Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > New technology can detect hundreds of proteins in a single sample: Improvement of barcoding technique offers cost-effective alternative to current technology

Abstract:
New technology developed by a team of McGill University scientists shows potential to streamline the analysis of proteins, offering a quick, high volume and cost-effective tool to hospitals and research labs alike.

New technology can detect hundreds of proteins in a single sample: Improvement of barcoding technique offers cost-effective alternative to current technology

Montreal, Canada | Posted on August 13th, 2018

Proteins found in blood provide scientists and clinicians with key information on our health. These biological markers can determine if a chest pain is caused by a cardiac event or if a patient has cancer.

Unfortunately, the tools used to detect such proteins haven’t evolved much over the past 50 years – despite there being over 20,000 proteins in our body, the vast majority of protein tests run today target only a single protein at-a-time.

Now, PhD candidate Milad Dagher, Professor David Juncker and colleagues in McGill’s Department of Biomedical Engineering have devised a technique that can detect hundreds of proteins with a single blood sample.

Part of their work, just published in Nature Nanotechnology, describes a new and improved way to barcode micro-beads using multicolour fluorescent dyes. By generating upwards of 500 differently coloured micro-beads, their new barcoding platform enables detection of markers in parallel from the same solution—for example, a blue barcode can be used to detect marker 1, while a red barcode can detect marker 2, and so on. A laser-based instrument called a cytometer then counts the proteins that stick to the different coloured beads.

Though this kind of analysis method has been available for some time, interference among multicolour dyes has limited the ability to generate the right colours. Now, a new algorithm developed by the team enables different colours of micro-beads to be generated with high accuracy—much like a colour wheel can be used to predict the outcome of colour mixing.

Professor Juncker’s team is hoping to leverage its platform for improved analysis of proteins.

“Current technologies hold a major trade-off between the number of proteins that can be measured at once, and the cost and accuracy of a test”, Dagher explains. “This means that large-scale studies, such as clinical trials, are underpowered because they tend to fall back on tried-and-true platforms with limited capabilities.”

Their upcoming work focuses on maintaining accurate detection of proteins with increased scale.

Dagher and Jeffrey Munzar, a postdoctoral fellow in the Juncker lab, have teamed up with Professor Juncker and spun-off a company, nplex biosciences, to commercialize their new approach.

The McGill group was also recently awarded an NSERC Idea to Innovation grant to support the development of the next version of their technology platform.

The authors thank NSERC and FQRNT for funding. M.D. acknowledges the NSERC-CREATE ISS programme for support. The flow cytometry work was performed at two McGill core flow facilities, namely the Microbiology and Immunology (MIMM) department and the Life Science Complex, which is supported by funding from the Canadian Foundation for Innovation.

####

For more information, please click here

Contacts:
Justin Dupuis

Media Relations

McGill University

Tel.: 514 398-6751





http://www.mcgill.ca/newsroom/

http://www.twitter.com/mcgillu

Copyright © McGill University

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

“Ensemble multicolour FRET model enables barcoding at extreme FRET levels” by Milad Dagher, Michael Kleinman, Andy Ng and David Juncker was published in Nature Nanotechnology

Related News Press

News and information

Scientists produce 3D chemical maps of single bacteria: Researchers at NSLS-II used ultrabright x-rays to generate 3-D nanoscale maps of a single bacteria's chemical composition with unparalleled spatial resolution November 16th, 2018

Bosch provides customized IoT and Industry 4.0 solutions: Bosch Mondeville and Bosch Connected Devices and Solutions collaborate to meet a wide variety of customer requirements November 16th, 2018

GaN Rising: UC Santa Barbara electrical and computer engineering professor Umesh Mishra to deliver 63rd Annual Faculty Research Lecture November 16th, 2018

Nanometrics Completes Acquisition of 4D Technology Corporation: The addition of Dynamic Interferometry® expands process control technology solutions November 16th, 2018

Imaging

Scientists produce 3D chemical maps of single bacteria: Researchers at NSLS-II used ultrabright x-rays to generate 3-D nanoscale maps of a single bacteria's chemical composition with unparalleled spatial resolution November 16th, 2018

Tiny light detectors work like gecko ears October 30th, 2018

Cancer

WSU researchers develop new technique to understand biology at the nanoscale November 7th, 2018

New platform based on biology and nanotechnology carries mRNA directly to target cells: Combined platform provides safe, effective passage for therapies treating cancer and other diseases, Tel Aviv University researchers say October 29th, 2018

Shape-shifting sensors could catch early signs of cancer October 19th, 2018

Govt.-Legislation/Regulation/Funding/Policy

Scientists produce 3D chemical maps of single bacteria: Researchers at NSLS-II used ultrabright x-rays to generate 3-D nanoscale maps of a single bacteria's chemical composition with unparalleled spatial resolution November 16th, 2018

'Smart skin' simplifies spotting strain in structures: Rice U. invention can use fluorescing carbon nanotubes to reveal stress in aircraft, structures November 15th, 2018

Epoxy compound gets a graphene bump: Rice scientists combine graphene foam, epoxy into tough, conductive composite November 14th, 2018

European Commission Project Creates Pilot Line for Companies to Develop Mid-Infrared Devices: Companies Can Submit Proposals for Possible Matching Funds To Help Develop Prototypes November 13th, 2018

Possible Futures

Scientists produce 3D chemical maps of single bacteria: Researchers at NSLS-II used ultrabright x-rays to generate 3-D nanoscale maps of a single bacteria's chemical composition with unparalleled spatial resolution November 16th, 2018

GaN Rising: UC Santa Barbara electrical and computer engineering professor Umesh Mishra to deliver 63rd Annual Faculty Research Lecture November 16th, 2018

'Smart skin' simplifies spotting strain in structures: Rice U. invention can use fluorescing carbon nanotubes to reveal stress in aircraft, structures November 15th, 2018

Epoxy compound gets a graphene bump: Rice scientists combine graphene foam, epoxy into tough, conductive composite November 14th, 2018

Nanomedicine

Scientists produce 3D chemical maps of single bacteria: Researchers at NSLS-II used ultrabright x-rays to generate 3-D nanoscale maps of a single bacteria's chemical composition with unparalleled spatial resolution November 16th, 2018

Arrowhead Pharmaceuticals Presents Late-Breaking Clinical Data on ARO-AAT at Liver Meeting® 2018 November 9th, 2018

Arrowhead Pharmaceuticals Presents Late-Breaking Preliminary Clinical Data on ARO-HBV at Liver Meeting® 2018 November 9th, 2018

WSU researchers develop new technique to understand biology at the nanoscale November 7th, 2018

Discoveries

Scientists produce 3D chemical maps of single bacteria: Researchers at NSLS-II used ultrabright x-rays to generate 3-D nanoscale maps of a single bacteria's chemical composition with unparalleled spatial resolution November 16th, 2018

'Smart skin' simplifies spotting strain in structures: Rice U. invention can use fluorescing carbon nanotubes to reveal stress in aircraft, structures November 15th, 2018

Epoxy compound gets a graphene bump: Rice scientists combine graphene foam, epoxy into tough, conductive composite November 14th, 2018

Optimization of alloy materials: Diffusion processes in nano particles decoded November 13th, 2018

Announcements

Scientists produce 3D chemical maps of single bacteria: Researchers at NSLS-II used ultrabright x-rays to generate 3-D nanoscale maps of a single bacteria's chemical composition with unparalleled spatial resolution November 16th, 2018

Bosch provides customized IoT and Industry 4.0 solutions: Bosch Mondeville and Bosch Connected Devices and Solutions collaborate to meet a wide variety of customer requirements November 16th, 2018

GaN Rising: UC Santa Barbara electrical and computer engineering professor Umesh Mishra to deliver 63rd Annual Faculty Research Lecture November 16th, 2018

Nanometrics Completes Acquisition of 4D Technology Corporation: The addition of Dynamic Interferometry® expands process control technology solutions November 16th, 2018

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

Scientists produce 3D chemical maps of single bacteria: Researchers at NSLS-II used ultrabright x-rays to generate 3-D nanoscale maps of a single bacteria's chemical composition with unparalleled spatial resolution November 16th, 2018

'Smart skin' simplifies spotting strain in structures: Rice U. invention can use fluorescing carbon nanotubes to reveal stress in aircraft, structures November 15th, 2018

Epoxy compound gets a graphene bump: Rice scientists combine graphene foam, epoxy into tough, conductive composite November 14th, 2018

Optimization of alloy materials: Diffusion processes in nano particles decoded November 13th, 2018

Nanobiotechnology

Scientists produce 3D chemical maps of single bacteria: Researchers at NSLS-II used ultrabright x-rays to generate 3-D nanoscale maps of a single bacteria's chemical composition with unparalleled spatial resolution November 16th, 2018

Arrowhead Pharmaceuticals Presents Late-Breaking Clinical Data on ARO-AAT at Liver Meeting® 2018 November 9th, 2018

Arrowhead Pharmaceuticals Presents Late-Breaking Preliminary Clinical Data on ARO-HBV at Liver Meeting® 2018 November 9th, 2018

WSU researchers develop new technique to understand biology at the nanoscale November 7th, 2018

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project