Nanotechnology Now





Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Purdue Researchers Use Enzyme To Clip 'DNA Wires'

Abstract:
Enzymatic Clipping of DNA Wires Coated with Magnetic Nanoparticles

Purdue Researchers Use Enzyme To Clip 'DNA Wires'

West Lafayette, IN | February 28, 2005

Researchers at Purdue University have attached magnetic "nanoparticles" to DNA and then cut these "DNA wires" into pieces, offering the promise of creating low-cost, self-assembling devices for future computers.

Findings are detailed in a paper published online in February in the Journal of the American Chemical Society. The paper was written by Purdue graduate student Joseph M. Kinsella and Albena Ivanisevic, an assistant professor of biomedical engineering and chemistry at Purdue.

DNA, or deoxyribonucleic acid, has an overall negative charge, so it might be used in a process called self-assembly to create electronic devices. When placed in a solution with magnetic particles that have a positive charge, the particles are automatically attracted to the DNA strands, which act as tiny scaffolds for creating wires.

Click for large version.
Part A of this graphic shows a procedure for "templating" magnetic iron oxide nanoparticles onto DNA and stretching the DNA using a technique called molecular combing. Part B is an image taken with an atomic force microscope that shows a DNA strand coated with magnetic iron oxide nanoparticles. Researchers at Purdue University have shown how to attach the nanoparticles to DNA and use a "restriction enzyme" to cut the resulting "DNA wires" into pieces, offering the promise of creating low-cost, self-assembling devices for future computers.

Courtesy Purdue University, Weldon School of Biomedical Engineering.

Other researchers have "metalized" DNA by coating it with copper, gold and platinum, but no other researchers have coated DNA and then cut the strands into smaller pieces using a "restriction enzyme," a class of enzyme that causes DNA to fragment, Kinsella said.

Because magnetic components are essential for today's computer memory, the findings represent potential future applications for DNA-based structures in computers created with "molecular electronics," in which biological molecules might be harnesses to create devices for computers, sensors and other uses. Self-assembly might be used in the future to create electronic devices at lower cost than is possible with conventional manufacturing processes.

Purdue researchers had previously developed a technique for precisely placing strands of DNA on a silicon chip and then stretching out the strands so that their encoded information might be read more clearly. The current work by Ivanisevic's team builds on that previous research.

Kinsella created the magnetic particles, which are made from a ceramic iron oxide material about 4 nanometers in diameter. A nanometer is one billionth of a meter, or roughly 10 times the size of a hydrogen atom.

The Purdue researchers sliced the DNA wires with an enzyme called BamH1, one of numerous restriction enzymes that are used in standard genetic engineering techniques to snip DNA so that scientists can alter the genetic structures of organisms like bacteria.

DNA molecules contain "bases" called guanine, adenine, thymine and cytosine, represented as G, A, T and C. The bases combine in numerous sequences, and various restriction enzymes attach to and cut specific sequences, enabling scientists to isolate and snip DNA segments of differing lengths. The enzyme used in the Purdue research cuts segments of DNA containing a sequence of GGATCC.

"We incubate the particles and DNA in a solution, and the electric charge brings them together to form the wire," Ivanisevic said. "Then we basically make smaller wire segments with magnetic particles attached to this DNA sequence."

Because hundreds of different restriction enzymes snip segments containing specific sequences of genetic material, the method might be used in the future to cut DNA wires of varying lengths for building electronic devices.

Ivanisevic and former Purdue physics graduate student Dorjderem Nyamjav were the first to coat DNA with magnetic particles two years ago. Kinsella and Ivanisevic are the first to show that the BamH1 enzyme cuts DNA wires.

"We weren't sure the enzyme would be able to recognize the DNA sequence covered with particles," Kinsella said. "We thought the particles might hinder the process."

The researchers found, however, that the particles did not interfere with the process, possibly because the electrical charges are strong enough to hold the particles firmly in place, but weak enough to enable the enzyme to push them out of the way.

"The entire strand of DNA used in this research has been stretched onto silicon oxide surfaces at lengths up to 35 microns, or millionths of a meter, and 2 nanometers wide," Kinsella said. "When coated with particles and fragmented by the enzyme, we were able to distinguish that the once-single DNA wire was clipped into smaller wires."

In future work, the Purdue researchers plan to stretch DNA coated with magnetic particles between electrodes and test the coated genetic material for electrical properties.

The research is funded by the National Aeronautics and Space Administration through Purdue's NASA Institute for Nanoelectronics and Computing. The institute is a collaboration of six universities led by Purdue, whose director is Supriyo Datta, the Thomas Duncan Distinguished Professor of Electrical and Computer Engineering at Purdue. The work also is affiliated with the Birck Nanotechnology Center and the Bindley Bioscience Center in Purdue's Discovery Park, the university's hub for high-tech research.

Writer: Emil Venere, (765) 494-4709, venere@purdue.edu

Sources: Joseph Kinsella, (765) 496-6431, jkinsel@purdue.edu

Albena Ivanisevic, (765) 496-3676, albena@purdue.edu



Contact:
Purdue University
News Service
400 Centennial Mall Drive, Rm. 324
West Lafayette, IN 47907-2016
(765) 494-2096
fax: (765) 49400401
purduenews@purdue.edu

Copyright © Purdue University

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

Possible Futures

Simulations predict flat liquid May 21st, 2015

Nature inspires first artificial molecular pump: Simple design mimics pumping mechanism of life-sustaining proteins found in living cells May 19th, 2015

NNCO and Museum of Science Fiction to Collaborate on Nanotechnology and 3D Printing Panels at Awesome Con May 19th, 2015

Quantum 'gruyères' for spintronics of the future: Topological insulators become a little less 'elusive' May 12th, 2015

Molecular Machines

UCLA nanoscientists are first to model atomic structures of three bacterial nanomachines: Cryo electron microscope enables scientists to explore the frontiers of targeted antibiotics April 21st, 2015

Advances in molecular electronics: Lights on -- molecule on: Researchers from Dresden and Konstanz succeed in light-controlled molecule switching April 20th, 2015

Designer's toolkit for dynamic DNA nanomachines: Arm-waving nanorobot signals new flexibility in DNA origami March 27th, 2015

Tiny bio-robot is a germ suited-up with graphene quantum dots March 24th, 2015

Self Assembly

Scientists Use Nanoscale Building Blocks and DNA 'Glue' to Shape 3D Superlattices: New approach to designing ordered composite materials for possible energy applications April 23rd, 2015

Advances in molecular electronics: Lights on -- molecule on: Researchers from Dresden and Konstanz succeed in light-controlled molecule switching April 20th, 2015

Carnegie Mellon chemists create tiny gold nanoparticles that reflect nature's patterns April 9th, 2015

DWI scientists program the lifetime of self-assembled nanostructures April 9th, 2015

Nanoelectronics

Random nanowire configurations increase conductivity over heavily ordered configurations May 16th, 2015

Channeling valleytronics in graphene: Berkeley Lab researchers discover 1-D conducting channels in bilayer graphene May 6th, 2015

A better way to build DNA scaffolds: McGill researchers devise new technique to produce long, custom-designed DNA strands May 6th, 2015

Surface matters: Huge reduction of heat conduction observed in flat silicon channels April 23rd, 2015

Discoveries

Visualizing How Radiation Bombardment Boosts Superconductivity: Atomic-level flyovers show how impact sites of high-energy ions pin potentially disruptive vortices to keep high-current superconductivity flowing May 23rd, 2015

Conversion of Greenhouse Gases to Syngas in Presence of Nanocatalysts in Iran May 22nd, 2015

New Antibacterial Wound Dressing in Iran Can Display Replacement Time May 22nd, 2015

Nanotherapy effective in mice with multiple myeloma May 21st, 2015

Announcements

Visualizing How Radiation Bombardment Boosts Superconductivity: Atomic-level flyovers show how impact sites of high-energy ions pin potentially disruptive vortices to keep high-current superconductivity flowing May 23rd, 2015

Conversion of Greenhouse Gases to Syngas in Presence of Nanocatalysts in Iran May 22nd, 2015

New Antibacterial Wound Dressing in Iran Can Display Replacement Time May 22nd, 2015

Haydale Named Lead Sponsor for Cambridge Graphene Festival May 22nd, 2015

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project