Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Biologists Develop Nanosensors to Visualize Movements and Distribution of Plant Stress Hormone

Time sequence shows how mustard seedlings take up and distribute ABA through roots and other parts of the plant during germination. Credit: Rainer Waadt, UC San Diego
Time sequence shows how mustard seedlings take up and distribute ABA through roots and other parts of the plant during germination.

Credit: Rainer Waadt, UC San Diego

Abstract:
Biologists at UC San Diego have succeeded in visualizing the movement within plants of a key hormone responsible for growth and resistance to drought. The achievement will allow researchers to conduct further studies to determine how the hormone helps plants respond to drought and other environmental stresses driven by the continuing increase in the atmosphere's carbon dioxide, or CO2, concentration.

Biologists Develop Nanosensors to Visualize Movements and Distribution of Plant Stress Hormone

San Diego, CA | Posted on April 15th, 2014

The plant hormone the biologists directly tracked is abscisic acid, or ABA, which plays a major role in activating drought resistance responses of plants and in regulating plant growth under environmental stress conditions. The ABA stress hormone also controls the closing of stomata, the pores within leaves through which plants lose 95 percent of their water while taking in CO2 for growth.

Scientists already know the general role that ABA plays within plants, but by directly visualizing the hormone they can now better understand the complex interactions involving ABA when a plant is subjected to drought or other stress.

"Understanding the dynamic distribution of ABA in plants in response to environmental stimuli is of particular importance in elucidating the action of this important plant hormone," says Julian Schroeder, a professor of biology at UC San Diego who headed the research effort. "For example, we can now investigate whether an increase in the leaf CO2 concentration that occurs every night due to respiration in leaves affects the ABA concentration in stomatal cells."

The researchers developed what they call a "genetically-encoded reporter" in order to directly and instantaneously observe the movements of ABA within the mustard plant Arabidopsis. These reporters, called "ABAleons," contain two differentially colored fluorescent proteins attached to an ABA-binding sensor protein. Once bound to ABA, the ABAleons change their fluorescence emission, which can be analyzed using a microscope. The researchers showed that ABA concentration changes and waves of ABA movement could be monitored in diverse tissues and individual cells over time and in response to stress.

"Using this reporter, we directly observed long distance ABA movements from the stem of a germinating seedling to the leaves and roots of the growing plant and, for the first time, we were able to determine the rate of ABA movement within the growing plant," says Schroeder.

"Using this tool, we now can detect ABA in live plants and see how it is distributed," says Rainer Waadt, a postdoctoral associate in Schroeder's laboratory and the first author of the paper. "We are also able to directly see that environmental stress causes an increase in the ABA concentration in the stomatal guard cells that surround each stomatal pore. In the future, our sensors can be used to study ABA distribution in response to different stresses, including CO2 elevations, and to identify other molecules and proteins that affect the distribution of this hormone. We can also learn how fast plants respond to stresses and which tissues are important for the response."

The researchers demonstrated that their new ABA nanosensors also function effectively as isolated proteins. This means that the sensors could be directly employed using state-of-the-art high-throughput screening platforms to screen for chemicals that could activate or enhance a drought resistance response. The scientists say such chemicals could become useful in the future for enhancing a drought resistance response, when crops experience a severe drought, like the one that occurred in the Midwest in the summer of 2012.

The study was supported by grants from the National Science Foundation and, in part, the National Institutes of Health and the U.S Department of Energy's Division of Chemical Sciences, Geosciences, and Biosciences in the Office of Basic Energy Sciences.

####

For more information, please click here

Contacts:
Kim McDonald

858-534-7572

Copyright © University of California - San Diego

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

A paper describing their achievement appears in the April 15 issue of the scientific journal eLife and is accessible here:

Schroeder Lab:

Related News Press

News and information

'Lasers rewired': Scientists find a new way to make nanowire lasers: Berkeley Lab, UC Berkeley scientists adapt next-gen solar cell materials for a different purpose February 12th, 2016

Breaking cell barriers with retractable protein nanoneedles: Adapting a bacterial structure, Wyss Institute researchers develop protein actuators that can mechanically puncture cells February 12th, 2016

Replacement of Toxic Antibacterial Agents Possible by Biocompatible Polymeric Nanocomposites February 12th, 2016

Properties of Polymeric Nanofibers Optimized to Treat Damaged Body Tissues February 12th, 2016

Videos/Movies

The iron stepping stones to better wearable tech without semiconductors February 8th, 2016

Novel nanoparticle made of common mineral may help keep tumor growth at bay February 4th, 2016

New invention revolutionizes heat transport February 1st, 2016

Nano-coating makes coaxial cables lighter: Rice University scientists replace metal with carbon nanotubes for aerospace use January 28th, 2016

Govt.-Legislation/Regulation/Funding/Policy

Graphene leans on glass to advance electronics: Scientists' use of common glass to optimize graphene's electronic properties could improve technologies from flat screens to solar cells February 12th, 2016

A metal that behaves like water: Researchers describe new behaviors of graphene February 12th, 2016

'Lasers rewired': Scientists find a new way to make nanowire lasers: Berkeley Lab, UC Berkeley scientists adapt next-gen solar cell materials for a different purpose February 12th, 2016

Silicon chip with integrated laser: Light from a nanowire: Nanolaser for information technology February 12th, 2016

Sensors

Scientists have put a high precision blood assay into a simple test strip: Researchers have developed a new biosensor test system based on magnetic nanoparticles February 3rd, 2016

Nanosheet growth technique could revolutionize nanomaterial production February 1st, 2016

New record in nanoelectronics at ultralow temperatures January 28th, 2016

NBC LEARN DEBUTS SIX-PART VIDEO SERIES, “NANOTECHNOLOGY: SUPER SMALL SCIENCE” Produced by NBC Learn in partnership with the National Science Foundation, and narrated by NBC News/MSNBC’s Kate Snow, series highlights leading research in nanotechnology January 25th, 2016

Discoveries

'Lasers rewired': Scientists find a new way to make nanowire lasers: Berkeley Lab, UC Berkeley scientists adapt next-gen solar cell materials for a different purpose February 12th, 2016

Breaking cell barriers with retractable protein nanoneedles: Adapting a bacterial structure, Wyss Institute researchers develop protein actuators that can mechanically puncture cells February 12th, 2016

Replacement of Toxic Antibacterial Agents Possible by Biocompatible Polymeric Nanocomposites February 12th, 2016

Properties of Polymeric Nanofibers Optimized to Treat Damaged Body Tissues February 12th, 2016

Announcements

Graphene leans on glass to advance electronics: Scientists' use of common glass to optimize graphene's electronic properties could improve technologies from flat screens to solar cells February 12th, 2016

Breaking cell barriers with retractable protein nanoneedles: Adapting a bacterial structure, Wyss Institute researchers develop protein actuators that can mechanically puncture cells February 12th, 2016

Replacement of Toxic Antibacterial Agents Possible by Biocompatible Polymeric Nanocomposites February 12th, 2016

Properties of Polymeric Nanofibers Optimized to Treat Damaged Body Tissues February 12th, 2016

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

'Lasers rewired': Scientists find a new way to make nanowire lasers: Berkeley Lab, UC Berkeley scientists adapt next-gen solar cell materials for a different purpose February 12th, 2016

Breaking cell barriers with retractable protein nanoneedles: Adapting a bacterial structure, Wyss Institute researchers develop protein actuators that can mechanically puncture cells February 12th, 2016

Replacement of Toxic Antibacterial Agents Possible by Biocompatible Polymeric Nanocomposites February 12th, 2016

Properties of Polymeric Nanofibers Optimized to Treat Damaged Body Tissues February 12th, 2016

Food/Agriculture/Supplements

Replacement of Toxic Antibacterial Agents Possible by Biocompatible Polymeric Nanocomposites February 12th, 2016

Scientists have put a high precision blood assay into a simple test strip: Researchers have developed a new biosensor test system based on magnetic nanoparticles February 3rd, 2016

Herbal Extracts Applied to Synthesize Titanium Dioxide Nanoparticles January 28th, 2016

Antibacterial Nanocomposites Designed in Iran for Foodstuff Packaging January 20th, 2016

Nanobiotechnology

Breaking cell barriers with retractable protein nanoneedles: Adapting a bacterial structure, Wyss Institute researchers develop protein actuators that can mechanically puncture cells February 12th, 2016

Replacement of Toxic Antibacterial Agents Possible by Biocompatible Polymeric Nanocomposites February 12th, 2016

Properties of Polymeric Nanofibers Optimized to Treat Damaged Body Tissues February 12th, 2016

SLAC X-ray laser turns crystal imperfections into better images of important biomolecules: New method could remove major obstacles to studying structures of complex biological machines February 11th, 2016

NanoNews-Digest
The latest news from around the world, FREE





  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







Car Brands
Buy website traffic