Nanotechnology Now

Our NanoNews Digest Sponsors



Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Fluorescent Clusters Provide Window into Live Cells

Abstract:
Important information about live cells' behavior and environment may be gained using a new, easy to make fluorescent gold nanocluster, say scientists in Germany and USA.

Fluorescent Clusters Provide Window into Live Cells

Germany | Posted on August 29th, 2011

Studying the behavior of live cells is vastly important to help us understand how our own cells react to changes in environment or invasion, and also to devise better ways to control and contain hostile microbes. To visualize the cells and their processes, labels of some sort are usually required; many of these are fluorescent and can be tracked using external microscopy. Such labels can be laborious to produce and their stability and biocompatibility are a primary concern. Recent advances in nanotechnology have given rise to a new class of fluorescent labels, fluorescent metal nanoclusters. These tiny clusters of metal are small and highly fluorescent and have particular electronic properties because of their size. Their stability can be severely affected by the choice of ligand that sits on the outside of the cluster.

Now, researchers at Karlsruhe Institute of Technology, Germany and University of Illinois in the USA, led by Professor Ulrich Nienhaus, have developed an easy way to make water-soluble fluorescent gold nanoclusters (Au NCs) stabilized with a ligand called dihydrolipoic acid (DHLA). DHLA has two connection points (bidentate) to the cluster which helps to make the cluster extra stable. These Au NCs are highly stable, have bright near-infrared fluorescence emission, are very small, and very biocompatible, all of which makes them promising as novel fluorescent probes for application in biological research. The fluorescence lifetime of the clusters is approximately twice that of background cell fluorescence, so they could easily be visualized within or around a cell using fluorescence lifetime imaging (FLIM).

But the researchers did not stop there. Keen to show just how promising their ligand-stabilized clusters are, they visualized the internalization of the Au NCs by live HeLa cells using FLIM. This technique not only reveals the uptake of the Au NCs by the cells but also provides information about the changed local environment of the Au NCs on uptake through the cell membrane. Au NCs near the cell membrane have a longer fluorescence lifetime than those actually internalized by the cells.

The scientists anticipate that their fluorescent Au NCs will find wide application in biomedical research, including intracellular drug delivery, ultrasensitive molecular diagnostics, and image-guided therapy.

####

For more information, please click here

Copyright © Wiley-VCH Materials Science Journals

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

L. Shang et al., Small ; DOI: 10.1002/smll.201100746

Related News Press

News and information

Arrowhead to Present at BioCentury's NewsMakers in the Biotech Industry Conference September 19th, 2014

SouthWest NanoTechnologies (SWeNT) Receives NIST Small Business Innovation Research (SBIR) Phase 1 Award to Produce Greater than 99% Semiconducting Single-Wall Carbon Nanotubes September 19th, 2014

Toward optical chips: A promising light source for optoelectronic chips can be tuned to different frequencies September 19th, 2014

New research points to graphene as a flexible, low-cost touchscreen solution September 19th, 2014

The Pocket Project will develop a low-cost and accurate point-of-care test to diagnose Tuberculosis: ICN2 holds a follow-up meeting of the Project on September 18th - 19th September 18th, 2014

Imaging

IEEE International Electron Devices Meeting To Celebrate 60th Anniversary as The Leading Technical Conference for Advanced Semiconductor Devices September 18th, 2014

FEI Opens New Technology Center in Czech Republic: FEI expands its presence in Brno with the opening of a new, larger facility September 18th, 2014

New NPZ100-403 Piezo Stage from nPoint Inc. September 17th, 2014

Nanomedicine

Arrowhead to Present at BioCentury's NewsMakers in the Biotech Industry Conference September 19th, 2014

The Pocket Project will develop a low-cost and accurate point-of-care test to diagnose Tuberculosis: ICN2 holds a follow-up meeting of the Project on September 18th - 19th September 18th, 2014

New non-invasive technique could revolutionize the imaging of metastatic cancer September 17th, 2014

Recruiting bacteria to be technology innovation partners: September 17th, 2014

Discoveries

Toward optical chips: A promising light source for optoelectronic chips can be tuned to different frequencies September 19th, 2014

New research points to graphene as a flexible, low-cost touchscreen solution September 19th, 2014

CiQUS researchers design an artificial nose to detect DNA differentiation with single nucleotide resolution September 18th, 2014

Big Results Require Big Ambitions: Three young UCSB faculty receive CAREER awards from the National Science Foundation September 18th, 2014

Announcements

Arrowhead to Present at BioCentury's NewsMakers in the Biotech Industry Conference September 19th, 2014

SouthWest NanoTechnologies (SWeNT) Receives NIST Small Business Innovation Research (SBIR) Phase 1 Award to Produce Greater than 99% Semiconducting Single-Wall Carbon Nanotubes September 19th, 2014

Toward optical chips: A promising light source for optoelectronic chips can be tuned to different frequencies September 19th, 2014

New research points to graphene as a flexible, low-cost touchscreen solution September 19th, 2014

Research partnerships

Biosensors Get a Boost from Graphene Partnership: $5 Million Investment Supports Dozens of Jobs and Development of 300mm Fabrication Process and Wafer Transfer Facility September 18th, 2014

The Pocket Project will develop a low-cost and accurate point-of-care test to diagnose Tuberculosis: ICN2 holds a follow-up meeting of the Project on September 18th - 19th September 18th, 2014

Recruiting bacteria to be technology innovation partners: September 17th, 2014

Carbon Sciences Developing Breakthrough Technology to Mass-Produce Graphene -- the New Miracle Material: Company Enters Into an Agreement With the University of California, Santa Barbara (UCSB) to Fund the Further Development of a New Graphene Process September 16th, 2014

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE