Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Microchip proves tightness provokes precocious sperm release

Sperm cell release can be triggered by tightening the grip around the delivery organ, according to a team of nano and microsystems engineers and plant biologists at the University of Montreal and Concordia University. Concordia's nanobiotech team devised a microchip that enabled the University of Montreal biologists to observe what happened when pollen tubes -- the sperm delivery tools used by plants -- tried to negotiate a microscopic obstacle course.

Credit: Universite de Montreal and Concordia University
Sperm cell release can be triggered by tightening the grip around the delivery organ, according to a team of nano and microsystems engineers and plant biologists at the University of Montreal and Concordia University. Concordia's nanobiotech team devised a microchip that enabled the University of Montreal biologists to observe what happened when pollen tubes -- the sperm delivery tools used by plants -- tried to negotiate a microscopic obstacle course.

Credit: Universite de Montreal and Concordia University

Abstract:
Sperm cell release can be triggered by tightening the grip around the delivery organ, according to a team of nano and microsystems engineers and plant biologists at the University of Montreal and Concordia University. Concordia's nanobiotech team devised a microchip that enabled the University of Montreal biologists to observe what happened when pollen tubes - the sperm delivery tools used by plants - tried to negotiate a microscopic obstacle course. The pollen tubes were exposed to a series of narrow, elastic openings resulting in a variety of cellular responses. When the opening was too narrow or tight, pollen tube growth stalled. However, the elongating tubes successfully penetrated slightly larger openings. Curiously, the pollen tubes burst and released the sperm cells when passing openings of a particular size relative to the pollen tube width.

Microchip proves tightness provokes precocious sperm release

Montreal, Canada | Posted on April 29th, 2013

The microchip was designed to imitate the mechanical challenges that the female flower tissues place in the path of the rapidly growing pollen tube on its way to the egg cell. Unlike its human counterpart, a microscopic single-cell organ undertakes sperm delivery in plants: a cylindrical protuberance formed by the male gametophyte, the pollen grain. "Similarly to elongated human cells such as neurons, the pollen tubes are tip growing cells that invade other tissues, in this case those of the female flower organs. Unlike those found in humans or other animals, the invasive ability of tip growing cells in plants remain largely unexplored. Our goal was to address this lack of knowledge using pollen tubes, whose invasive life style is the fundamental underpinning of sexual reproduction in flowering plants," said senior co-author Anja Geitmann of the University of Montreal. "Since they are encased in a stiff cellular envelope, plant cells grow and invade differently from animal cells," explained Concordia University senior co-author Muthukumaran Packirisamy. "From a mechanical point of view, the process of pollen tube elongation is similar to that of a balloon catheter used for angioplasty - forces are generated based on the principle of a hydroskeleton, or fluid under pressure. We designed microchannels through which the pollen tubes had to forcefully squeeze in order to continue their elongation."

Microsystems technology was required to undertake this experiment due to the tiny size of pollen tubes and the consequently minute amount of force they exert to accomplish penetration. The pollen tube requires not only an invasive force to overcome the mechanical resistance of the surrounding tissue, but also the protection of the contents that is to be transported, the sperm cells, of which there are exactly two in each tube. It is crucial that the pollen tube remains tubular while winding its way through the pistil, since kinks and collapses of this catheter-like organ prevent the passage of the sperm cells, similar to a blockage in a twisted garden hose. A typical pollen tube can become many centimeters long but is only between 5 and 20 micrometres wide. By way of comparison, a human hair is typically 100 micrometres thick. "To fit the dimensions of this cell, we had to design the microchip with microscopic channels and obstacles that are narrower than the pollen tubes. We used high resolution soft and direct write lithography techniques to produce this micron-sized obstacle course. Furthermore, measuring the pressure that pollen tubes exert requires materials that are just the right consistency - not too hard and not too soft. We chose an elastic polymer material, and as the tubes deformed the material at some levels of tightness, we can be certain that the softness was just right. Mechanical modeling allowed us to calculate the forces exerted by these cells. Lab-on-a-Chip (LOC) technology allows us to assess cellular behavior much better than the conventional Petri dish," Packirisamy said.

Sexual reproduction in plants is in many ways analogous to human biology. "In order to find its path to the ovule and the egg cell, the pollen tube has to invade a series of female tissues in the receptive flower," Geitmann explained. "The male and the female organs continuously communicate during the process. The female aids the process by lubricating the path, but the male exerts significant force to overcome any residual resistance." The pollen tube must penetrate through a central canal that connects the stigma, or pollen landing platform, to the ovary, the organ that houses the ovules. Upon reaching the ovary, it then passes onto the internal surface of the placenta, finds and enters the opening in the ovule, and finally bursts open when connecting with the egg cell, enabling its fertilization by one of the two sperm cells it delivers. The other sperm cell fertilizes another cell of the female gametophyte to give rise to a tissue that nourishes the growing embryo.

How the timely discharge of the sperm cells is triggered in plants has essentially been unknown. The control of this process is crucial for successful fertilization and seed set, since plant sperm is not motile and must therefore be delivered precisely to its target. A precocious discharge (before the pollen tube reaches the ovule) will not allow the sperm cells to reach the egg, whereas on the other hand, a failure to discharge equally precludes fertilization. "Our findings show that a tight grip around the tube does result in sperm release. This illustrates that plant cells perceive and respond to mechanical stimuli," Geitmann said. "However, the particularities of the cellular responses we observed suggest that other factors, such as protein-based signals, are likely to be in play." The researchers believe that these particularities and the mechanisms that control pollen tube diameter and growth behavior warrant further research. "We're still not sure exactly what causes the sperm cell release from the pollen tube in vivo, but a mechanical trigger might be part of the signaling mechanism," Packirisamy said.

###

About this study:

Amir Sanati Nezhad, Mahsa Naghavi, Muthukumaran Packirisamy, Rama Bhat and Anja Geitmann published "Quantification of cellular penetrative forces using Lab-on-a-Chip technology and finite element modeling" in the Proceedings of the National Academy of Sciences. This study received funding from Fonds de recherche du Québec - Nature et Technologies. Mechanical modeling expertise was contributed by co-author Professor Rama Bhat, Concordia University. The University of Montreal is officially known as Université de Montréal.

####

For more information, please click here

Contacts:
William Raillant-Clark

514-566-3813

Copyright © University of Montreal

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Transparent, electrically conductive network of encapsulated silver nanowires: A novel electrode for optoelectronics August 1st, 2015

Harris & Harris Group Portfolio Company, HZO, Announces Partnerships with Dell and Motorola August 1st, 2015

Advances and Applications in Biosensing, Sensor Power, and Sensor R&D to be Covered at Sensors Global Summit August 1st, 2015

Kalam: versatility personified August 1st, 2015

Imaging

Take a trip through the brain July 30th, 2015

Publication on Atomic Force Microscopy based nanoscale IR Spectroscopy (AFM-IR) persists as a 2015 top downloaded paper July 29th, 2015

Short wavelength plasmons observed in nanotubes: Berkeley Lab researchers create Ludinger liquid plasmons in metallic SWNTs July 28th, 2015

Reshaping the solar spectrum to turn light to electricity: UC Riverside researchers find a way to use the infrared region of the sun's spectrum to make solar cells more efficient July 27th, 2015

Superfast fluorescence sets new speed record: Plasmonic device has speed and efficiency to serve optical computers July 27th, 2015

Lab-on-a-chip

Smart hydrogel coating creates 'stick-slip' control of capillary action July 27th, 2015

Miniature Technology, Large-Scale Impact: Winner of the 2015 Lindros Award for translational medicine, Kjeld Janssen is pushing the boundaries of the emerging lab-on-a-chip technology July 7th, 2015

Discoveries

Gold-diamond nanodevice for hyperlocalised cancer therapy: Gold nanorods can be used as remote controlled nanoheaters delivering the right amount of thermal treatment to cancer cells, thanks to diamond nanocrystals used as temperature sensors August 1st, 2015

Shaping the hilly landscapes of a semi-conductor nanoworld August 1st, 2015

Solid state physics: Quantum matter stuck in unrest August 1st, 2015

Self-assembling, biomimetic membranes may aid water filtration August 1st, 2015

Announcements

Self-assembling, biomimetic membranes may aid water filtration August 1st, 2015

Transparent, electrically conductive network of encapsulated silver nanowires: A novel electrode for optoelectronics August 1st, 2015

Harris & Harris Group Portfolio Company, HZO, Announces Partnerships with Dell and Motorola August 1st, 2015

Advances and Applications in Biosensing, Sensor Power, and Sensor R&D to be Covered at Sensors Global Summit August 1st, 2015

Tools

Heating and cooling with light leads to ultrafast DNA diagnostics July 31st, 2015

Take a trip through the brain July 30th, 2015

Publication on Atomic Force Microscopy based nanoscale IR Spectroscopy (AFM-IR) persists as a 2015 top downloaded paper July 29th, 2015

Nanometrics Announces Upcoming Investor Events July 28th, 2015

Food/Agriculture/Supplements

Heating and cooling with light leads to ultrafast DNA diagnostics July 31st, 2015

Detecting small metallic contaminants in food via magnetization: A practical metallic-contaminant detecting system using three high-Tc RF superconducting quantum interference devices (SQUIDs) July 29th, 2015

QuantumSphere Completes State-of-the-Art Nanocatalyst Production Facility: Now Positioned to Capitalize on Commercial Validation and JDA with Casale, SA July 25th, 2015

3D-printed 'smart cap' uses electronics to sense spoiled food July 20th, 2015

Nanobiotechnology

Heating and cooling with light leads to ultrafast DNA diagnostics July 31st, 2015

European Technology Platform for Nanomedicine and ENATRANS European Consortium Launch the 2nd edition of the Nanomedicine Award: The Award to be presented at BIO-Europe conference in Munich, November 2015 July 30th, 2015

New computer model could explain how simple molecules took first step toward life: Two Brookhaven researchers developed theoretical model to explain the origins of self-replicating molecules July 28th, 2015

Spintronics: Molecules stabilizing magnetism: Organic molecules fixing the magnetic orientation of a cobalt surface/ building block for a compact and low-cost storage technology/ publication in Nature Materials July 25th, 2015

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project