Nanotechnology Now





Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > St George's Hospital uses Nanoparticle Tracking Analysis to study extracellular vesicles as biomarkers for diseases

Michelle Levene, a PhD student of the Clinical Sciences Division at St Georges uses the NanoSight LM10 NTA system used to characterize proteins and peptides.
Michelle Levene, a PhD student of the Clinical Sciences Division at St Georges uses the NanoSight LM10 NTA system used to characterize proteins and peptides.

Abstract:
NanoSight reports on how Nanoparticle Tracking Analysis, NTA, is being used at St George's, University of London, to explore extracellular vesicles as a potential source of biomarkers, by identifying proteins or peptides differentially expressed between healthy subjects and patients with rare inherited diseases

St George's Hospital uses Nanoparticle Tracking Analysis to study extracellular vesicles as biomarkers for diseases

Salisbury, UK | Posted on August 13th, 2013

St George's, University of London, is the UK's only independent medical and healthcare higher education institution. Dr Bridget Bax is a senior research fellow in the Clinical Sciences Division where the main focus of her group's research is to improve the understanding of the pathogenic mechanisms of rare inherited diseases and to develop novel therapies for translation into the clinical setting. One of their major areas of interest is the identification and validation of biomarkers in patients with the rare and fatal disease mitochondrial neurogastrointestinal encephalomyopathy (MNGIE). They are currently exploring the use of extracellular vesicles as source of biomarkers by identifying proteins or peptides differentially expressed between healthy subjects and patients with MNGIE. The goals of this work are three-fold: to understand the pathophysiological mechanisms and metabolic derangements observed in patients with MNGIE; to provide a means of monitoring more effectively clinical and biochemical response to therapy; and to enable the tracking of disease progression in diagnosed patients.

Describing her work, Dr Bax said "We have used several methods in the lab to isolate both exosomes and microparticles. We needed a reproducible method that would allow us to i) quantitate and size the extracellular vesicles we isolated and ii) determine whether the isolation technique employed affected these parameters. Nanoparticle Tracking Analysis has allowed us to quantify extracellular vesicles with diameters in the range of 50 to 1000 nm. This was particularly important for us because we specifically wanted to study exosomes which have a diameter ranging from 40 to 100 nm."

Prior to using NTA, initially the group used electron microscopy and fluorescence-activated cell sorting (FACS), a biophysical technique used in flow cytometry. Using FACS, Dr Bax said "We were unable to detect exosomes and found that a significant number of microparticles were missed due to not detecting vesicles with a diameter <300 nm. Although it was possible to size extracellular vesicles using electron microscopy, this is a time intensive technique and has the potential disadvantages of causing shrinkage."

Dr Bax went on to discuss her thoughts on using NTA: "The benefits are the ability to detect particles within the size range of interest. NTA uses small sample volumes which is extremely important in terms of the rare diseases we study. We find the system easy to use."

To find out about the company and to learn more about particle characterization using NanoSight's unique Nanoparticle Tracking Analysis solutions, visit www.nanosight.com and register to receive the next issue of NanoTrail, the company's electronic newsletter.

####

About NanoSight
NanoSight delivers the world's most versatile and proven multi-parameter nanoparticle analysis in a single instrument.

NanoSight's "Nanoparticle Tracking Analysis" (NTA) detects and visualizes populations of nanoparticles in liquids down to 10 nm, dependent on material, and measures the size of each particle from direct observations of diffusion. Additionally, NanoSight measures concentration and a fluorescence mode differentiates suitably-labelled particles within complex background suspensions. Zeta potential measurements are similarly particle-specific. It is this particle-by-particle methodology that takes NTA beyond traditional light scattering and other ensemble techniques in providing high-resolution particle size distributions and validates data with information-rich video files of the particles moving under Brownian motion.

This simultaneous multiparameter characterization matches the demands of complex biological systems, hence its wide application in development of drug delivery systems, of viral vaccines, and in nanotoxicology. This real-time data gives insight into the kinetics of protein aggregation and other time-dependent phenomena in a qualitative and quantitative manner. NanoSight has a growing role in biodiagnostics, being proven in detection and speciation of nanovesicles (exosomes) and microvesicles.

NanoSight has installed more than 600 systems worldwide with users including BASF, GlaxoSmithKline, Merck, Novartis, Pfizer, Proctor and Gamble, Roche and Unilever together with the most eminent universities and research institutes. NanoSight's technology is validated by 850+ third party papers citing NanoSight results. NanoSight's leadership position in nanoparticle characterization is consolidated further with publication of an ASTM International standard, ASTM E2834, which describes the NTA methodology for detection and analysis of nanoparticles.

For more information, please click here

Contacts:
NanoSight Limited
Minton Park
London Road
Amesbury SP4 7RT UK
T +44(0)1980 676060
F +44(0)1980 624703
www.nanosight.com


Talking Science Limited
39 de Bohun Court
Saffron Walden
Essex CB10 2BA UK
T +44(0)1799 521881
M +44(0)7843 012997
www.talking-science.com

Copyright © NanoSight

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Tel Aviv/Tsinghua University project uses crowd computing to improve water filtration: The research, a product of the new TAU-Tsinghua XIN Center, was conducted by 150,000 volunteers at IBM's World Community Grid July 6th, 2015

Transition from 3 to 2 dimensions increases conduction, MIPT scientists discover July 6th, 2015

A Stretchy Mesh Heater for Sore Muscles July 6th, 2015

BBC World Service to broadcast Forum discussion on graphene July 6th, 2015

Imaging

A 'movie' of ultrafast rotating molecules at a hundred billion per second: A quantum wave-like nature was successfully observed in rotating nitrogen molecules July 4th, 2015

Clues to inner atomic life from subtle light-emission shifts: Hyperfine structure of light absorption by short-lived cadmium atom isotopes reveals characteristics of the nucleus that matter for high precision detection methods July 3rd, 2015

NIST ‘How-To’ Website Documents Procedures for Nano-EHS Research and Testing July 1st, 2015

Ultra-stable JILA microscopy technique tracks tiny objects for hours July 1st, 2015

Nanomedicine

A Stretchy Mesh Heater for Sore Muscles July 6th, 2015

New Biosensor Produced in Iran to Detect Effective Drugs in Cancer Treatment July 4th, 2015

Groundbreaking research to help control liquids at micro and nano scales July 3rd, 2015

Iranian Scientists Find Simple, Economic Method to Synthesize Antibacterial Nanoparticles July 2nd, 2015

Discoveries

Transition from 3 to 2 dimensions increases conduction, MIPT scientists discover July 6th, 2015

A Stretchy Mesh Heater for Sore Muscles July 6th, 2015

Production of Zirconium Carbide Nanoparticles at Low Temperature without Thermal Operations July 5th, 2015

A 'movie' of ultrafast rotating molecules at a hundred billion per second: A quantum wave-like nature was successfully observed in rotating nitrogen molecules July 4th, 2015

Announcements

Tel Aviv/Tsinghua University project uses crowd computing to improve water filtration: The research, a product of the new TAU-Tsinghua XIN Center, was conducted by 150,000 volunteers at IBM's World Community Grid July 6th, 2015

Transition from 3 to 2 dimensions increases conduction, MIPT scientists discover July 6th, 2015

A Stretchy Mesh Heater for Sore Muscles July 6th, 2015

BBC World Service to broadcast Forum discussion on graphene July 6th, 2015

Tools

A 'movie' of ultrafast rotating molecules at a hundred billion per second: A quantum wave-like nature was successfully observed in rotating nitrogen molecules July 4th, 2015

Clues to inner atomic life from subtle light-emission shifts: Hyperfine structure of light absorption by short-lived cadmium atom isotopes reveals characteristics of the nucleus that matter for high precision detection methods July 3rd, 2015

Nanometrics to Announce Second Quarter Financial Results on July 23, 2015 July 2nd, 2015

Ultra-stable JILA microscopy technique tracks tiny objects for hours July 1st, 2015

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project