Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Fluorescent Clusters Provide Window into Live Cells

Abstract:
Important information about live cells' behavior and environment may be gained using a new, easy to make fluorescent gold nanocluster, say scientists in Germany and USA.

Fluorescent Clusters Provide Window into Live Cells

Germany | Posted on August 29th, 2011

Studying the behavior of live cells is vastly important to help us understand how our own cells react to changes in environment or invasion, and also to devise better ways to control and contain hostile microbes. To visualize the cells and their processes, labels of some sort are usually required; many of these are fluorescent and can be tracked using external microscopy. Such labels can be laborious to produce and their stability and biocompatibility are a primary concern. Recent advances in nanotechnology have given rise to a new class of fluorescent labels, fluorescent metal nanoclusters. These tiny clusters of metal are small and highly fluorescent and have particular electronic properties because of their size. Their stability can be severely affected by the choice of ligand that sits on the outside of the cluster.

Now, researchers at Karlsruhe Institute of Technology, Germany and University of Illinois in the USA, led by Professor Ulrich Nienhaus, have developed an easy way to make water-soluble fluorescent gold nanoclusters (Au NCs) stabilized with a ligand called dihydrolipoic acid (DHLA). DHLA has two connection points (bidentate) to the cluster which helps to make the cluster extra stable. These Au NCs are highly stable, have bright near-infrared fluorescence emission, are very small, and very biocompatible, all of which makes them promising as novel fluorescent probes for application in biological research. The fluorescence lifetime of the clusters is approximately twice that of background cell fluorescence, so they could easily be visualized within or around a cell using fluorescence lifetime imaging (FLIM).

But the researchers did not stop there. Keen to show just how promising their ligand-stabilized clusters are, they visualized the internalization of the Au NCs by live HeLa cells using FLIM. This technique not only reveals the uptake of the Au NCs by the cells but also provides information about the changed local environment of the Au NCs on uptake through the cell membrane. Au NCs near the cell membrane have a longer fluorescence lifetime than those actually internalized by the cells.

The scientists anticipate that their fluorescent Au NCs will find wide application in biomedical research, including intracellular drug delivery, ultrasensitive molecular diagnostics, and image-guided therapy.

####

For more information, please click here

Copyright © Wiley-VCH Materials Science Journals

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

L. Shang et al., Small ; DOI: 10.1002/smll.201100746

Related News Press

News and information

First Observation of Electronic Structure in Ag-Rh Alloy Nanoparticles Having Hydrogen Absorbing: Storage Property –Attempting to solve the mystery of why Ag-Rh alloy nanoparticles have a similar property to Pd– October 30th, 2014

Iranians Present Model to Predict Photocatalytic Process in Removal of Pollutants October 30th, 2014

Production of Biocompatible Polymers in Iran October 30th, 2014

Amorphous Coordination Polymer Particles as alternative to classical nanoplatforms for nanomedicine October 30th, 2014

Imaging

A new cheap and efficient method to improve SERS, an ultra-sensitive chemical detection technique October 28th, 2014

Molecular beacons shine light on how cells 'crawl' October 27th, 2014

National Synchrotron Light Source II Achieves 'First Light' October 23rd, 2014

NIST offers electronics industry 2 ways to snoop on self-organizing molecules October 22nd, 2014

Nanomedicine

Production of Biocompatible Polymers in Iran October 30th, 2014

Amorphous Coordination Polymer Particles as alternative to classical nanoplatforms for nanomedicine October 30th, 2014

'Electronic skin' could improve early breast cancer detection October 29th, 2014

Tiny carbon nanotube pores make big impact October 29th, 2014

Discoveries

First Observation of Electronic Structure in Ag-Rh Alloy Nanoparticles Having Hydrogen Absorbing: Storage Property –Attempting to solve the mystery of why Ag-Rh alloy nanoparticles have a similar property to Pd– October 30th, 2014

Iranians Present Model to Predict Photocatalytic Process in Removal of Pollutants October 30th, 2014

Production of Biocompatible Polymers in Iran October 30th, 2014

Amorphous Coordination Polymer Particles as alternative to classical nanoplatforms for nanomedicine October 30th, 2014

Announcements

First Observation of Electronic Structure in Ag-Rh Alloy Nanoparticles Having Hydrogen Absorbing: Storage Property –Attempting to solve the mystery of why Ag-Rh alloy nanoparticles have a similar property to Pd– October 30th, 2014

Iranians Present Model to Predict Photocatalytic Process in Removal of Pollutants October 30th, 2014

Production of Biocompatible Polymers in Iran October 30th, 2014

Amorphous Coordination Polymer Particles as alternative to classical nanoplatforms for nanomedicine October 30th, 2014

Research partnerships

First Observation of Electronic Structure in Ag-Rh Alloy Nanoparticles Having Hydrogen Absorbing: Storage Property –Attempting to solve the mystery of why Ag-Rh alloy nanoparticles have a similar property to Pd– October 30th, 2014

Sussex physicists find simple solution for quantum technology challenge October 28th, 2014

Molecular beacons shine light on how cells 'crawl' October 27th, 2014

New evidence for an exotic, predicted superconducting state October 27th, 2014

NanoNews-Digest
The latest news from around the world, FREE





  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE