Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Temp-controlled 'nanopores' may allow detailed blood analysis

By tethering gold nanoparticles (large spheres in top image) to the nanopore (violet), the temperature around the nanopore can be changed quickly and precisely with laser light, allowing scientists to distinguish between similar molecules in the pore that behave differently under varied temperature conditions.

Credit: Robertson/NIST
By tethering gold nanoparticles (large spheres in top image) to the nanopore (violet), the temperature around the nanopore can be changed quickly and precisely with laser light, allowing scientists to distinguish between similar molecules in the pore that behave differently under varied temperature conditions.

Credit: Robertson/NIST

Abstract:
Tiny biomolecular chambers called nanopores that can be selectively heated may help doctors diagnose disease more effectively if recent research by a team at the National Institute of Standards and Technology (NIST), Wheaton College, and Virginia Commonwealth University (VCU) proves effective. Though the findings* may be years away from application in the clinic, they may one day improve doctors' ability to search the bloodstream quickly for indicators of disease—a longstanding goal of medical research.

Temp-controlled 'nanopores' may allow detailed blood analysis

Gaithersburg, MD | Posted on March 9th, 2013

The team has pioneered work on the use of nanopores—tiny chambers that mimic the ion channels in the membranes of cells—for the detection and identification of a wide range of molecules, including DNA. Ion channels are the gateways by which the cell admits and expels materials like proteins, ions and nucleic acids. The typical ion channel is so small that only one molecule can fit inside at a time.

Previously, team members inserted a nanopore into an artificial cell membrane, which they placed between two electrodes. With this setup, they could drive individual molecules into the nanopore and trap them there for a few milliseconds, enough to explore some of their physical characteristics.

"A single molecule creates a marked change in current that flows through the pore, which allows us to measure the molecule's mass and electrical charge with high accuracy," says Joseph Reiner, a physicist at VCU who previously worked at NIST. "This enables discrimination between different molecules at high resolution. But for real-world medical work, doctors and clinicians will need even more advanced measurement capability."

A goal of the team's work is to differentiate among not just several types of molecules, but among the many thousands of different proteins and other biomarkers in our bloodstream. For example, changes in protein levels can indicate the onset of disease, but with so many similar molecules in the mix, it is important not to mistake one for another. So the team expanded their measurement capability by attaching gold nanoparticles to engineered nanopores, "which provides another means to discriminate between various molecular species via temperature control," Reiner says.

The team attached gold nanoparticles to the nanopore via tethers made from complementary DNA strands. Gold's ability to absorb light and quickly convert its energy to heat that conducts into the adjacent solution allows the team to alter the temperature of the nanopore with a laser at will, dynamically changing the way individual molecules interact with it.

"Historically, sudden temperature changes were used to determine the rates of chemical reactions that were previously inaccessible to measurement," says NIST biophysicist John Kasianowicz. "The ability to rapidly change temperatures in volumes commensurate with the size of single molecules will permit the separation of subtly different species. This will not only aid the detection and identification of biomarkers, it will also help develop a deeper understanding of thermodynamic and kinetic processes in single molecules."

The team is researching ways to improve semiconductor-based nanopores, which could further expand this new measurement capability.

*J.E. Reiner, J.W.F. Robertson, D.L. Burden, L.K. Burden, A. Balijepalli and J.J. Kasianowicz. Temperature sculpting in yoctoliter volumes. Journal of the American Chemical Society, DOI: 10.1021/ja309892e. Jan. 24, 2013.

####

For more information, please click here

Contacts:
Chad Boutin

301-975-4261

Copyright © National Institute of Standards and Technology (NIST)

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Measuring the Smallest Magnets July 28th, 2014

WITec to host the 11th Confocal Raman Imaging Symposium from September 29th - October 1st in Ulm, Germany July 28th, 2014

FEI adds Phase Plate Technology and Titan Halo TEM to its Structural Biology Product Portfolio: New solutions provide the high-quality imaging and contrast necessary to analyze the 3D structure of molecules and molecular complexes July 28th, 2014

Production of Toxic Gas Sensor Based on Nanorods July 28th, 2014

Laboratories

Stanford team achieves 'holy grail' of battery design: A stable lithium anode - Engineers use carbon nanospheres to protect lithium from the reactive and expansive problems that have restricted its use as an anode July 27th, 2014

NIST shows ultrasonically propelled nanorods spin dizzyingly fast July 22nd, 2014

Sono-Tek Corporation Announces New Clean Room Rated Laboratory Facility in China July 18th, 2014

Fundamental Chemistry Findings Could Help Extend Moore’s Law: A Berkeley Lab-Intel collaboration outlines the chemistry of photoresist, enabling smaller features for future generations of microprocessors July 15th, 2014

Lab-on-a-chip

EPFL Scientists use nanoscale IR spectroscopy to demonstrate α to β secondary structure transition associated with amyloid formation June 10th, 2014

Fully automated DNA lab-on-a-chip microfluidic system wins Dolomite’s Productizing Science® competition 2013 June 10th, 2014

One small chip -- one giant leap forward for early cancer detection: An ultra-sensitive nano-chip capable of detecting cancer at early stages May 19th, 2014

A Lab in Your Pocket May 7th, 2014

Govt.-Legislation/Regulation/Funding/Policy

Seeing is bead-lieving: Rice University scientists create model 'bead-spring' chains with tunable properties July 28th, 2014

Stanford team achieves 'holy grail' of battery design: A stable lithium anode - Engineers use carbon nanospheres to protect lithium from the reactive and expansive problems that have restricted its use as an anode July 27th, 2014

New imaging agent provides better picture of the gut July 25th, 2014

A*STAR and industry form S$200M semiconductor R&D July 25th, 2014

Nanomedicine

FEI adds Phase Plate Technology and Titan Halo TEM to its Structural Biology Product Portfolio: New solutions provide the high-quality imaging and contrast necessary to analyze the 3D structure of molecules and molecular complexes July 28th, 2014

New imaging agent provides better picture of the gut July 25th, 2014

Scientists Test Nanoparticle "Alarm Clock" to Awaken Immune Systems Put to Sleep by Cancer July 25th, 2014

Researchers create vaccine for dust-mite allergies Main Page Content: Vaccine reduced lung inflammation to allergens in lab and animal tests July 22nd, 2014

Discoveries

Seeing is bead-lieving: Rice University scientists create model 'bead-spring' chains with tunable properties July 28th, 2014

Measuring the Smallest Magnets July 28th, 2014

Production of Toxic Gas Sensor Based on Nanorods July 28th, 2014

Stanford team achieves 'holy grail' of battery design: A stable lithium anode - Engineers use carbon nanospheres to protect lithium from the reactive and expansive problems that have restricted its use as an anode July 27th, 2014

Announcements

Measuring the Smallest Magnets July 28th, 2014

WITec to host the 11th Confocal Raman Imaging Symposium from September 29th - October 1st in Ulm, Germany July 28th, 2014

FEI adds Phase Plate Technology and Titan Halo TEM to its Structural Biology Product Portfolio: New solutions provide the high-quality imaging and contrast necessary to analyze the 3D structure of molecules and molecular complexes July 28th, 2014

Production of Toxic Gas Sensor Based on Nanorods July 28th, 2014

Research partnerships

Breakthrough laser experiment reveals liquid-like motion of atoms in an ultra-cold cluster: University of Leicester research team unlocks insights into creation of new nano-materials July 25th, 2014

A*STAR and industry form S$200M semiconductor R&D July 25th, 2014

A Crystal Wedding in the Nanocosmos July 23rd, 2014

Penn Study: Understanding Graphene’s Electrical Properties on an Atomic Level July 22nd, 2014

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE