown at the ends.
a) TEM image of gold nanorods; b) electric field distribution at the extinction maximum of the longitudinal SPR band; and c) illustration of imprinted nanorod with siloxane copolymer preferentially grown at the ends.

Abstract:
The use of localized surface plasmon resonance (SPR) as a platform in biological sensing holds immense promise in delivering devices with superior sensitivity to those of competing technologies. Until now, a major drawback has been that the production of plasmonic biosensors required the use of natural antibodies, making the process expensive and time-consuming. An important step toward overcoming this problem has recently been made, with researchers at Washington University reporting a novel plasmonic biosensor with ‘artificial' antibodies.

Plasmonic biosensors with artificial antibodies

Germany | Posted on November 7th, 2012

The biosensor developed is based on very small changes in the SPR spectrum of gold nanorods upon selective binding of a target biomolecule (binding changes the refractive index surrounding the nanorod and thereby shifts the plasmon band). In order to achieve high selectivity, the researchers used the method of macromolecular imprinting to incorporate target species—as a proof-of-concept, several different proteins were used—into a polymer network in close vicinity (several nanometers) to the nanorod. Removal of the target leaves behind a cavity in the polymer, which acts as a ‘lock and key' receptor enabling selective binding of the analyte.

In order to attain maximal sensitivity, it is imperative that the polymer and the molecularly imprinted species are concentrated at the ends of the nanorod. This requirement owes to the fact that the electric field surrounding the nanorod is nonuniform, with the longitudinal resonance being stronger and more sensitive than the transverse (the electric field distribution of the longitudinal resonance is shown in figure panel (b)). This was achieved with the help of a surfactant (CTAB), which is known to adsorb preferentially at the sides of the nanorod. Specific chemicals employed to form a bridge between the nanorod and the polymer precursors were then able to interact preferentially with thiol groups on the ends of the nanorod due to the lower surfactant concentration. The result after polymerisation: a peanut shaped polymer-nanorod with the majority of the polymer and reversible template inclusions (i.e., artificial antibodies) at the ends where the SPR is most sensitivly affected by analyte binding (see figure panel (c)).

To demonstrate the viability of their biosensor in a real-world system, the researchers successfully detected recombinant human neutrophil gelatinase-associated lipocalin (NGAL), a urinary biomarker of acute kidney injury, with detection limits lower than those of conventional analytical techniques. Although emulating the performance of natural antibodies does remain a challenge, the authors suggest ways this could be achieved, meaning we are likely to hear a lot more about these novel biosensors in the near future.

####

For more information, please click here

Copyright © Wiley-VCH Materials Science Journals

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

Link to the original paper on Wiley Online Library:

Related News Press

News and information

Virginia Tech physicists propose path to faster, more flexible robots: Virginia Tech physicists revealed a microscopic phenomenon that could greatly improve the performance of soft devices, such as agile flexible robots or microscopic capsules for drug delivery May 17th, 2024

Gene therapy relieves back pain, repairs damaged disc in mice: Study suggests nanocarriers loaded with DNA could replace opioids May 17th, 2024

Shedding light on perovskite hydrides using a new deposition technique: Researchers develop a methodology to grow single-crystal perovskite hydrides, enabling accurate hydride conductivity measurements May 17th, 2024

Oscillating paramagnetic Meissner effect and Berezinskii-Kosterlitz-Thouless transition in cuprate superconductor May 17th, 2024

Nanomedicine

Virginia Tech physicists propose path to faster, more flexible robots: Virginia Tech physicists revealed a microscopic phenomenon that could greatly improve the performance of soft devices, such as agile flexible robots or microscopic capsules for drug delivery May 17th, 2024

Diamond glitter: A play of colors with artificial DNA crystals May 17th, 2024

Advances in priming B cell immunity against HIV pave the way to future HIV vaccines, shows quartet of new studies May 17th, 2024

New micromaterial releases nanoparticles that selectively destroy cancer cells April 5th, 2024

Sensors

Innovative sensing platform unlocks ultrahigh sensitivity in conventional sensors: Lan Yang and her team have developed new plug-and-play hardware to dramatically enhance the sensitivity of optical sensors April 5th, 2024

$900,000 awarded to optimize graphene energy harvesting devices: The WoodNext Foundation's commitment to U of A physicist Paul Thibado will be used to develop sensor systems compatible with six different power sources January 12th, 2024

A color-based sensor to emulate skin's sensitivity: In a step toward more autonomous soft robots and wearable technologies, EPFL researchers have created a device that uses color to simultaneously sense multiple mechanical and temperature stimuli December 8th, 2023

New tools will help study quantum chemistry aboard the International Space Station: Rochester Professor Nicholas Bigelow helped develop experiments conducted at NASA’s Cold Atom Lab to probe the fundamental nature of the world around us November 17th, 2023

Discoveries

Virginia Tech physicists propose path to faster, more flexible robots: Virginia Tech physicists revealed a microscopic phenomenon that could greatly improve the performance of soft devices, such as agile flexible robots or microscopic capsules for drug delivery May 17th, 2024

Diamond glitter: A play of colors with artificial DNA crystals May 17th, 2024

Finding quantum order in chaos May 17th, 2024

Advances in priming B cell immunity against HIV pave the way to future HIV vaccines, shows quartet of new studies May 17th, 2024

Announcements

Virginia Tech physicists propose path to faster, more flexible robots: Virginia Tech physicists revealed a microscopic phenomenon that could greatly improve the performance of soft devices, such as agile flexible robots or microscopic capsules for drug delivery May 17th, 2024

Diamond glitter: A play of colors with artificial DNA crystals May 17th, 2024

Finding quantum order in chaos May 17th, 2024

Oscillating paramagnetic Meissner effect and Berezinskii-Kosterlitz-Thouless transition in cuprate superconductor May 17th, 2024

Nanobiotechnology

Diamond glitter: A play of colors with artificial DNA crystals May 17th, 2024

Advances in priming B cell immunity against HIV pave the way to future HIV vaccines, shows quartet of new studies May 17th, 2024

New micromaterial releases nanoparticles that selectively destroy cancer cells April 5th, 2024

Good as gold - improving infectious disease testing with gold nanoparticles April 5th, 2024

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project