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PCR products of methylenetetrahydrofolate reductase C677T DNA samples extracted with magnetic nanoparticles using saliva samples (lanes 1𔃃) and with traditional methods using blood samples (lanes 7󈝷).

Credit: Neural Regeneration Research

Abstract:
C677T polymorphism in the methylenetetrahydrofolate reductase (MTHFR) gene is a risk factor for stroke. Studies have report a higher C677T homozygosity frequency in Chinese than in Europeans.

A magnetic nanoparticles-based method for DNA extraction from the saliva after stroke

Shenyang, PR China | Posted on December 24th, 2013

Usually, the detection of genetic diseases is rather complicated on a large scale owing to the invasive sampling and a cumbersome DNA extraction process. In a study by Dr. Li Yi and team from Peking University Shenzhen Hospital in China, DNA from stroke patients and healthy controls was extracted from the saliva using a magnetic nanoparticles-based method and from the blood using conventional methods. Real-time PCR results revealed that the C677T polymorphism was genotyped by PCR using DNA extracted from both saliva and blood samples. The genotype results were confirmed by gene sequencing, and results for saliva and blood samples were consistent. Therefore, this noninvasive magnetic nanoparticles-based method using saliva samples could be used to screen for the MTHFR C677T polymorphism in target populations. This study was published in the Neural Regeneration Research (Vol. 8, No. 32, 2013).

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Contacts:
Meng Zhao

86-138-049-98773
Neural Regeneration Research

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