Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > New biochip technology uses tiny whirlpools to corral microbes

Researchers have demonstrated a new technology that combines a laser and electric fields to create tiny centrifuge-like whirlpools to separate particles and microbes by size, a potential lab-on-a-chip system for medicine and research. Here the technique is used to collect a bacterium called Shewanella oneidensis. (Purdue University image)
Researchers have demonstrated a new technology that combines a laser and electric fields to create tiny centrifuge-like whirlpools to separate particles and microbes by size, a potential lab-on-a-chip system for medicine and research. Here the technique is used to collect a bacterium called Shewanella oneidensis.

(Purdue University image)

Abstract:
Opto-Electrokinetic Manipulation for High-Performance On-Chip Bioassays

Jae-Sung Kwon,a Sandeep P. Ravindranath,b Aloke Kumar,c Joseph Irudayarajb and Steven T. Wereley*a

aSchool of Mechanical Engineering and Birck Nanotechnology Center, Purdue University

bSchool of Agricultural and Biological Engineering and Bindley Bioscience Center, Purdue University

cBiosciences Division, Oak Ridge National Laboratory

This communication first demonstrates bio-compatibility of a recently developed opto-electrokinetic manipulation technique, using microorganisms. Aggregation, patterning, translation, trapping and size-based separation of microorganisms performed with the technique firmly establishes its usefulness for development of a high-performance on-chip bioassay process. Ultimately fast and precise on-chip manipulation of microorganisms aids in development of high-performance bioassay systems.

New biochip technology uses tiny whirlpools to corral microbes

West Lafayette, IN | Posted on January 8th, 2013

Researchers have demonstrated a new technology that combines a laser and electric fields to create tiny centrifuge-like whirlpools to separate particles and microbes by size, a potential lab-on-a-chip system for medicine and research.

The theory behind the technology, called rapid electrokinetic patterning - or REP - has been described in technical papers published between 2008 and 2011. Now the researchers have used the method for the first time to collect microscopic bacteria and fungi, said Steven T. Wereley, a Purdue University professor of mechanical engineering.

The technology could bring innovative sensors and analytical devices for lab-on-a-chip applications, or miniature instruments that perform measurements normally requiring large laboratory equipment. REP is a potential new tool for applications including medical diagnostics; testing food, water and contaminated soil; isolating DNA for gene sequencing; crime-scene forensics; and pharmaceutical manufacturing.

"The new results demonstrate that REP can be used to sort biological particles but also that the technique is a powerful tool for development of a high-performance on-chip bioassay system," Wereley said.

A research paper about the technology was featured on the cover of the Dec. 7 issue of Lab on a Chip magazine, and the work is highlighted as a news item in the Jan. 13 issue of Nature Photonics, posted online Dec. 27. Mechanical engineering doctoral student Jae-Sung Kwon, working extensively with Sandeep Ravindranath, a doctoral student in agricultural and biological engineering, was lead author of the Lab on a Chip paper.

The technology works by using a highly focused infrared laser to heat fluid in a microchannel containing particles or bacteria. An electric field is applied, combining with the laser's heating action to circulate the fluid in a "microfluidic vortex," whirling mini-maelstroms one-tenth the width of a human hair that work like a centrifuge to isolate specific types of particles based on size.

Particles of different sizes can be isolated by changing the electrical frequency, and the vortex moves wherever the laser is pointed, representing a method for positioning specific types of particles for detection and analysis.

The Lab on a Chip paper was written by Kwon; Ravindranath; Aloke Kumar, a researcher at the Oak Ridge National Laboratory; Joseph Irudayaraj, a Purdue professor of agricultural and biological engineering and deputy director of the Bindley Bioscience Center; and Wereley.

Much of the research has been based at the Birck Nanotechnology Center in Purdue's Discovery Park, in collaboration with Irudayaraj's group in the Bindley Bioscience Center.

The researchers used REP to collect three types of microorganisms: a bacterium called Shewanella oneidensis MR-1; Saccharomyces cerevisiae, a single-cell spherical fungus; and Staphylococcus aureus, a spherical bacterium. The new findings demonstrate the tool's ability to perform size-based separation of microorganisms, Wereley said.

"By properly choosing the electrical frequency we can separate blood components, such as platelets," he said. "Say you want to collect Shewanella bacteria, so you use a certain electrical frequency and collect them. Then the next day you want to collect platelets from blood. That's going to be a different frequency. We foresee the ability to dynamically select what you will collect, which you could not do with conventional tools."

The overall research field is called "optoelectrical microfluidics." More research is needed before the technology is ready for commercialization.

"It won't be on the market in a year," Wereley said. "We are still in the research end of this. We are sort of at the stage of looking for the killer app for this technology."

REP may be used as a tool for nanomanufacturing because it shows promise for the assembly of suspended particles, called colloids. The ability to construct objects with colloids makes it possible to create structures with particular mechanical and thermal characteristics to manufacture electronic devices and tiny mechanical parts.

Purdue researchers are pursuing the technology for pharmaceutical manufacturing, Wereley said, because a number of drugs are manufactured from solid particles suspended in liquid. The particles have to be collected and separated from the liquid. This process is now done using filters and centrifuges.

REP also might be used to diagnose the presence of viruses, as well, although it has not yet been used to separate viruses from a sample, Wereley said.

Unlike conventional tools, REP requires only tiny samples, making it potentially practical for medical diagnostics and laboratory analysis.

####

For more information, please click here

Contacts:
Writer:
Emil Venere
765-494-4709


Sources:
Steven T. Wereley
765-494-5624


Joseph Irudayaraj
765-494-0388


Aloke Kumar
865-574-8661

Copyright © Purdue University

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

Lab on a Chip on Twitter:

Related News Press

News and information

GLOBALFOUNDRIES and Soitec Enter Into Long-term Supply Agreement on FD-SOI Wafers: Strategic milestone to help guarantee a secure, high-volume supply of FD-SOI technology September 20th, 2017

GLOBALFOUNDRIES Announces Availability of mmWave and RF/Analog on Leading FDX™ FD-SOI Technology Platform: Technology solution delivers ‘connected intelligence’ to next generation high-volume wireless and IoT applications with lower power and significantly reduced cost September 20th, 2017

GLOBALFOUNDRIES Announces Availability of Embedded MRAM on Leading 22FDX® FD-SOI Platform: Advanced embedded non-volatile memory solution delivers ‘connected intelligence’ by expanding SoC capabilities on the 22nm process node September 20th, 2017

Copper catalyst yields high efficiency CO2-to-fuels conversion: Berkeley Lab scientists discover critical role of nanoparticle transformation September 20th, 2017

Lab-on-a-chip

Researchers make flexible glass for tiny medical devices: Glass can bend over and over again on a nanoscale March 27th, 2017

New research helps to meet the challenges of nanotechnology: Research helps to make the most of nanoscale catalytic effects for nanotechnology January 20th, 2017

Ultra-precise chip-scale sensor detects unprecedentedly small changes at the nanoscale January 20th, 2017

New graphene-based system could help us see electrical signaling in heart and nerve cells: Berkeley-Stanford team creates a system to visualize faint electric fields December 19th, 2016

Nanomedicine

Do titanium dioxide particles from orthopedic implants disrupt bone repair? September 16th, 2017

Arrowhead Hosts Investor & Analyst R&D Day to Introduce TRiM(TM) Platform and Lead RNAi-based Drug Candidates September 14th, 2017

Graphene based terahertz absorbers: Printable graphene inks enable ultrafast lasers in the terahertz range September 13th, 2017

Applications for the nanomedTAB are open until September 18th, 2017 September 13th, 2017

Discoveries

Copper catalyst yields high efficiency CO2-to-fuels conversion: Berkeley Lab scientists discover critical role of nanoparticle transformation September 20th, 2017

Solar-to-fuel system recycles CO2 to make ethanol and ethylene: Berkeley Lab advance is first demonstration of efficient, light-powered production of fuel via artificial photosynthesis September 19th, 2017

A new approach to ultrafast light pulses: Unusual fluorescent materials could be used for rapid light-based communications systems September 19th, 2017

New quantum phenomena in graphene superlattices September 18th, 2017

Announcements

GLOBALFOUNDRIES Delivers 8SW RF SOI Technology for Next-Generation Mobile and 5G Applications: Advanced 8SW 300mm SOI technology enables cost-effective, high-performance RF front-end modules for 4G LTE mobile and sub-6GHz 5G applications September 20th, 2017

GLOBALFOUNDRIES Unveils Vision and Roadmap for Next-Generation 5G Applications: Technology platforms are uniquely positioned to enable a new era of ‘connected intelligence’ with the transition to 5G September 20th, 2017

GLOBALFOUNDRIES Delivers Custom 14nm FinFET Technology for IBM Systems: Jointly developed 14HP process is world’s only technology that leverages both FinFET and SOI September 20th, 2017

GLOBALFOUNDRIES Introduces New 12nm FinFET Technology for High-Performance Applications September 20th, 2017

Nanobiotechnology

Do titanium dioxide particles from orthopedic implants disrupt bone repair? September 16th, 2017

Applications for the nanomedTAB are open until September 18th, 2017 September 13th, 2017

Magnetic cellular 'Legos' for the regenerative medicine of the future September 12th, 2017

How to draw electricity from the bloodstream: A one-dimensional fluidic nanogenerator with a high power-conversion efficiency September 11th, 2017

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project