Nanotechnology Now







Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Biologists Develop Nanosensors to Visualize Movements and Distribution of Plant Stress Hormone

Time sequence shows how mustard seedlings take up and distribute ABA through roots and other parts of the plant during germination. Credit: Rainer Waadt, UC San Diego
Time sequence shows how mustard seedlings take up and distribute ABA through roots and other parts of the plant during germination.

Credit: Rainer Waadt, UC San Diego

Abstract:
Biologists at UC San Diego have succeeded in visualizing the movement within plants of a key hormone responsible for growth and resistance to drought. The achievement will allow researchers to conduct further studies to determine how the hormone helps plants respond to drought and other environmental stresses driven by the continuing increase in the atmosphere's carbon dioxide, or CO2, concentration.

Biologists Develop Nanosensors to Visualize Movements and Distribution of Plant Stress Hormone

San Diego, CA | Posted on April 15th, 2014

The plant hormone the biologists directly tracked is abscisic acid, or ABA, which plays a major role in activating drought resistance responses of plants and in regulating plant growth under environmental stress conditions. The ABA stress hormone also controls the closing of stomata, the pores within leaves through which plants lose 95 percent of their water while taking in CO2 for growth.

Scientists already know the general role that ABA plays within plants, but by directly visualizing the hormone they can now better understand the complex interactions involving ABA when a plant is subjected to drought or other stress.

"Understanding the dynamic distribution of ABA in plants in response to environmental stimuli is of particular importance in elucidating the action of this important plant hormone," says Julian Schroeder, a professor of biology at UC San Diego who headed the research effort. "For example, we can now investigate whether an increase in the leaf CO2 concentration that occurs every night due to respiration in leaves affects the ABA concentration in stomatal cells."

The researchers developed what they call a "genetically-encoded reporter" in order to directly and instantaneously observe the movements of ABA within the mustard plant Arabidopsis. These reporters, called "ABAleons," contain two differentially colored fluorescent proteins attached to an ABA-binding sensor protein. Once bound to ABA, the ABAleons change their fluorescence emission, which can be analyzed using a microscope. The researchers showed that ABA concentration changes and waves of ABA movement could be monitored in diverse tissues and individual cells over time and in response to stress.

"Using this reporter, we directly observed long distance ABA movements from the stem of a germinating seedling to the leaves and roots of the growing plant and, for the first time, we were able to determine the rate of ABA movement within the growing plant," says Schroeder.

"Using this tool, we now can detect ABA in live plants and see how it is distributed," says Rainer Waadt, a postdoctoral associate in Schroeder's laboratory and the first author of the paper. "We are also able to directly see that environmental stress causes an increase in the ABA concentration in the stomatal guard cells that surround each stomatal pore. In the future, our sensors can be used to study ABA distribution in response to different stresses, including CO2 elevations, and to identify other molecules and proteins that affect the distribution of this hormone. We can also learn how fast plants respond to stresses and which tissues are important for the response."

The researchers demonstrated that their new ABA nanosensors also function effectively as isolated proteins. This means that the sensors could be directly employed using state-of-the-art high-throughput screening platforms to screen for chemicals that could activate or enhance a drought resistance response. The scientists say such chemicals could become useful in the future for enhancing a drought resistance response, when crops experience a severe drought, like the one that occurred in the Midwest in the summer of 2012.

The study was supported by grants from the National Science Foundation and, in part, the National Institutes of Health and the U.S Department of Energy's Division of Chemical Sciences, Geosciences, and Biosciences in the Office of Basic Energy Sciences.

####

For more information, please click here

Contacts:
Kim McDonald

858-534-7572

Copyright © University of California - San Diego

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

A paper describing their achievement appears in the April 15 issue of the scientific journal eLife and is accessible here:

Schroeder Lab:

Related News Press

News and information

Rutgers, NIST physicists report technology with potential for sub-micron optical switches March 31st, 2015

Prototype 'nanoneedles' generate new blood vessels in mice: Scientists have developed tiny 'nanoneedles' that have successfully prompted parts of the body to generate new blood vessels, in a trial in mice March 31st, 2015

Super sensitive measurement of magnetic fields March 31st, 2015

Nanomedicine pioneer Mauro Ferrari at ETH Zurich March 31st, 2015

Videos/Movies

Novel nanoparticle therapy promotes wound healing March 27th, 2015

Carbon nanotube fibers make superior links to brain: Rice University invention provides two-way communication with neurons March 25th, 2015

ASIC Development for MEMS Applications: A Platform Approach March 25th, 2015

First proof of isolated attosecond pulse generation at the carbon K-edge March 20th, 2015

Govt.-Legislation/Regulation/Funding/Policy

Rutgers, NIST physicists report technology with potential for sub-micron optical switches March 31st, 2015

SUNY Poly CNSE and Title Sponsor SEFCU Name Capital Region Teams Advancing to the Final Round of the 2015 New York Business Plan Competition March 30th, 2015

Princess Margaret scientists convert microbubbles to nanoparticles: Harnessing light to advance tumor imaging, provide platform for targeted treatment March 30th, 2015

Nanoscale worms provide new route to nano-necklace structures March 29th, 2015

Sensors

From tobacco to cyberwood March 31st, 2015

UW scientists build a nanolaser using a single atomic sheet March 24th, 2015

Iranian Researchers Present Model to Determine Dynamic Behavior of Nanostructures March 24th, 2015

Nanodevice Invented in Iran to Detect Hydrogen Sulfide in Oil, Gas Industry March 20th, 2015

Discoveries

Rutgers, NIST physicists report technology with potential for sub-micron optical switches March 31st, 2015

Prototype 'nanoneedles' generate new blood vessels in mice: Scientists have developed tiny 'nanoneedles' that have successfully prompted parts of the body to generate new blood vessels, in a trial in mice March 31st, 2015

Super sensitive measurement of magnetic fields March 31st, 2015

From tobacco to cyberwood March 31st, 2015

Announcements

Rutgers, NIST physicists report technology with potential for sub-micron optical switches March 31st, 2015

Prototype 'nanoneedles' generate new blood vessels in mice: Scientists have developed tiny 'nanoneedles' that have successfully prompted parts of the body to generate new blood vessels, in a trial in mice March 31st, 2015

Super sensitive measurement of magnetic fields March 31st, 2015

Nanomedicine pioneer Mauro Ferrari at ETH Zurich March 31st, 2015

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

Nanomedicine shines light on combined force of nanomedicine and regenerative medicine March 31st, 2015

Prototype 'nanoneedles' generate new blood vessels in mice: Scientists have developed tiny 'nanoneedles' that have successfully prompted parts of the body to generate new blood vessels, in a trial in mice March 31st, 2015

Super sensitive measurement of magnetic fields March 31st, 2015

From tobacco to cyberwood March 31st, 2015

Food/Agriculture/Supplements

Click! That's how modern chemistry bonds nanoparticles to a substrate March 19th, 2015

EU Funded PCATDES Project has completed its half-period with success March 19th, 2015

Turmeric Extract Applied in Production of Antibacterial Nanodrugs March 12th, 2015

Simple, Cost-Efficient Method Used to Determine Toxicants Growing in Pistachio February 26th, 2015

Nanobiotechnology

From tobacco to cyberwood March 31st, 2015

Wrapping carbon nanotubes in polymers enhances their performance: Scientists at Japan's Kyushu University say polymer-wrapped carbon nanotubes hold much promise in biotechnology and energy applications March 30th, 2015

'Atomic chicken-wire' is key to faster DNA sequencing March 30th, 2015

Designer's toolkit for dynamic DNA nanomachines: Arm-waving nanorobot signals new flexibility in DNA origami March 27th, 2015

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2015 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE