Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > Carnegie Mellon Chemists Create Nanofibers Using Unprecedented New Method

Abstract:
Researchers from Carnegie Mellon University have developed a novel method for creating self-assembled protein/polymer nanostructures that are reminiscent of fibers found in living cells. The work offers a promising new way to fabricate materials for drug delivery and tissue engineering applications. The findings were published in the July 28 issue of Angewandte Chemie International Edition.

Carnegie Mellon Chemists Create Nanofibers Using Unprecedented New Method

Pittsburgh, PA | Posted on July 31st, 2014

"We have demonstrated that, by adding flexible linkers to protein molecules, we can form completely new types of aggregates. These aggregates can act as a structural material to which you can attach different payloads, such as drugs. In nature, this protein isn't close to being a structural material," said Tomasz Kowalewski, professor of chemistry in Carnegie Mellon's Mellon College of Science.

The building blocks of the fibers are a few modified green fluorescent protein (GFP) molecules linked together using a process called click chemistry. An ordinary GFP molecule does not normally bind with other GFP molecules to form fibers. But when Carnegie Mellon graduate student Saadyah Averick, working under the guidance of Krzysztof Matyjaszewski, the J.C. Warner Professor of Natural Sciences and University Professor of Chemistry in CMU's Mellon College of Science, modified the GFP molecules and attached PEO-dialkyne linkers to them, they noticed something strange — the GFP molecules appeared to self-assemble into long fibers. Importantly, the fibers disassembled after being exposed to sound waves, and then reassembled within a few days. Systems that exhibit this type of reversible fibrous self-assembly have been long sought by scientists for use in applications such as tissue engineering, drug delivery, nanoreactors and imaging.

"This was purely curiosity-driven and serendipity-driven work," Kowalewski said. "But where controlled polymerization and organic chemistry meet biology, interesting things can happen."

The research team observed the fibers using confocal light microscopy, confirmed their assembly using dynamic light scattering and studied their morphology using atomic force microscopy (AFM). They also observed that the fibers were fluorescent, indicating that the GFP molecules retained their 3-D structure while linked together.

To determine what processes were driving the self-assembly, Matyjaszewski and Kowalewski turned to Anna Balazs, Distinguished Professor of Chemical Engineering and the Robert v. d. Luft Professor at the University of Pittsburgh. A leading expert in modeling the dynamics and mechanical properties of mesoscale systems, Balazs ran a computer simulation of the GFP molecules' self-assembly process using a technique called dissipative particle dynamics, a type of coarse-grained molecular dynamics method. The simulation confirmed the modified GFP's tendency to form fibers and revealed that the self-assembly process was driven by the interaction of hydrophobic patches on the surfaces of individual GFP molecules. In addition, Balazs's simulated fibers closely corresponded with what Kowalewski observed using AFM imaging.

"Our protein-polymer system gives us an atomically precise, very well-defined nanoscale building object onto which we can attach different handles in very precisely defined positions. It can be used in a way that wasn't ever intended by biology," Kowalewski said.

In addition to Averick, Balazs, Kowalewski and Matyjaszewski, co-authors of the study include Carnegie Mellon's Orsolya Karacsony and Jacob Mohin, University of Pittsburgh's Xin Yong and Nicholas M. Moellers, Oregon State University's Bradley F. Woodman and Ryan A. Mehl, and Zhejiang University's Weipu Zhu. The research was supported by the U.S. Department of Energy, National Science Foundation, Carnegie Mellon's CRP Consortium and Oregon State University.

####

For more information, please click here

Contacts:
Jocelyn Duffy

412-268-9982

Copyright © Carnegie Mellon University

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Photonic chip guides single photons, even when there are bends in the road February 16th, 2018

Arrowhead Receives Regulatory Clearance to Begin Phase 1/2 Study of ARO-HBV for Treatment of Hepatitis B February 15th, 2018

Arrowhead Pharmaceuticals Receives Orphan Drug Designation for ARO-AAT February 15th, 2018

European & Korean Project To Demo World’s First 5G Platform During Winter Games February 15th, 2018

Imaging

Graphene on toast, anyone? Rice University scientists create patterned graphene onto food, paper, cloth, cardboard February 13th, 2018

New method enables high-resolution measurements of magnetism February 7th, 2018

Leti Presents Optical-Equipment Curving Technology that Improves Performance, Cuts Costs: ‘Disruptive Approach’ for Imaging Applications Presented in Paper At Photonics West and Demonstrated in Leti’s Booth February 2nd, 2018

New technology aiming to improve trueness in the piezoelectric microscopy characterization of ceramic materials January 26th, 2018

Synthetic Biology

Synthetic protein packages its own genetic material and evolves computationally designed protein assemblies are advancing research in synthetic life and in targeted drug delivery December 15th, 2017

Report highlights opportunities and risks associated with synthetic biology and bioengineering November 22nd, 2017

Living computers: RNA circuits transform cells into nanodevices July 27th, 2017

In-cell molecular sieve from protein crystal February 14th, 2017

Govt.-Legislation/Regulation/Funding/Policy

Arrowhead Receives Regulatory Clearance to Begin Phase 1/2 Study of ARO-HBV for Treatment of Hepatitis B February 15th, 2018

Arrowhead Pharmaceuticals Receives Orphan Drug Designation for ARO-AAT February 15th, 2018

Rutgers-Led Innovation Could Spur Faster, Cheaper, Nano-Based Manufacturing: Scalable and cost-effective manufacturing of thin film devices February 14th, 2018

Understanding brain functions using upconversion nanoparticles: Researchers can now send light deep into the brain to study neural activities February 14th, 2018

Self Assembly

Liquid crystal molecules form nano rings: Quantized self-assembly enables design of materials with novel properties February 7th, 2018

Particle size matters for porous building blocks: Rice University scientists find porous nanoparticles get tougher under pressure, but not when assembled December 19th, 2017

Physicists gain new insights into nanosystems with spherical confinement: Enormous potential for the targeted delivery of pharmaceutical agents and the creation of tailored nanoparticles July 27th, 2017

Oddball enzyme provides easy path to synthetic biomaterials May 17th, 2017

Discoveries

Photonic chip guides single photons, even when there are bends in the road February 16th, 2018

'Living bandages': NUST MISIS scientists develop biocompatible anti-burn nanofibers February 15th, 2018

Rutgers-Led Innovation Could Spur Faster, Cheaper, Nano-Based Manufacturing: Scalable and cost-effective manufacturing of thin film devices February 14th, 2018

Understanding brain functions using upconversion nanoparticles: Researchers can now send light deep into the brain to study neural activities February 14th, 2018

Materials/Metamaterials

Rutgers-Led Innovation Could Spur Faster, Cheaper, Nano-Based Manufacturing: Scalable and cost-effective manufacturing of thin film devices February 14th, 2018

Graphene on toast, anyone? Rice University scientists create patterned graphene onto food, paper, cloth, cardboard February 13th, 2018

Atomic Flaws Create Surprising, High-Efficiency UV LED Materials: Subtle surface defects increase UV light emission in greener, more cost-effective LED and catalyst materials February 8th, 2018

A new radiation detector made from graphene: A new bolometer exploits the thermoelectric properties of graphene February 6th, 2018

Announcements

Photonic chip guides single photons, even when there are bends in the road February 16th, 2018

Arrowhead Receives Regulatory Clearance to Begin Phase 1/2 Study of ARO-HBV for Treatment of Hepatitis B February 15th, 2018

Arrowhead Pharmaceuticals Receives Orphan Drug Designation for ARO-AAT February 15th, 2018

European & Korean Project To Demo World’s First 5G Platform During Winter Games February 15th, 2018

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

Photonic chip guides single photons, even when there are bends in the road February 16th, 2018

'Living bandages': NUST MISIS scientists develop biocompatible anti-burn nanofibers February 15th, 2018

Rutgers-Led Innovation Could Spur Faster, Cheaper, Nano-Based Manufacturing: Scalable and cost-effective manufacturing of thin film devices February 14th, 2018

Understanding brain functions using upconversion nanoparticles: Researchers can now send light deep into the brain to study neural activities February 14th, 2018

Tools

New method enables high-resolution measurements of magnetism February 7th, 2018

Nanometrics Selected for Fab-Wide Process Control Metrology by Domestic China 3D-NAND Manufacturer: Latest Fab Win Includes Comprehensive Suite for Substrate, Thin Film and Critical Dimension Metrology February 7th, 2018

A new radiation detector made from graphene: A new bolometer exploits the thermoelectric properties of graphene February 6th, 2018

Measuring the temperature of two-dimensional materials at the atomic level February 3rd, 2018

Research partnerships

Rutgers-Led Innovation Could Spur Faster, Cheaper, Nano-Based Manufacturing: Scalable and cost-effective manufacturing of thin film devices February 14th, 2018

Understanding brain functions using upconversion nanoparticles: Researchers can now send light deep into the brain to study neural activities February 14th, 2018

Vanadium dioxyde: A revolutionary material for tomorrow's electronics: Phase-chance switch can now be performed at higher temperatures February 5th, 2018

Nanowire LED Innovator Aledia Announces €30 ($36M) Million Series-C Financing: Intel Capital Joins Existing Investors to Commercialize Certain Nanowire-LED Technologies for Mobile Displays January 29th, 2018

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project