Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > Inhibition of oral biofilm and cell-cell communication using natural-products derivatives

Abstract:
Today during the 43rd Annual Meeting & Exhibition of the American Association for Dental Research, held in conjunction with the 38th Annual Meeting of the Canadian Association for Dental Research, Steve Kasper, SUNY College of Nanoscale Science and Engineering, Albany, will present research titled "Inhibition of Oral Biofilm and Cell-cell Communication Using Natural-products Derivatives."

Inhibition of oral biofilm and cell-cell communication using natural-products derivatives

Alexandria, VA | Posted on March 20th, 2014

Many plant metabolites and structurally similar derivatives have been identified as inhibitors of bacterial biofilm formation and quorum sensing (QS). Previously, the researchers of this study demonstrated biofilm and QS inhibition using modified cysteines, similar to those produced by the tropical plant Petiveria alliacea. In this study the researchers expanded their compound library to examine structure-activity relationships for biofilm and QS inhibition.

Using a microplate-based screening approach, they observed the biofilm formation by three indigenous oral Gram-positive bacteria: Streptococcus mutans UA159, Streptococcus sanguis 10556, and Actinomyces oris MG1. Bacteria were grown in the presence of inhibitory compounds and analyzed using fluorescent staining for biomass and via confocal microscopy.

Compounds were also tested in a Vibrio harveyi QS reporter which responds to autoinducer-2 (AI-2) signaling (interspecies) but not acyl-homoserine lactone signaling (intraspecies). Reverse transcriptase real-time PCR and global RNA sequencing (RNAseq) were used to study modified genetic expression in S. mutans UA159 in the presence of select compounds from our library.

From their 46 compound library, six were capable of inhibiting biofilm formation in all three species tested at a concentration of 1mM. All six compounds are structurally similar to S-ribosyl homocysteine, the precursor for autoinducer-2 biosynthesis. These compounds also reduced bioluminescence in V. harveyi BB170, indicating inhibition of AI-2 based QS. Gene expression analysis showed distinct down-regulation of genes previously related to quorum sensing and/or biofilm formation in S. mutans.

The use of plant-inspired cysteine derivatives to inhibit bacterial virulence may serve as a novel tool to improve oral health. The researchers of this study propose that the compounds used in this study may inhibit biofilm formation by interrupting bacterial communication pathways, particularly in AI-2 biosynthetic reactions. Since their library is derived from eukaryotic (plant) origins, this study may provide initial evidence of interkingdom signaling, which has implications for studies of the human microbiome.

###

This research was supported by NIH NIDCR grant # 5R03DE020834-02.

This is a summary of abstract #362, "Inhibition of Oral Biofilm and Cell-cell Communication Using Natural-products Derivatives," which will be presented on Thursday, March 20, 2014: 2 p.m. - 3:15 p.m. in Exhibit Hall AB of the Charlotte Convention Center.

####

About International & American Associations for Dental Research
The American Association for Dental Research (AADR), headquartered in Alexandria, Va., is a nonprofit organization with more than 3,600 members in the United States. Its mission is: (1) to advance research and increase knowledge for the improvement of oral health; (2) to support and represent the oral health research community; and (3) to facilitate the communication and application of research findings. AADR is the largest Division of the International Association for Dental Research (IADR). To learn more about the AADR, visit www.aadr.org.

For more information, please click here

Contacts:
Ingrid L. Thomas

703-299-8084

Copyright © International & American Associations for Dental Research

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Basque researchers turn light upside down February 23rd, 2018

Stiffness matters February 23rd, 2018

Imaging individual flexible DNA 'building blocks' in 3-D: Berkeley Lab researchers generate first images of 129 DNA structures February 22nd, 2018

'Memtransistor' brings world closer to brain-like computing: Combined memristor and transistor can process information and store memory with one device February 22nd, 2018

Discoveries

Basque researchers turn light upside down February 23rd, 2018

Histology in 3-D: New staining method enables Nano-CT imaging of tissue samples February 22nd, 2018

Developing reliable quantum computers February 22nd, 2018

Imaging individual flexible DNA 'building blocks' in 3-D: Berkeley Lab researchers generate first images of 129 DNA structures February 22nd, 2018

Announcements

Basque researchers turn light upside down February 23rd, 2018

Stiffness matters February 23rd, 2018

Histology in 3-D: New staining method enables Nano-CT imaging of tissue samples February 22nd, 2018

Developing reliable quantum computers February 22nd, 2018

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

Basque researchers turn light upside down February 23rd, 2018

Stiffness matters February 23rd, 2018

Histology in 3-D: New staining method enables Nano-CT imaging of tissue samples February 22nd, 2018

Developing reliable quantum computers February 22nd, 2018

Events/Classes

European & Korean Project To Demo World’s First 5G Platform During Winter Games February 15th, 2018

Leti’s Chief Scientist Presents Optimistic Vision for Neuromorphic Hardware and Ultra-Low-Power Microdevices for Edge Computing at ISSCC: Leti’s Chief Scientist Presents Optimistic Vision for Neuromorphic Hardware and Ultra-Low-Power Microdevices That Are Based on Novel Emerging February 13th, 2018

Leti Chief Scientist Barbara De Salvo Will Help Kick Off ISSCC 2018 with Opening-Day Keynote: In Addition, Leti Scientists Will Present and Demo New Technology for Piezoelectric Energy Harvesting February 8th, 2018

Leti Presents Optical-Equipment Curving Technology that Improves Performance, Cuts Costs: ‘Disruptive Approach’ for Imaging Applications Presented in Paper At Photonics West and Demonstrated in Leti’s Booth February 2nd, 2018

Dental

A Tougher Tooth: A new dental restoration composite developed by UCSB scientists proves more durable than the conventional material August 22nd, 2017

New technology can detect tiny ovarian tumors: 'Synthetic biomarkers' could be used to diagnose ovarian cancer months earlier than now possible April 10th, 2017

New stem cell technique shows promise for bone repair January 25th, 2017

Nanocellulose in medicine and green manufacturing: American University professor develops method to improve performance of cellulose nanocrystals November 7th, 2016

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project