Nanotechnology Now

Our NanoNews Digest Sponsors
Heifer International



Home > Press > Electronic detection of DNA nanoballs enables simple pathogen detection Peer-Reviewed Publication

Vicente Pelechano at Karolinska Institutet

CREDIT
Ryan Hull
Vicente Pelechano at Karolinska Institutet CREDIT Ryan Hull

Abstract:
Researchers at Karolinska Institute have developed a novel method using DNA Nanoballs to detect pathogens, aiming to simplify nucleic acid testing and revolutionize pathogen detection. The study's results, published in Science Advances, could pave the way for a straightforward electronic-based test capable of identifying various nucleic acids in diverse scenarios quickly and cheaply.

Electronic detection of DNA nanoballs enables simple pathogen detection Peer-Reviewed Publication

Solna, Sweden | Posted on September 8th, 2023

Principal investigator Vicent Pelechano, an associate professor at Karolinska Institute’s Department of Microbiology, Tumor and Cell Biology, is cautiously optimistic about the technology's potential to detect an array of pathogenic agents in real world settings.

“The methodology involves combining Molecular Biology (DNA Nanoball generation) and electronics (electric impedance-based quantification) to yield a pioneering detection tool”, says Vicent Pelechano.

The researchers modified an isothermal DNA amplification reaction termed LAMP to generate tiny 1-2μM DNA nanoballs if the pathogen was present in the sample. These nanoballs are then directed through minute channels and identified electrically as they traverse between two electrodes. The method has demonstrated remarkable sensitivity detecting as few as 10 target molecules and rapid results under one hour, using a compact, motionless system.

“Fast and accurate detection of genetic material is key for diagnosis, especially so in response to the emergence of novel pathogens”, says Vicent Pelechano.

During the recent COVID-19 pandemic the researchers saw an extensive use of protein-based diagnostics for rapid testing. However, these methods require time-consuming development of high-quality antibodies. In contrast, nucleic acid-based approaches offer greater ease of development, enhanced sensitivity, and inherent flexibility, according to the researchers. This new method, offering label-free detection could hasten the rollout of new diagnostic kits. Integrating affordable mass-produced electronics with lyophilized reagents, the technology possesses the potential to provide a cheap, widely deployed and scalable point-of-care device.

The team commenced this work as an extension of their previous endeavors in LAMP (Loop-Mediated Isothermal Amplification) based detection of SARS-CoV-2 during the pandemic.

Presently, the research team is actively exploring avenues to integrate this technology into domains such as environmental monitoring, food safety, virus and antimicrobial resistance detection. The team is also exploring avenues for licensing or potentially establishing a startup to capitalize on this technology, having recently applied for a patent for the technology

The main financiers of the study are the Knut and Alice Wallenberg Foundation.
Important collaboration partners are Rutgers University and the Stanford Genome Technology Center.

####

For more information, please click here

Contacts:
Press Office
Karolinska Institutet

Office: +46 8 524 86077

Copyright © Karolinska Institutet

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

ARTICLE TITLE

Related News Press

News and information

First human trial shows ‘wonder’ material can be developed safely: A revolutionary nanomaterial with huge potential to tackle multiple global challenges could be developed further without acute risk to human health, research suggests February 16th, 2024

Detecting breast cancer through a spit test February 16th, 2024

New chip opens door to AI computing at light speed February 16th, 2024

HKUST researchers develop new integration technique for efficient coupling of III-V and silicon February 16th, 2024

Possible Futures

First human trial shows ‘wonder’ material can be developed safely: A revolutionary nanomaterial with huge potential to tackle multiple global challenges could be developed further without acute risk to human health, research suggests February 16th, 2024

Detecting breast cancer through a spit test February 16th, 2024

A battery’s hopping ions remember where they’ve been: Seen in atomic detail, the seemingly smooth flow of ions through a battery’s electrolyte is surprisingly complicated February 16th, 2024

NRL discovers two-dimensional waveguides February 16th, 2024

Nanomedicine

Detecting breast cancer through a spit test February 16th, 2024

Superbug killer: New synthetic molecule highly effective against drug-resistant bacteria February 16th, 2024

Researchers develop technique to synthesize water-soluble alloy nanoclusters January 12th, 2024

Focused ion beam technology: A single tool for a wide range of applications January 12th, 2024

Discoveries

HKUST researchers develop new integration technique for efficient coupling of III-V and silicon February 16th, 2024

Electrons screen against conductivity-killer in organic semiconductors: The discovery is the first step towards creating effective organic semiconductors, which use significantly less water and energy, and produce far less waste than their inorganic counterparts February 16th, 2024

Superbug killer: New synthetic molecule highly effective against drug-resistant bacteria February 16th, 2024

Discovery of new Li ion conductor unlocks new direction for sustainable batteries: University of Liverpool researchers have discovered a new solid material that rapidly conducts lithium ions February 16th, 2024

Announcements

Detecting breast cancer through a spit test February 16th, 2024

New chip opens door to AI computing at light speed February 16th, 2024

HKUST researchers develop new integration technique for efficient coupling of III-V and silicon February 16th, 2024

Electrons screen against conductivity-killer in organic semiconductors: The discovery is the first step towards creating effective organic semiconductors, which use significantly less water and energy, and produce far less waste than their inorganic counterparts February 16th, 2024

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers/Posters

First human trial shows ‘wonder’ material can be developed safely: A revolutionary nanomaterial with huge potential to tackle multiple global challenges could be developed further without acute risk to human health, research suggests February 16th, 2024

Detecting breast cancer through a spit test February 16th, 2024

New chip opens door to AI computing at light speed February 16th, 2024

HKUST researchers develop new integration technique for efficient coupling of III-V and silicon February 16th, 2024

Nanobiotechnology

Detecting breast cancer through a spit test February 16th, 2024

Superbug killer: New synthetic molecule highly effective against drug-resistant bacteria February 16th, 2024

Researchers develop technique to synthesize water-soluble alloy nanoclusters January 12th, 2024

Focused ion beam technology: A single tool for a wide range of applications January 12th, 2024

Research partnerships

'Sudden death' of quantum fluctuations defies current theories of superconductivity: Study challenges the conventional wisdom of superconducting quantum transitions January 12th, 2024

Development of zinc oxide nanopagoda array photoelectrode: photoelectrochemical water-splitting hydrogen production January 12th, 2024

2D material reshapes 3D electronics for AI hardware December 8th, 2023

Presenting: Ultrasound-based printing of 3D materials—potentially inside the body December 8th, 2023

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project