Nanotechnology Now

Our NanoNews Digest Sponsors


Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > MU Researchers Develop Advanced Three-Dimensional “Force Microscope”: Innovation could lead to faster drug therapies and increased understanding of proteins on the microscopic level

King and fellow researchers developed a three-dimensional microscope that will yield unparalleled study of membrane proteins and how they interact on the cellular level.
King and fellow researchers developed a three-dimensional microscope that will yield unparalleled study of membrane proteins and how they interact on the cellular level.

Abstract:
Membrane proteins are the "gatekeepers" that allow information and molecules to pass into and out of a cell. Until recently, the microscopic study of these complex proteins has been restricted due to limitations of "force microscopes" that are available to researchers and the one-dimensional results these microscopes reveal. Now, researchers at the University of Missouri have developed a three-dimensional microscope that will yield unparalleled study of membrane proteins and how they interact on the cellular level. These microscopes could help pharmaceutical companies bring drugs to market faster.

MU Researchers Develop Advanced Three-Dimensional “Force Microscope”: Innovation could lead to faster drug therapies and increased understanding of proteins on the microscopic level

Columbia, MO | Posted on December 17th, 2013

"Force microscopes are very different from the microscopes we used in biology class," said Gavin King, assistant professor of physics and astronomy in the College of Arts & Science at MU, and joint assistant professor of biochemistry. "Instead of using optics, force microscopes incorporate a tiny needle that gets dragged across the surface of the slide or specimen, similar to how a blind person reads Braille or comparable to the needle of an old record player. However, the one-dimensional, traditional method of studying membrane proteins through a force microscope—while good—only yields limited results," King said.

Normally, force microscopes measure the compression of the needle against the specimen by bouncing a single laser off the cantilever, or arm, that holds the microscopic needle in place. As the cantilever moves, it deflects light that is sent back to a highly advanced computer. There, the results are interpreted, giving researchers an idea of how the membrane proteins are interacting with the cell.

Usually, to determine membrane protein structure in detail, specimens must be crystallized, or frozen; therefore, the specimen cannot be studied as it would behave in the primarily liquid environment found in the body.

King and his fellow researcher, Krishna Sigdel, a postdoctoral fellow in the Department of Physics, solved the problem by building their own force microscope that is able to study membrane proteins in conditions similar to those found in the body. Using a traditional one-dimensional force microscope as a guide, the team added an additional laser that measures the second and third dimensions of tip movement, giving researchers "real-time" access to the measurement of peaks and valleys in the membrane protein and dynamic changes in those structures.

"By adding a new laser that is focused from below, we essentially gave the force microscope two additional dimensions," King said. "Using this new laser, we collect the back-scattered light from not only the cantilever holding the needle, but also the tip of the needle that gives additional measurements. This added flexibility allows us to collect information faster and allows our microscope to work in near-native conditions in fluid like those found in the cell, yielding more realistic results."

King suggested that an advantage of three-dimensional force microscopy is that it allows for better interpretation of how a protein's dynamic shape also dictates its function. King said that by studying how the shape of proteins change, researchers can determine how drugs bind and interact with cells. Using membrane protein information, pharmaceutical companies can determine which molecules to pursue.

King's work, "Three-dimensional atomic force microscopy: interaction force vector by direct observation of tip trajectory," was published in NanoLetters, the journal of the American Chemical Society and was funded in part by the National Science Foundation and the Burroughs Wellcome Fund.

The publication was co-written by King, Sigdel and Justin Grayer, who is currently a graduate student in MU's Electrical and Computer Engineering Department.

King's joint appointment in the Department of Biochemistry, which is located in the School of Medicine and the College of Agriculture, Food, and Natural Resources, emphasizes the highly collaborative culture in the area of One Health/One Medicine—one of the four key areas of collaborative strength that distinguish MU collectively known as the Mizzou Advantage. The other three areas are Food for the Future, Sustainable Energy, and Media of the Future.

####

For more information, please click here

Contacts:
Jeff Sossamon

573-882-3346

Copyright © University of Missouri-Columbia

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Dartmouth team creates new method to control quantum systems May 24th, 2016

Light can 'heal' defects in new solar cell materials: Defects in some new electronic materials can be removed by making ions move under illumination May 24th, 2016

Attosecond physics: A switch for light-wave electronics May 24th, 2016

Supercrystals with new architecture can enhance drug synthesis May 24th, 2016

Imaging

Light can 'heal' defects in new solar cell materials: Defects in some new electronic materials can be removed by making ions move under illumination May 24th, 2016

More light on cancer: Scientists created nanoparticles to highlight cancer cells May 21st, 2016

Videos/Movies

Programmable materials find strength in molecular repetition May 23rd, 2016

Graphene makes rubber more rubbery May 23rd, 2016

ORNL demonstrates large-scale technique to produce quantum dots May 21st, 2016

Discoveries

Dartmouth team creates new method to control quantum systems May 24th, 2016

Light can 'heal' defects in new solar cell materials: Defects in some new electronic materials can be removed by making ions move under illumination May 24th, 2016

Attosecond physics: A switch for light-wave electronics May 24th, 2016

Supercrystals with new architecture can enhance drug synthesis May 24th, 2016

Announcements

Dartmouth team creates new method to control quantum systems May 24th, 2016

Light can 'heal' defects in new solar cell materials: Defects in some new electronic materials can be removed by making ions move under illumination May 24th, 2016

Attosecond physics: A switch for light-wave electronics May 24th, 2016

Supercrystals with new architecture can enhance drug synthesis May 24th, 2016

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

Dartmouth team creates new method to control quantum systems May 24th, 2016

Light can 'heal' defects in new solar cell materials: Defects in some new electronic materials can be removed by making ions move under illumination May 24th, 2016

Attosecond physics: A switch for light-wave electronics May 24th, 2016

Supercrystals with new architecture can enhance drug synthesis May 24th, 2016

Tools

Light can 'heal' defects in new solar cell materials: Defects in some new electronic materials can be removed by making ions move under illumination May 24th, 2016

More light on cancer: Scientists created nanoparticles to highlight cancer cells May 21st, 2016

Nanotubes are beacons in cancer-imaging technique: Rice University researchers use spectral triangulation to pinpoint location of tumors May 21st, 2016

Carnegie Mellon develops bio-mimicry method for preparing and labeling stem cells: Method allows researchers to prepare mesenchymal stem cells and monitor them using MRI May 19th, 2016

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







Car Brands
Buy website traffic