Nanotechnology Now







Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Luminescent nanocrystal tags and high-speed scanner enable rapid detection of multiple pathogens in a single test

J. Paul Robinson
J. Paul Robinson

Abstract:
On-the-fly Decoding Luminescence Lifetimes in the μs Region for Lanthanide-Encoded Suspension Arrays

Yiqing Lu, Jie Lu, Jiangbo Zhao, Janet Cusido, Francisco M. Raymo, Jingli Yuan, Sean Yang, Robert C. Leif, Yujing Huo, James A. Piper, J. Paul Robinson, Ewa M. Goldys, and Dayong Jin

Significant multiplexing capacity of optical time-domain coding has been recently demonstrated by tuning luminescence lifetimes of the upconversion nanoparticles called "τ-Dots". It provides a large dynamic range of lifetimes from microseconds to milliseconds, which allows creating large libraries of nanotags/microcarriers. However a robust approach is required to rapidly and accurately measure the luminescence lifetimes from the relatively slow-decaying signals. Here, we show a fast algorithm suitable for the μs region with precision closely approaching the theoretical limit and compatible with the rapid scanning cytometry technique. We exploited this approach to further extend optical time-domain multiplexing to the down-conversion luminescence, using luminescence microspheres wherein lifetimes were tuned through Luminescence Resonance Energy Transfer (LRET). We demonstrated real-time discrimination of the LRET microspheres in the rapid scanning cytometry, and applied them to the multiplexed probing of pathogen DNA strands. Our results indicate that tunable luminescence lifetimes have considerable potential in high-throughput analytical sciences.

DOI: 10.1038/ncomms4741

Luminescent nanocrystal tags and high-speed scanner enable rapid detection of multiple pathogens in a single test

West Lafayette, IN | Posted on May 8th, 2014

A research team using tunable luminescent nanocrystals as tags to advance medical and security imaging have successfully applied them to high-speed scanning technology and detected multiple viruses within minutes.

The research, led by Macquarie University in Sydney, Australia and Purdue University, builds on the team's earlier success in developing a way to control the length of time light from a luminescent nanocrystal lingers, which introduced the dimension of time in addition to color and brightness in optical detection technology.

Detection based on the lifetime of the light from a nanocrystal as well as its specific color exponentially increases the possible combinations and unique tags that could be created for biomedical screens.

"We now are able to build a huge library of lifetime color-coded microspheres to perform multiple medical tasks or diagnoses at the same time," said Yiqing Lu, a researcher at Macquarie University, who led the research. "The time saved by omitting the need to grow or amplify a culture sample for testing and eliminating the need to run multiple tests will save future patients precious time so treatment can begin, which can be life-saving when managing aggressive diseases."

The technology could enable screens that identify thousands of different target molecules simultaneously, said J. Paul Robinson, the Professor of Cytomics in Purdue's College of Veterinary Medicine and professor in Purdue's Weldon School of Biomedical Engineering, who was involved in the research.

"This is the second part of the puzzle," said Robinson, who led the biological testing of the technology. "Now we've successfully measured the lifetimes of these tags on the fly at thousands of samples per second. The next step is to perform such high-throughput testing within a liquid, like water, blood or urine. That will open the door to widespread biological use and clinical applications, as well as the detection of pathogens in food or water."

Robinson's research focuses on flow cytometry, the analysis of cells that are contained in a liquid flowing past a laser beam. In addition to developing instrumentation to measure the tags, he plans to explore the technology's health care and biodetection applications.

The research team attached unique tags to DNA strands of HIV, Ebola virus, Hepatitis B virus and Human Papillomavirus 16. The tags were accurately read and distinguished at high speeds in suspension arrays. The team's work is detailed in a paper that will be published in the next issue of Nature Communications and is currently available online.

Dayong Jin, an Australian Research Council Future Fellow, and a professor of photonics at Macquarie ARC Centre for nanoscale BioPhotonics (CNBP), led the design and manufacture of the nanoparticles, which the researchers named tau-dots.

In addition to Jin, Lu and Robinson, paper co-authors include Jie Lu, Jiangbo Zhao, Ewa M. Goldys, and James A. Piper of Macquarie; Janet Cusido and Francisco M. Raymo of the University of Miami; Jingli Yuan of Dalian University of Technology in Dalian, China; , Sean Yang and Robert C. Leif of Newport Instruments in San Diego; and Yujing Huo of Tsinghua Univesity in Beijing, China.

The Australian Research Council funded this work.

####

For more information, please click here

Contacts:
Writer:
Elizabeth K. Gardner

765-494-2081

Media contact for Macquarie University:
Amy Macintyre
02-9850-4051


Sources:
J. Paul Robinson
765-494-0757


Dayong Jin
+61 2 98504168


Yiqing Lu
+61 2 98504169

Copyright © Purdue University

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

Purdue University Cytometry Laboratories:

J. Paul Robinson web page:

Related News Press

News and information

The George Washington University Opens Science and Engineering Hall, Largest Building of Its Kind in D.C.: Building Represents Significant Investment in Research Programs and Facilities; Commitment to Solve Global Problems, Improve Lives of Millions March 5th, 2015

Anousheh Ansari Wins the National Space Society's Space Pioneer Award for "Service to the Space Community" March 5th, 2015

Enhanced Graphene Components for Next Generation Racing Yacht March 5th, 2015

Get ready for NanoDays! March 5th, 2015

Imaging

Forbidden quantum leaps possible with high-res spectroscopy March 2nd, 2015

International research partnership tricks the light fantastic March 2nd, 2015

Important step towards quantum computing: Metals at atomic scale March 2nd, 2015

Real-time observation of bond formation by using femtosecond X-ray liquidography February 26th, 2015

Govt.-Legislation/Regulation/Funding/Policy

New research could lead to more efficient electrical energy storage March 4th, 2015

Energy-generating cloth could replace batteries in wearable devices March 4th, 2015

The taming of magnetic vortices: Unified theory for skyrmion-materials March 3rd, 2015

Black phosphorus is new 'wonder material' for improving optical communication March 3rd, 2015

Nanomedicine

Patent for the Novel Cancer Therapies – Ceramide Nanoliposomes March 4th, 2015

Arrowhead to Present at 2015 Barclays Global Healthcare Conference March 4th, 2015

Democratizing synthetic biology: New method makes research cheaper, faster, and more accessible March 3rd, 2015

Pens filled with high-tech inks for do-it-yourself sensors March 3rd, 2015

Discoveries

Enhanced Graphene Components for Next Generation Racing Yacht March 5th, 2015

American Chemical Society Presidential Symposia: nanoscience, international chemistry March 5th, 2015

Magnetic vortices in nanodisks reveal information: Researchers from Dresden and Jülich use microwaves to read out information from smallest storage devices March 4th, 2015

CiQUS researchers obtain high-quality perovskites over large areas by a chemical method March 4th, 2015

Announcements

The George Washington University Opens Science and Engineering Hall, Largest Building of Its Kind in D.C.: Building Represents Significant Investment in Research Programs and Facilities; Commitment to Solve Global Problems, Improve Lives of Millions March 5th, 2015

Anousheh Ansari Wins the National Space Society's Space Pioneer Award for "Service to the Space Community" March 5th, 2015

Enhanced Graphene Components for Next Generation Racing Yacht March 5th, 2015

Get ready for NanoDays! March 5th, 2015

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

Strength in numbers: Researchers develop the first-ever quantum device that detects and corrects its own errors March 4th, 2015

Energy-generating cloth could replace batteries in wearable devices March 4th, 2015

Experiment and theory unite at last in debate over microbial nanowires: New model and experiments settle debate over metallic-like conductivity of microbial nanowires in bacterium March 4th, 2015

Magnetic vortices in nanodisks reveal information: Researchers from Dresden and Jülich use microwaves to read out information from smallest storage devices March 4th, 2015

Tools

Keysight Technologies Shifts to Direct Sales of High-Performance Products in North America March 3rd, 2015

Forbidden quantum leaps possible with high-res spectroscopy March 2nd, 2015

International research partnership tricks the light fantastic March 2nd, 2015

Important step towards quantum computing: Metals at atomic scale March 2nd, 2015

Research partnerships

French Institutes IRT Nanoelec and CMP Team up to Offer World’s First Service for Post-process 3D Technologies on Multi-Project-Wafer March 5th, 2015

New research could lead to more efficient electrical energy storage March 4th, 2015

Cambrios and Heraeus Jointly Create New, High-Conductivity Transparent Conductors: Two Companies' Combined Products Dramatically Extend Flexible Substrate Capabilities for Next-Generation Mass-Market Technology Products March 3rd, 2015

The taming of magnetic vortices: Unified theory for skyrmion-materials March 3rd, 2015

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2015 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE