Nanotechnology Now

Our NanoNews Digest Sponsors



Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Fluorescent Clusters Provide Window into Live Cells

Abstract:
Important information about live cells' behavior and environment may be gained using a new, easy to make fluorescent gold nanocluster, say scientists in Germany and USA.

Fluorescent Clusters Provide Window into Live Cells

Germany | Posted on August 29th, 2011

Studying the behavior of live cells is vastly important to help us understand how our own cells react to changes in environment or invasion, and also to devise better ways to control and contain hostile microbes. To visualize the cells and their processes, labels of some sort are usually required; many of these are fluorescent and can be tracked using external microscopy. Such labels can be laborious to produce and their stability and biocompatibility are a primary concern. Recent advances in nanotechnology have given rise to a new class of fluorescent labels, fluorescent metal nanoclusters. These tiny clusters of metal are small and highly fluorescent and have particular electronic properties because of their size. Their stability can be severely affected by the choice of ligand that sits on the outside of the cluster.

Now, researchers at Karlsruhe Institute of Technology, Germany and University of Illinois in the USA, led by Professor Ulrich Nienhaus, have developed an easy way to make water-soluble fluorescent gold nanoclusters (Au NCs) stabilized with a ligand called dihydrolipoic acid (DHLA). DHLA has two connection points (bidentate) to the cluster which helps to make the cluster extra stable. These Au NCs are highly stable, have bright near-infrared fluorescence emission, are very small, and very biocompatible, all of which makes them promising as novel fluorescent probes for application in biological research. The fluorescence lifetime of the clusters is approximately twice that of background cell fluorescence, so they could easily be visualized within or around a cell using fluorescence lifetime imaging (FLIM).

But the researchers did not stop there. Keen to show just how promising their ligand-stabilized clusters are, they visualized the internalization of the Au NCs by live HeLa cells using FLIM. This technique not only reveals the uptake of the Au NCs by the cells but also provides information about the changed local environment of the Au NCs on uptake through the cell membrane. Au NCs near the cell membrane have a longer fluorescence lifetime than those actually internalized by the cells.

The scientists anticipate that their fluorescent Au NCs will find wide application in biomedical research, including intracellular drug delivery, ultrasensitive molecular diagnostics, and image-guided therapy.

####

For more information, please click here

Copyright © Wiley-VCH Materials Science Journals

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

L. Shang et al., Small ; DOI: 10.1002/smll.201100746

Related News Press

News and information

PetLife Comments on CNN Story on Scorpion Venom Health Benefits August 27th, 2014

Nanodiamonds Are Forever: A UCSB professor’s research examines 13,000-year-old nanodiamonds from multiple locations across three continents August 27th, 2014

Aspen Aerogels, Inc. to Present at Barclays CEO Energy-Power Conference August 27th, 2014

Nanotech Security Corp. to Acquire Fortress Optical Features Ltd., a Leading Producer of Banknote Security Features August 27th, 2014

Imaging

JPK expands availability of instrumentation in the USA – appointing new distributors – launched a new web site to support the US market - AFM now available to US users August 26th, 2014

Scientists craft atomically seamless, thinnest-possible semiconductor junctions August 26th, 2014

X-ray Laser Probes Tiny Quantum Tornadoes in Superfluid Droplets: SLAC Experiment Reveals Mysterious Order in Liquid Helium August 25th, 2014

Biomimetic photodetector 'sees' in color: Rice lab uses CMOS-compatible aluminum for on-chip color detection August 25th, 2014

Nanomedicine

PetLife Comments on CNN Story on Scorpion Venom Health Benefits August 27th, 2014

The thunder god vine, assisted by nanotechnology, could shake up future cancer treatment: Targeted therapy for hepatocellular carcinoma using nanotechnology August 27th, 2014

Introducing the multi-tasking nanoparticle: Versatile particles offer a wide variety of diagnostic and therapeutic applications August 26th, 2014

Symphony of nanoplasmonic and optical resonators leads to magnificent laser-like light emission August 26th, 2014

Discoveries

The thunder god vine, assisted by nanotechnology, could shake up future cancer treatment: Targeted therapy for hepatocellular carcinoma using nanotechnology August 27th, 2014

Creation of a Highly Efficient Technique to Develop Low-Friction Materials Which Are Drawing Attention in Association with Energy Issues August 26th, 2014

Competition for Graphene: Berkeley Lab Researchers Demonstrate Ultrafast Charge Transfer in New Family of 2D Semiconductors August 26th, 2014

Symphony of nanoplasmonic and optical resonators leads to magnificent laser-like light emission August 26th, 2014

Announcements

Nanodiamonds Are Forever: A UCSB professor’s research examines 13,000-year-old nanodiamonds from multiple locations across three continents August 27th, 2014

Aspen Aerogels, Inc. to Present at Barclays CEO Energy-Power Conference August 27th, 2014

Nanotech Security Corp. to Acquire Fortress Optical Features Ltd., a Leading Producer of Banknote Security Features August 27th, 2014

Malvern specialists to deliver inaugural short course on polymer characterization at Interplas 2014 August 27th, 2014

Research partnerships

The thunder god vine, assisted by nanotechnology, could shake up future cancer treatment: Targeted therapy for hepatocellular carcinoma using nanotechnology August 27th, 2014

Scientists craft atomically seamless, thinnest-possible semiconductor junctions August 26th, 2014

Competition for Graphene: Berkeley Lab Researchers Demonstrate Ultrafast Charge Transfer in New Family of 2D Semiconductors August 26th, 2014

Symphony of nanoplasmonic and optical resonators leads to magnificent laser-like light emission August 26th, 2014

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE