Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > JPK reports on research of the Mestroni Lab at the University of Colorado Denver which use the JPK NanoWizard® AFM to help in the characterization of cardiomyopathies

Experimental summary of mechanical measurements in the research of Ilaria Pecorari.
Experimental summary of mechanical measurements in the research of Ilaria Pecorari.

Abstract:
JPK Instruments, a world-leading manufacturer of nanoanalytic instrumentation for research in life sciences and soft matter, reports on the research of Professor Luisa Mestroni's research laboratory which specializes in the study of genetics of cardiac muscle diseases. They use the JPK NanoWizard® AFM to help the characterization of cardiomyopathies.

JPK reports on research of the Mestroni Lab at the University of Colorado Denver which use the JPK NanoWizard® AFM to help in the characterization of cardiomyopathies

Berlin, Germany | Posted on April 24th, 2018


Professor Luisa Mestroni heads a laboratory studying the genetics of cardiac muscle diseases. Based at the University of Colorado Denver and working in conjunction with labs in Italy, the lab's interest is in the genes causing dilated cardiomyopathy, arrhythmogenic right ventricular cardiomyopathy/dysplasia (ARVD/C), left ventricular noncompaction and hypertrophic cardiomyopathy.

One of the group's PhD students is Ilaria Pecorari. She is studying the effect of the scaffold on the cardiac cells cytoskeleton. This is mainly focused on the study of mechanical behaviour and mechanotransduction phenomenon in cells carrying a genetic mutation that is responsible for the onset of a pathological condition. She describes the study: “I am currently investigating the response of certain type of cells to different mechanical stimuli, i.e. exposure of cells to substrates with tunable stiffness, but I am also probing the mechanical response of “healthy” and mutant cells via atomic force microscopy (AFM). For the latter, I am exploiting the setup provided by JPK Instruments, in which the atomic force microscope (NanoWizard®4a BioSciences) is coupled with a fluorescence microscope. I infect cells with viral constructs, so they will express both green fluorescent protein (GFP) and the mutant protein known as the cause of disease. The NanoWizard® enables me to simultaneously identify the cells expressing the mutant protein and probe them mechanically. Through the force-deformation curves and their post-processing analysis, I can detect if the genetic mutation affects the mechanical behaviour of a single cell. In the near future, I'd like to assess the mechanical properties of wild type (i.e. “healthy”) and mutant cells on substrates with different rigidities (thus either stiff or soft).”

Describing her experiences with AFM, Ms Pecorari continued; “Having been a user of AFM for eight years, I first used an AFM from JPK in 2016 while working under the supervision of Dr José Luis Toca-Herrera at the BOKU University in Vienna (Austria). Compared to other equipment I have used, the NanoWizard® is extremely user friendly. It is very intuitive, once the working principle of atomic force microscopy is clear. The variety of modes available, included the quantitative imaging (QI™) mode, allows the user to acquire a large variety of data on a sample. While working with living cells, it is crucial to control the temperature setting and the JPK PetriDishHeater™ is reliable and easy to use. The software for the post-processing of data is also very easy to use and quite automatic. So overall, I think that working with JPK’s AFM has guaranteed a high degree of reliability of my observations.”

This work has been published in a review article in Seminars in Cell & Development Biology published by Elsevier in 2017. The group has also published an interesting paper where the AFM is used to monitor the beating of cardiomyocytes grown on a 3D carbon nanotube scaffold. The lead author is Dr Brisa Peńa and is published in the ACS' Applied Materials & Interfaces. It may be viewed here.

For more details about JPK’s AFM systems and their applications for the materials, life & nano sciences, please contact JPK on +49 30726243 500. Alternatively, please visit the web site: http://www.jpk.com/ or see more on Facebook: www.jpk.com/facebook and on You Tube: http://www.youtube.com/jpkinstruments.

References
Lui et al, Cyclophilin A stabilizes the HIV-1 capsid through a novel non-canonical binding site, NATURE COMMUNICATIONS | 7:10714 | DOI: 10.1038/ncomms10714

Ramalho et al, Analysis of the mechanical properties of wild type and hyperstable mutants of the HIV‑1 capsid, Retrovirology (2016) 13:17 DOI 10.1186/s12977-016-0250-4

Rankovic et al, Reverse Transcription Mechanically Initiates HIV-1 Capsid Disassembly, J Virol 91:e00289-17. https://doi.org/10.1128/JVI.00289-17 .

####

About JPK Instruments
JPK Instruments AG is a world-leading manufacturer of nanoanalytic instruments - particularly atomic force microscope (AFM) systems and optical tweezers - for a broad range of applications reaching from soft matter physics to nano-optics, from surface chemistry to cell and molecular biology. From its earliest days applying atomic force microscope (AFM) technology, JPK has recognized the opportunities provided by nanotechnology for transforming life sciences and soft matter research. This focus has driven JPK’s success in uniting the worlds of nanotechnology tools and life science applications by offering cutting-edge technology and unique applications expertise. Headquartered in Berlin and with direct operations in Dresden, Cambridge (UK), Singapore, Tokyo, Shanghai (China), Paris (France) and Carpinteria (USA), JPK maintains a global network of distributors and support centers and provides on the spot applications and service support to an ever-growing community of researchers.

For more information, please click here

Contacts:
JPK Instruments AG
Colditzstrasse 34-36
Haus 13, Eingang B
Berlin 12099
Germany
T +49 30726243 500
F +49 30726243 999
http://www.jpk.com/


Talking Science Limited
39 de Bohun Court
Saffron Walden
Essex CB10 2BA UK
T +44(0)1799 521881
M +44(0)7843 012997

www.talking-science.com.

Copyright © JPK Instruments

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Switching with molecules: Molecular switch will facilitate the development of pioneering electro-optical devices May 25th, 2018

Tunable diamond string may hold key to quantum memory: A process similar to guitar tuning improves storage time of quantum memory May 24th, 2018

Remote control of transport through nanopores: New study outlines key factors affecting the transfer of molecules through biological channels May 24th, 2018

2018 Kavli Prizes in Astrophysics, Nanoscience, and Neuroscience to be Announced Live on May 31: Live announcement at the Norwegian Academy of Science and Letters to be streamed live at World Science Festival Event May 24th, 2018

Imaging

Columbia Researchers Squeeze Light into Nanoscale Devices and Circuits: Team is first to directly image propagation and dynamics of graphene plasmons at very low temperatures; findings could impact optical communications and signal processing May 23rd, 2018

NIST Puts the Optical Microscope Under the Microscope to Achieve Atomic Accuracy May 22nd, 2018

Elastic microspheres expand understanding of embryonic development and cancer cells May 15th, 2018

Nanoscale measurements 100x more precise, thanks to improved two-photon technique May 8th, 2018

Possible Futures

Switching with molecules: Molecular switch will facilitate the development of pioneering electro-optical devices May 25th, 2018

Tunable diamond string may hold key to quantum memory: A process similar to guitar tuning improves storage time of quantum memory May 24th, 2018

Remote control of transport through nanopores: New study outlines key factors affecting the transfer of molecules through biological channels May 24th, 2018

'Spooky action at a distance': Researchers develop module for quantum repeater May 23rd, 2018

Nanomedicine

Remote control of transport through nanopores: New study outlines key factors affecting the transfer of molecules through biological channels May 24th, 2018

New blood test rapidly detects signs of pancreatic cancer May 17th, 2018

Elastic microspheres expand understanding of embryonic development and cancer cells May 15th, 2018

Nanomedicine -- Targeting cancer cells with sugars May 14th, 2018

Discoveries

Switching with molecules: Molecular switch will facilitate the development of pioneering electro-optical devices May 25th, 2018

Tunable diamond string may hold key to quantum memory: A process similar to guitar tuning improves storage time of quantum memory May 24th, 2018

Remote control of transport through nanopores: New study outlines key factors affecting the transfer of molecules through biological channels May 24th, 2018

'Spooky action at a distance': Researchers develop module for quantum repeater May 23rd, 2018

Announcements

Switching with molecules: Molecular switch will facilitate the development of pioneering electro-optical devices May 25th, 2018

Tunable diamond string may hold key to quantum memory: A process similar to guitar tuning improves storage time of quantum memory May 24th, 2018

Remote control of transport through nanopores: New study outlines key factors affecting the transfer of molecules through biological channels May 24th, 2018

2018 Kavli Prizes in Astrophysics, Nanoscience, and Neuroscience to be Announced Live on May 31: Live announcement at the Norwegian Academy of Science and Letters to be streamed live at World Science Festival Event May 24th, 2018

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

Switching with molecules: Molecular switch will facilitate the development of pioneering electro-optical devices May 25th, 2018

Tunable diamond string may hold key to quantum memory: A process similar to guitar tuning improves storage time of quantum memory May 24th, 2018

Remote control of transport through nanopores: New study outlines key factors affecting the transfer of molecules through biological channels May 24th, 2018

'Spooky action at a distance': Researchers develop module for quantum repeater May 23rd, 2018

Tools

Columbia Researchers Squeeze Light into Nanoscale Devices and Circuits: Team is first to directly image propagation and dynamics of graphene plasmons at very low temperatures; findings could impact optical communications and signal processing May 23rd, 2018

NIST Puts the Optical Microscope Under the Microscope to Achieve Atomic Accuracy May 22nd, 2018

Self-assembling 3D battery would charge in seconds May 22nd, 2018

A micro-thermometer to record tiny temperature changes May 15th, 2018

Nanobiotechnology

Remote control of transport through nanopores: New study outlines key factors affecting the transfer of molecules through biological channels May 24th, 2018

New blood test rapidly detects signs of pancreatic cancer May 17th, 2018

Nanomedicine -- Targeting cancer cells with sugars May 14th, 2018

NanoBio Announces Corporate Name Change to BlueWillow Biologics and Closes $10M Series A Financing: Move Reflects Focus on Advancing Several Intranasal Vaccines to Human Studies May 9th, 2018

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project