Nanotechnology Now

Our NanoNews Digest Sponsors
Heifer International

Wikipedia Affiliate Button

Home > Press > Measuring the nanoworld

Researchers from around the world established a benchmark for the FRET technology by measuring distances within DNA molecules with sub-nanometer precision.

CREDIT
Source: Hugo Sanabria, Nandakumar Chedikulathu Vishnu/Universität Clemson
Researchers from around the world established a benchmark for the FRET technology by measuring distances within DNA molecules with sub-nanometer precision. CREDIT Source: Hugo Sanabria, Nandakumar Chedikulathu Vishnu/Universität Clemson

Abstract:
A worldwide study involving 20 laboratories has established and standardized a method to measure exact distances within individual biomolecules, down to the scale of one millionth of the width of a human hair. The new method represents a major improvement of a technology called single-molecule FRET (Förster Resonance Energy Transfer), in which the movement and interaction of fluorescently labelled molecules can be monitored in real time even in living cells. So far, the technology has mainly been used to report changes in relative distances - for instance, whether the molecules moved closer together or farther apart. Prof. Dr. Thorsten Hugel of the Institute of Physical Chemistry and the BIOSS Centre for Biological Signalling Studies is one of the lead scientists of the study, which was recently published in Nature Methods.

Measuring the nanoworld

Freiburg, Germany | Posted on September 4th, 2018

FRET works similarly to proximity sensors in cars: the closer the object is, the louder or more frequent the beeps become. Instead of relying on acoustics, FRET is based on proximity-dependent changes in the fluorescent light emitted from two dyes and is detected by sensitive microscopes. The technology has revolutionised the analysis of the movement and interactions of biomolecules in living cells.

Hugel and colleagues envisioned that once a FRET standard had been established, unknown distances could be determined with high confidence. By working together, the 20 laboratories involved in the study refined the method in such a way that scientists using different microscopes and analysis software obtained the same distances, even in the sub-nanometer range.

"The absolute distance information that can be acquired with this method now enables us to accurately assign conformations in dynamic biomolecules, or even to determine their structures", says Thorsten Hugel, who headed the study together with Dr. Tim Craggs (University of Sheffield/Great-Britain), Prof. Dr. Claus Seidel (University of Düsseldorf) and Prof. Dr. Jens Michaelis (University of Ulm). Such dynamic structural information will yield a better understanding of the molecular machines and processes that are the basis of life.

####

For more information, please click here

Contacts:
Prof. Dr. Thorsten Hugel

Copyright © University of Freiburg

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

Original publication:

Related News Press

News and information

Perfect diamagnetism observation of high-temperature superconductivity in compressed H2S June 14th, 2019

Mysterious Majorana quasiparticle is now closer to being controlled for quantum computing: Princeton researchers detect a robust Majorana quasiparticle and show how it can be turned on and off June 14th, 2019

University of Konstanz researchers create uniform-shape polymer nanocrystals: Researchers from the University of Konstanz's CRC 1214 'Anisotropic Particles as Building Blocks: Tailoring Shape, Interactions and Structures' generate uniform-shape nanocrystals using direct polymeriz June 14th, 2019

Small currents for big gains in spintronics: A new low-power magnetic switching component could aid spintronic devices June 14th, 2019

Imaging

New Video Highlights Specific Topics Sought in Call for Papers for the 2019 IEEE International Electron Devices Meeting (IEDM) June 13th, 2019

2D crystals conforming to 3D curves create strain for engineering quantum devices June 7th, 2019

New Argonne coating could have big implications for lithium batteries May 14th, 2019

Better microring sensors for optical applications May 10th, 2019

Possible Futures

Perfect diamagnetism observation of high-temperature superconductivity in compressed H2S June 14th, 2019

Mysterious Majorana quasiparticle is now closer to being controlled for quantum computing: Princeton researchers detect a robust Majorana quasiparticle and show how it can be turned on and off June 14th, 2019

University of Konstanz researchers create uniform-shape polymer nanocrystals: Researchers from the University of Konstanz's CRC 1214 'Anisotropic Particles as Building Blocks: Tailoring Shape, Interactions and Structures' generate uniform-shape nanocrystals using direct polymeriz June 14th, 2019

Small currents for big gains in spintronics: A new low-power magnetic switching component could aid spintronic devices June 14th, 2019

Molecular Machines

DNA origami to scale-up molecular motors June 13th, 2019

Big energy savings for tiny machines May 24th, 2019

Kanazawa University research: A closed cage-like molecule that can be opened January 22nd, 2019

Insights into magnetic bacteria may guide research into medical nanorobots December 12th, 2018

Molecular Nanotechnology

DNA origami to scale-up molecular motors June 13th, 2019

Big energy savings for tiny machines May 24th, 2019

2D gold quantum dots are atomically tunable with nanotubes April 11th, 2019

The feature size and functional range of molecular electronic devices: Monitoring the transition from tunneling leakage current to molecular tunneling December 16th, 2018

Sensors

New Video Highlights Specific Topics Sought in Call for Papers for the 2019 IEEE International Electron Devices Meeting (IEDM) June 13th, 2019

Kanazawa University research: Opposite piezoresistant effects of rhenium disulfide in two principle directions June 13th, 2019

Shaking hands with human or robot? Nanotubes make them alike as never before June 6th, 2019

Good vibrations: Using piezoelectricity to ensure hydrogen sensor sensitivity May 24th, 2019

Discoveries

Perfect diamagnetism observation of high-temperature superconductivity in compressed H2S June 14th, 2019

Mysterious Majorana quasiparticle is now closer to being controlled for quantum computing: Princeton researchers detect a robust Majorana quasiparticle and show how it can be turned on and off June 14th, 2019

University of Konstanz researchers create uniform-shape polymer nanocrystals: Researchers from the University of Konstanz's CRC 1214 'Anisotropic Particles as Building Blocks: Tailoring Shape, Interactions and Structures' generate uniform-shape nanocrystals using direct polymeriz June 14th, 2019

Small currents for big gains in spintronics: A new low-power magnetic switching component could aid spintronic devices June 14th, 2019

Announcements

Perfect diamagnetism observation of high-temperature superconductivity in compressed H2S June 14th, 2019

Mysterious Majorana quasiparticle is now closer to being controlled for quantum computing: Princeton researchers detect a robust Majorana quasiparticle and show how it can be turned on and off June 14th, 2019

University of Konstanz researchers create uniform-shape polymer nanocrystals: Researchers from the University of Konstanz's CRC 1214 'Anisotropic Particles as Building Blocks: Tailoring Shape, Interactions and Structures' generate uniform-shape nanocrystals using direct polymeriz June 14th, 2019

Small currents for big gains in spintronics: A new low-power magnetic switching component could aid spintronic devices June 14th, 2019

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

Perfect diamagnetism observation of high-temperature superconductivity in compressed H2S June 14th, 2019

University of Konstanz researchers create uniform-shape polymer nanocrystals: Researchers from the University of Konstanz's CRC 1214 'Anisotropic Particles as Building Blocks: Tailoring Shape, Interactions and Structures' generate uniform-shape nanocrystals using direct polymeriz June 14th, 2019

Small currents for big gains in spintronics: A new low-power magnetic switching component could aid spintronic devices June 14th, 2019

Kanazawa University research: Opposite piezoresistant effects of rhenium disulfide in two principle directions June 13th, 2019

Research partnerships

2D crystals conforming to 3D curves create strain for engineering quantum devices June 7th, 2019

Shaking hands with human or robot? Nanotubes make them alike as never before June 6th, 2019

Beyond 1 and 0: Engineers boost potential for creating successor to shrinking transistors May 30th, 2019

Laser technique could unlock use of tough material for next-generation electronics: Researchers make graphene tunable, opening up its band gap to a record 2.1 electronvolts May 30th, 2019

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project