Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > The Catholic University of Rome uses the JPK NanoWizard® AFM & CellHesion® systems to understand how cells sense and respond to mechanical stimuli

Examples of cell tissue modulus vs frequency maps obtained using the JPK NanoWizard® and CellHesion® systems
Examples of cell tissue modulus vs frequency maps obtained using the JPK NanoWizard® and CellHesion® systems

Abstract:
JPK Instruments, a world-leading manufacturer of nanoanalytic instrumentation for research in life sciences and soft matter, reports on the work of Professor Marco De Spirito's research group at the Catholic University of Rome. The group uses a NanoWizard® AFM and CellHesion® module to study how cells sense and respond to mechanical stimuli.

The Catholic University of Rome uses the JPK NanoWizard® AFM & CellHesion® systems to understand how cells sense and respond to mechanical stimuli

Berlin, Germany | Posted on April 5th, 2017

Dr Gabriele Ciasca and Professor Massimiliano Papi are members of the research team of Professor Marco De Spirito in the Institute of Physics at the Catholic University of Rome, Italy. One of the main goals of their group is the investigation of how cells sense and respond to physical and mechanical stimuli. Professor De Spirito says that a deeper knowledge of cell biomechanics can boost the understanding of how mechanical properties affect and are affected by the development of many pathological states including cancer.

An example of this research has been reported in a recent clinical paper published in the high impact factor journal Nanoscale. This paper, “Nano-mechanical signature of brain tumours,” was carried out in collaboration with Dr Tanya Enny Sassun during her PhD in the group of Professor Delfini, head of the Department of Neurology and Psychiatry, Neurosurgery (Sapienza University of Rome). The research group studied the biomechanical fingerprint of the two most frequent malignant and benign brain tumours: the highly aggressive Glioblastoma and the slowly-growing Meningioma. They investigated the complex biophysical interplay between neoplastic cells and the tumour microenvironment using the NanoWizard® AFM from JPK. This showed that AFM is able to easily distinguish between cancerous and healthy peritumoural tissues.

Eleonora Minelli - who works as a PhD student in the group of Professor De Spirito - takes up the story of how this work has been extended. “The acquisition of elasticity maps of surgically removed tissues is plagued by the problem of roughness that is often larger than the available range of the piezoelectric actuator. This meant we have had to develop a novel procedure that allowed us to acquire elasticity maps of an unparalleled size (up to 100 µm x 100 µm). We achieved this result thanks to the use of the JPK CellHesion® module that can be easily integrated to our NanoWizard®. This has a z-piezoelectric actuator with a range of 100 µm. These results open up many applications in nanomedicine and have the potential to boost the use of AFM in clinical practice. AFM, together with confocal microscopy and electron microscopy, are key tools in this research area because it allows us to probe mechanical and topographical properties of molecules, cells and tissues in nearly all environments.”

Dr Ciasca, Professor Papi and their colleagues have a lot of experience using different makes of AFM. “The members of our group have been working with many general-purpose AFM set-ups. Now, we are deeply convinced that the JPK NanoWizard® offers one of the best suited experimental set-ups for the investigation of biological systems. There are a number of reasons for this. The instrument has an easy, accurate and effective cantilever calibration procedure. We believe this is a key advantage of this platform as it ensures reproducibility and reliability of results. This is particularly important when dealing with the nanoscale mechanical properties of cells and tissues that are intrinsically subjected to a large biological variability. The geometry of the scanning head is a unique characteristic of the JPK NanoWizard®. It opens the possibility to investigate cells and tissues directly within conventional petri dishes in a liquid environment. This key characteristic allowed us to investigate the mechanical and structural properties of living cells in their own environment without the need of fixation procedures that deeply alter mechanical and morphological properties. Most importantly, the NanoWizard® in our laboratory offers effective integration with a conventional inverted fluorescence microscopy which allows us to combine fluorescence and optical images with elasticity maps.”

The Group publishes extensively. Some of their most recent key publications include:

Nano-mechanical signature of brain tumours (Nanoscale 8 (47), 19629-19643) by G Ciasca et al.

Mapping viscoelastic properties of healthy and pathological red blood cells at the nanoscale level (Nanoscale, 2015,7, 17030-17037 DOI: 10.1039/C5NR03145A) by G Ciasca et al.

Bacteria Meet Graphene: Modulation of Graphene Oxide Nanosheet Interaction with Human Pathogens for Effective Antimicrobial Therapy (ACS Biomaterials Science & Engineering, 2017) by V Palmieri et al.

Mechanical and structural comparison between primary tumor and lymph node metastasis cells in colorectal cancer (Soft Matter, 2015,11, 5719-5726 DOI: 10.1039/C5SM01089F) by Dr V Palmieri et al.

For more details about JPK's AFM systems and their applications for the materials, life & nano sciences, please contact JPK on +49 30726243 500. Alternatively, please visit the web site: www.jpk.com/ or see more on Facebook: www.jpk.com/facebook and on You Tube: www.youtube.com/jpkinstruments.

####

About JPK Instruments
JPK Instruments AG is a world-leading manufacturer of nanoanalytic instruments - particularly atomic force microscope (AFM) systems and optical tweezers - for a broad range of applications reaching from soft matter physics to nano-optics, from surface chemistry to cell and molecular biology. From its earliest days applying atomic force microscope (AFM) technology, JPK has recognized the opportunities provided by nanotechnology for transforming life sciences and soft matter research. This focus has driven JPK's success in uniting the worlds of nanotechnology tools and life science applications by offering cutting-edge technology and unique applications expertise. Headquartered in Berlin and with direct operations in Dresden, Cambridge (UK), Singapore, Tokyo, Shanghai (China), Paris (France) and Carpinteria (USA), JPK maintains a global network of distributors and support centers and provides on the spot applications and service support to an ever-growing community of researchers.

For more information, please click here

Contacts:
JPK Instruments AG
Colditzstrasse 34-36
Haus 13, Eingang B
Berlin 12099
Germany
T +49 30726243 500
F +49 30726243 999
http://www.jpk.com/


Talking Science Limited
39 de Bohun Court
Saffron Walden
Essex CB10 2BA UK
T +44(0)1799 521881
M +44(0)7843 012997
www.talking-science.com.

Copyright © JPK Instruments

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Atomic imperfections move quantum communication network closer to reality June 25th, 2017

Research accelerates quest for quicker, longer-lasting electronics: UC Riverside-led research makes topological insulators magnetic well above room temperatures June 25th, 2017

U.S. Air Force Research Lab Taps IBM to Build Brain-Inspired AI Supercomputing System: Equal to 64 million neurons, new neurosynaptic supercomputing system will power complex AI tasks at unprecedented speed and energy efficiency June 23rd, 2017

Rice U. chemists create 3-D printed graphene foam June 22nd, 2017

Imaging

Researchers developed nanoparticle based contrast agent for dual modal imaging of cancer June 21st, 2017

Cambridge Nanotherm partners with Inabata for global sales and distribution June 20th, 2017

GLOBALFOUNDRIES Launches 7nm ASIC Platform for Data Center, Machine Learning, and 5G Networks FX-7TM offering leverages the company’s 7nm: FinFET process to deliver best in class IP and Solutions June 13th, 2017

The Zeiss Global Centre in the School of Engineering at the University of Portsmouth uses Deben µXCT stages to characterise the structural competence of biological structures June 13th, 2017

Academic/Education

Oxford Instruments congratulates Lancaster University for inaugurating the IsoLab, built for studying quantum systems June 20th, 2017

The 2017 Winners for Generation Nano June 8th, 2017

MIT Energy Initiative awards 10 seed fund grants for early-stage energy research May 4th, 2017

Bar-Ilan University to set up quantum research center May 1st, 2017

Nanomedicine

Researchers developed nanoparticle based contrast agent for dual modal imaging of cancer June 21st, 2017

Learning with light: New system allows optical “deep learning”: Neural networks could be implemented more quickly using new photonic technology June 12th, 2017

Mussels add muscle to biocompatible fibers: Rice University chemists develop hydrogel strings using compound found in sea creatures June 9th, 2017

Making vessels leaky on demand could aid drug delivery:Rice University scientists use magnets and nanoparticles to open, close gaps in blood vessels June 8th, 2017

Discoveries

Atomic imperfections move quantum communication network closer to reality June 25th, 2017

Research accelerates quest for quicker, longer-lasting electronics: UC Riverside-led research makes topological insulators magnetic well above room temperatures June 25th, 2017

Rice U. chemists create 3-D printed graphene foam June 22nd, 2017

Enhanced photocatalytic activity by Cu2O nanoparticles integrated H2Ti3O7 nanotubes June 21st, 2017

Announcements

Atomic imperfections move quantum communication network closer to reality June 25th, 2017

Research accelerates quest for quicker, longer-lasting electronics: UC Riverside-led research makes topological insulators magnetic well above room temperatures June 25th, 2017

U.S. Air Force Research Lab Taps IBM to Build Brain-Inspired AI Supercomputing System: Equal to 64 million neurons, new neurosynaptic supercomputing system will power complex AI tasks at unprecedented speed and energy efficiency June 23rd, 2017

Rice U. chemists create 3-D printed graphene foam June 22nd, 2017

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

Atomic imperfections move quantum communication network closer to reality June 25th, 2017

Research accelerates quest for quicker, longer-lasting electronics: UC Riverside-led research makes topological insulators magnetic well above room temperatures June 25th, 2017

Rice U. chemists create 3-D printed graphene foam June 22nd, 2017

Tiny bubbles provide tremendous propulsion in new microparticles research-Ben-Gurion U. June 21st, 2017

Tools

Researchers developed nanoparticle based contrast agent for dual modal imaging of cancer June 21st, 2017

Oxford Instruments congratulates Lancaster University for inaugurating the IsoLab, built for studying quantum systems June 20th, 2017

Changing the color of laser light on the femtosecond time scale: How BiCoO3 achieves second harmonic generation June 14th, 2017

Leti Announces Two New Tools for Improving Transportation Comfort, Safety and Efficiency: Wearable Device Measures Stress Responses for Travelers, Pilots and Truck Drivers, While Smartphone App Provides Transit Agencies Broad Data on Transport Modes June 13th, 2017

Nanobiotechnology

Researchers developed nanoparticle based contrast agent for dual modal imaging of cancer June 21st, 2017

Mussels add muscle to biocompatible fibers: Rice University chemists develop hydrogel strings using compound found in sea creatures June 9th, 2017

Making vessels leaky on demand could aid drug delivery:Rice University scientists use magnets and nanoparticles to open, close gaps in blood vessels June 8th, 2017

Nanobiotix's promising data from Phase I/II head and neck cancer trial presented at ASCO June 5th, 2017

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project