Nanotechnology Now







Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Carnegie Mellon Chemists Create Nanofibers Using Unprecedented New Method

Abstract:
Researchers from Carnegie Mellon University have developed a novel method for creating self-assembled protein/polymer nanostructures that are reminiscent of fibers found in living cells. The work offers a promising new way to fabricate materials for drug delivery and tissue engineering applications. The findings were published in the July 28 issue of Angewandte Chemie International Edition.

Carnegie Mellon Chemists Create Nanofibers Using Unprecedented New Method

Pittsburgh, PA | Posted on July 31st, 2014

"We have demonstrated that, by adding flexible linkers to protein molecules, we can form completely new types of aggregates. These aggregates can act as a structural material to which you can attach different payloads, such as drugs. In nature, this protein isn't close to being a structural material," said Tomasz Kowalewski, professor of chemistry in Carnegie Mellon's Mellon College of Science.

The building blocks of the fibers are a few modified green fluorescent protein (GFP) molecules linked together using a process called click chemistry. An ordinary GFP molecule does not normally bind with other GFP molecules to form fibers. But when Carnegie Mellon graduate student Saadyah Averick, working under the guidance of Krzysztof Matyjaszewski, the J.C. Warner Professor of Natural Sciences and University Professor of Chemistry in CMU's Mellon College of Science, modified the GFP molecules and attached PEO-dialkyne linkers to them, they noticed something strange the GFP molecules appeared to self-assemble into long fibers. Importantly, the fibers disassembled after being exposed to sound waves, and then reassembled within a few days. Systems that exhibit this type of reversible fibrous self-assembly have been long sought by scientists for use in applications such as tissue engineering, drug delivery, nanoreactors and imaging.

"This was purely curiosity-driven and serendipity-driven work," Kowalewski said. "But where controlled polymerization and organic chemistry meet biology, interesting things can happen."

The research team observed the fibers using confocal light microscopy, confirmed their assembly using dynamic light scattering and studied their morphology using atomic force microscopy (AFM). They also observed that the fibers were fluorescent, indicating that the GFP molecules retained their 3-D structure while linked together.

To determine what processes were driving the self-assembly, Matyjaszewski and Kowalewski turned to Anna Balazs, Distinguished Professor of Chemical Engineering and the Robert v. d. Luft Professor at the University of Pittsburgh. A leading expert in modeling the dynamics and mechanical properties of mesoscale systems, Balazs ran a computer simulation of the GFP molecules' self-assembly process using a technique called dissipative particle dynamics, a type of coarse-grained molecular dynamics method. The simulation confirmed the modified GFP's tendency to form fibers and revealed that the self-assembly process was driven by the interaction of hydrophobic patches on the surfaces of individual GFP molecules. In addition, Balazs's simulated fibers closely corresponded with what Kowalewski observed using AFM imaging.

"Our protein-polymer system gives us an atomically precise, very well-defined nanoscale building object onto which we can attach different handles in very precisely defined positions. It can be used in a way that wasn't ever intended by biology," Kowalewski said.

In addition to Averick, Balazs, Kowalewski and Matyjaszewski, co-authors of the study include Carnegie Mellon's Orsolya Karacsony and Jacob Mohin, University of Pittsburgh's Xin Yong and Nicholas M. Moellers, Oregon State University's Bradley F. Woodman and Ryan A. Mehl, and Zhejiang University's Weipu Zhu. The research was supported by the U.S. Department of Energy, National Science Foundation, Carnegie Mellon's CRP Consortium and Oregon State University.

####

For more information, please click here

Contacts:
Jocelyn Duffy

412-268-9982

Copyright © Carnegie Mellon University

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Iranian Researchers Planning to Produce Edible Insulin January 28th, 2015

Nanoparticles that deliver oligonucleotide drugs into cells described in Nucleic Acid Therapeutics January 28th, 2015

'Bulletproof' battery: Kevlar membrane for safer, thinner lithium rechargeables January 28th, 2015

Spider electro-combs its sticky nano-filaments January 28th, 2015

Imaging

JPK opens new expanded offices in Berlin to meet the growing demand for products worldwide January 28th, 2015

Pittcon News: Renishaw adds to the comprehensive imaging options available with its inVia confocal Raman microscope January 27th, 2015

Visualizing interacting electrons in a molecule: Scientists at Aalto University and the University of Zurich have succeeded in directly imaging how electrons interact within a single molecule January 26th, 2015

Graphene brings quantum effects to electronic circuits January 22nd, 2015

Synthetic Biology

New tool could help reshape the limits of synthetic biology: The 'telomerator' reshapes synthetic yeast chromosome into more flexible, realistic form, redefining what geneticists can build November 3rd, 2014

Tiny carbon nanotube pores make big impact October 29th, 2014

Smallest world record has 'endless possibilities' for bio-nanotechnology October 8th, 2014

Artificial Cells Act Like the Real Thing: Cell-like compartments produce proteins and communicate with one another, similar to natural biological systems August 18th, 2014

Govt.-Legislation/Regulation/Funding/Policy

Nanoscale Mirrored Cavities Amplify, Connect Quantum Memories: Advance could lead to quantum computing and the secure transfer of information over long-distance fiber optic networks January 28th, 2015

Detecting chemical weapons with a color-changing film January 28th, 2015

'Bulletproof' battery: Kevlar membrane for safer, thinner lithium rechargeables January 28th, 2015

Researchers Make Magnetic Graphene: UC Riverside research could lead to new multi-functional electronic devices January 27th, 2015

Self Assembly

Engineering self-assembling amyloid fibers January 26th, 2015

Revealed: How bacteria drill into our cells and kill them December 2nd, 2014

Live Images from the Nano-cosmos: Researchers watch layers of football molecules grow November 5th, 2014

Outsmarting Thermodynamics in Self-assembly of Nanostructures: Berkeley Lab reports method for symmetry-breaking in feedback-driven self-assembly of optical metamaterials November 4th, 2014

Discoveries

Detecting chemical weapons with a color-changing film January 28th, 2015

Joint international research project leads to a breakthrough in terahertz spectroscopy January 28th, 2015

Iranian Researchers Planning to Produce Edible Insulin January 28th, 2015

Nanoparticles that deliver oligonucleotide drugs into cells described in Nucleic Acid Therapeutics January 28th, 2015

Materials/Metamaterials

Spider electro-combs its sticky nano-filaments January 28th, 2015

Industrial Nanotech, Inc. Announces New OEM Customer January 27th, 2015

Chromium-centered cycloparaphenylene rings for making functionalized nanocarbons January 26th, 2015

Engineering self-assembling amyloid fibers January 26th, 2015

Announcements

Iranian Researchers Planning to Produce Edible Insulin January 28th, 2015

Nanoparticles that deliver oligonucleotide drugs into cells described in Nucleic Acid Therapeutics January 28th, 2015

'Bulletproof' battery: Kevlar membrane for safer, thinner lithium rechargeables January 28th, 2015

Spider electro-combs its sticky nano-filaments January 28th, 2015

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

Detecting chemical weapons with a color-changing film January 28th, 2015

Joint international research project leads to a breakthrough in terahertz spectroscopy January 28th, 2015

Nanoparticles that deliver oligonucleotide drugs into cells described in Nucleic Acid Therapeutics January 28th, 2015

'Bulletproof' battery: Kevlar membrane for safer, thinner lithium rechargeables January 28th, 2015

Tools

Joint international research project leads to a breakthrough in terahertz spectroscopy January 28th, 2015

JPK opens new expanded offices in Berlin to meet the growing demand for products worldwide January 28th, 2015

Pittcon News: Renishaw adds to the comprehensive imaging options available with its inVia confocal Raman microscope January 27th, 2015

Nanometrics to Present at the Stifel 2015 Technology, Internet and Media Conference January 27th, 2015

Research partnerships

Carbon nanoballs can greatly contribute to sustainable energy supply January 27th, 2015

Visualizing interacting electrons in a molecule: Scientists at Aalto University and the University of Zurich have succeeded in directly imaging how electrons interact within a single molecule January 26th, 2015

Promising use of nanodiamonds in delivering cancer drug to kill cancer stem cells: NUS study shows that delivery of Epirubicin by nanodiamonds resulted in a normally lethal dosage of Epirubicin becoming a safe and effective dosage for treatment of liver cancer January 26th, 2015

Wearable sensor clears path to long-term EKG, EMG monitoring January 20th, 2015

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2015 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE