Nanotechnology Now







Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Researchers create molecular Braille to identify DNA molecules

Abstract:
Researchers at UCLA and New York University have developed a method to detect sequence differences in individual DNA molecules by taking nanoscopic pictures of the molecules themselves.

Researchers create molecular Braille to identify DNA molecules

New York, NY | Posted on March 28th, 2012

The work is reported in the Journal of the Royal Society Interface.

Using the approach they call "Direct Molecular Recognition," the UCLA and NYU researchers used nanoparticles to turn the DNA molecules into a form of molecular braille that can be read in the scale of nanometers, or one billionth of a meter, using high-speed Atomic Force Microscopy (AFM).

The leaders of the study are: Jason Reed, a research professor, and Professor Jim Gimzewski, nanotechnology pioneer, both at UCLA's California Nanosystems Institute, and Professor Bud Mishra, genomics expert, at NYU's Courant Institute of Mathematical Sciences. This group believes the method will have many practical uses, such as super-sensitive detection of DNA molecules in genomic research and medical diagnostics as well as in identifying pathogens.

While there are a variety of techniques currently used for this purpose, they are time consuming, technically difficult, and expensive. They also require a significant amount of genetic material in order to make accurate readings and often require prior knowledge of the sample composition.

According to Mishra, to overcome these shortcomings, the team devised a "single-cell, single-molecule" method that would dispense with the complex chemical manipulations on which existing methods are based, and, instead, utilize the unique shapes of the molecules themselves as the method of identification. This approach has the benefits of being rapid and sensitive to the level of a single molecule.

Reed says that "the long term goal of our team's research is to dissect, understand, and control the biology of single cells in complex tissues, such as brain, or in malignant tumors. Furthering this body of work requires that we address an unsolved problem in single-cell molecular analysis: the lack of a method to routinely, reliably, and inexpensively determine global gene transcriptional activity."

In their paper, the team closely examined the potential use of this technique to quantify the activity of genes in living tissue, a method known as transcriptional profiling. They were able to show that their Direct Molecular Recognition technique could accurately quantitate the relative abundance of multiple DNA species in a mixture using only a handful of molecules - a result not achievable using other methods.

Their study was supported by a grant to from the National Institute of General Medical Sciences, part of the National Institutes of Health.

####

For more information, please click here

Contacts:
James Devitt

212-998-6808

Copyright © New York University

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Scientific breakthrough in rechargeable batteries: Researchers from Singapore and Québec Team Up to Develop Next-Generation Materials to Power Electronic Devices and Electric Vehicles February 28th, 2015

First detailed microscopy evidence of bacteria at the lower size limit of life: Berkeley Lab research provides comprehensive description of ultra-small bacteria February 28th, 2015

Leti to Offer Updates on Silicon Photonics Successes at OFC in LA February 27th, 2015

Moving molecule writes letters: Caging of molecules allows investigation of equilibrium thermodynamics February 27th, 2015

Imaging

Renishaw and Bruker team up for a workshop on TERS and co-localised AFM Raman February 26th, 2015

Real-time observation of bond formation by using femtosecond X-ray liquidography February 26th, 2015

Bruker-Sponsored Sixth AFM BioMed Conference Highlights Increasing Impact of AFM in Biological Applications February 26th, 2015

Dendrite eraser: New electrolyte rids batteries of short-circuiting fibers: Solution enables a battery with both high efficiency & current density February 24th, 2015

Govt.-Legislation/Regulation/Funding/Policy

First detailed microscopy evidence of bacteria at the lower size limit of life: Berkeley Lab research provides comprehensive description of ultra-small bacteria February 28th, 2015

Warming up the world of superconductors: Clusters of aluminum metal atoms become superconductive at surprisingly high temperatures February 25th, 2015

SUNY Poly CNSE Researchers and Corporate Partners to Present Forty Papers at Globally Recognized Lithography Conference: SUNY Poly CNSE Research Group Awarded Both ‘Best Research Paper’ and ‘Best Research Poster’ at SPIE Advanced Lithography 2015 forum February 25th, 2015

European roadmap for graphene science and technology published February 25th, 2015

Nanomedicine

Untangling DNA with a droplet of water, a pipet and a polymer: With the 'rolling droplet technique,' a DNA-injected water droplet rolls like a ball over a platelet, sticking the DNA to the plate surface February 27th, 2015

Graphene shows potential as novel anti-cancer therapeutic strategy: University of Manchester scientists have used graphene to target and neutralise cancer stem cells while not harming other cells February 26th, 2015

Cutting-edge technology optimizes cancer therapy with nanomedicine drug combinations: UCLA bioengineers develop platform that offers personalized approach to treatment February 24th, 2015

Optical nanoantennas set the stage for a NEMS lab-on-a-chip revolution February 24th, 2015

Discoveries

First detailed microscopy evidence of bacteria at the lower size limit of life: Berkeley Lab research provides comprehensive description of ultra-small bacteria February 28th, 2015

Leti to Offer Updates on Silicon Photonics Successes at OFC in LA February 27th, 2015

Moving molecule writes letters: Caging of molecules allows investigation of equilibrium thermodynamics February 27th, 2015

Untangling DNA with a droplet of water, a pipet and a polymer: With the 'rolling droplet technique,' a DNA-injected water droplet rolls like a ball over a platelet, sticking the DNA to the plate surface February 27th, 2015

Announcements

Scientific breakthrough in rechargeable batteries: Researchers from Singapore and Québec Team Up to Develop Next-Generation Materials to Power Electronic Devices and Electric Vehicles February 28th, 2015

First detailed microscopy evidence of bacteria at the lower size limit of life: Berkeley Lab research provides comprehensive description of ultra-small bacteria February 28th, 2015

Leti to Offer Updates on Silicon Photonics Successes at OFC in LA February 27th, 2015

Moving molecule writes letters: Caging of molecules allows investigation of equilibrium thermodynamics February 27th, 2015

Tools

Hiden CATLAB Microreactor System at ARABLAB 2015 | Visit us on Booth 1011 February 26th, 2015

Renishaw and Bruker team up for a workshop on TERS and co-localised AFM Raman February 26th, 2015

Maximum Precision in 3D Printing: New complete solution makes additive manufacturing standard for microfabrication February 26th, 2015

Real-time observation of bond formation by using femtosecond X-ray liquidography February 26th, 2015

Nanobiotechnology

Untangling DNA with a droplet of water, a pipet and a polymer: With the 'rolling droplet technique,' a DNA-injected water droplet rolls like a ball over a platelet, sticking the DNA to the plate surface February 27th, 2015

Bacteria network for food: Bacteria connect to each other and exchange nutrients February 23rd, 2015

Building tailor-made DNA nanotubes step by step: New, block-by-block assembly method could pave way for applications in opto-electronics, drug delivery February 23rd, 2015

Better batteries inspired by lowly snail shells: Biological molecules can latch onto nanoscale components and lock them into position to make high performing Li-ion battery electrodes, according to new research presented at the 59th annual meeting of the Biophysical Society February 12th, 2015

Research partnerships

Scientific breakthrough in rechargeable batteries: Researchers from Singapore and Québec Team Up to Develop Next-Generation Materials to Power Electronic Devices and Electric Vehicles February 28th, 2015

Moving molecule writes letters: Caging of molecules allows investigation of equilibrium thermodynamics February 27th, 2015

European roadmap for graphene science and technology published February 25th, 2015

KIT Increases Commitment in Asia: DAAD Funds Two New Projects: Strategic Partnerships with Chinese Universities and Communi-cation Technologies Network February 22nd, 2015

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2015 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE