Home > Press > Engineering a new single-walled carbon nanotubes- molecular beacon-daunorubicin sensor for detection of Salmonella typhimurium carrying SSeC gene
Abstract:
A novel single-walled carbon nanotubes(SWNTs)-molecular beacons(MBs) -daunoru -bicin(DNR) conjugate was devised as a targeted sensing system to detect S. typhimurium. The system, signified as SWNTs-MB(DNR), was fabricated by covalently coupling of a MB included complementary sequence targeted to the SSeC of the bacteria through EDC/Sulfo-NHS chemistry and insetting daunorubicin to the stem region of the molecular beacon on carbon nanotubes before adding the target sequence.
The fluorescence of daunorubicin, originally, was fairly week because of dual fluorescence quenching. When mixing target sequence with SWNTs-MBs(DNR), it was seen that the daunorubicin was discharged from hairpin stem due to the formation of rigid structure between the loop of the MB and the target sequence, which resulted in increase of fluorescence intensity substantially and attained to target quantitation. On the contrary, mismatch oligonucleotides of other Salmonella sps. with different sero types did not enhance the fluorescence intensity considerably, which represented a high selectivity and specificity of the biosensor. The experimental results demonstrated patently that the recovery of fluorescence of daunorubicin is proportion to the concentration of the target DNA with the range 0.2-0.7́M and the low detection limit is 50nM. Consequently, the SWNTs-MBs(DNR) architecture can be an excellent platform to take place of the common molecular beacon easily and has the potential to study MB based on aptamer binding to the SWNTs.
####
For more information, please click here
Contacts:
Le Deng
Phone: +86 0731 88872927
Fax: +86 0731 88883310
Copyright © The Co-construction Laboratory of Microb
If you have a comment, please Contact us.Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.
Related News Press |
News and information
New organic molecule shatters phosphorescence efficiency records and paves way for rare metal-free applications July 5th, 2024
Single atoms show their true color July 5th, 2024
New method cracked for high-capacity, secure quantum communication July 5th, 2024
Searching for dark matter with the coldest quantum detectors in the world July 5th, 2024
Nanotubes/Buckyballs/Fullerenes/Nanorods/Nanostrings
Tests find no free-standing nanotubes released from tire tread wear September 8th, 2023
Detection of bacteria and viruses with fluorescent nanotubes July 21st, 2023
Nanomedicine
The mechanism of a novel circular RNA circZFR that promotes colorectal cancer progression July 5th, 2024
Diamond glitter: A play of colors with artificial DNA crystals May 17th, 2024
Advances in priming B cell immunity against HIV pave the way to future HIV vaccines, shows quartet of new studies May 17th, 2024
Sensors
Discoveries
Efficient and stable hybrid perovskite-organic light-emitting diodes with external quantum efficiency exceeding 40 per cent July 5th, 2024
A New Blue: Mysterious origin of the ribbontail ray’s electric blue spots revealed July 5th, 2024
New organic molecule shatters phosphorescence efficiency records and paves way for rare metal-free applications July 5th, 2024
Single atoms show their true color July 5th, 2024
Announcements
New organic molecule shatters phosphorescence efficiency records and paves way for rare metal-free applications July 5th, 2024
Single atoms show their true color July 5th, 2024
New method cracked for high-capacity, secure quantum communication July 5th, 2024
Searching for dark matter with the coldest quantum detectors in the world July 5th, 2024
Patents/IP/Tech Transfer/Licensing
Getting drugs across the blood-brain barrier using nanoparticles March 3rd, 2023
Metasurfaces control polarized light at will: New research unlocks the hidden potential of metasurfaces August 13th, 2021
Arrowhead Pharmaceuticals Announces Closing of Agreement with Takeda November 27th, 2020
Food/Agriculture/Supplements
Silver nanoparticles: guaranteeing antimicrobial safe-tea November 17th, 2023
Night-time radiative warming using the atmosphere November 17th, 2023
DGIST and New Life Group launched a research project on "Functional beauty and health products using the latest nanotechnology" May 12th, 2023
![]() |
||
![]() |
||
The latest news from around the world, FREE | ||
![]() |
![]() |
||
Premium Products | ||
![]() |
||
Only the news you want to read!
Learn More |
||
![]() |
||
Full-service, expert consulting
Learn More |
||
![]() |