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Home > Press > Malvern specialist to explore use of Nanoparticle Tracking Analysis for characterizing extracellular vesicles at ISEV 2014

Abstract:
Pauline Carnell, Senior Application Scientist at Malvern Instruments, will present a paper at ISEV 2014 discussing ‘The practical use of nanoparticle tracking analysis (NTA) to characterise extracellular vesicles in general and when using fluorescence markers'. ISEV 2014 is a meeting of the International Society for Extracellular Vesicles. It takes place in Rotterdam, The Netherlands, from 30 April to 3 May 2014, with an education day on 29 April. Pauline Carnell will present the paper, co-authored with colleagues Andrew Malloy, Agnieszka Siupa, Patrick Hole and Bob Carr, on 30 April. All the authors are experts in the technology and application of NanoSight Nanoparticle Tracking Analysis (NTA). NanoSight NTA systems are now offered as part of Malvern's portfolio of analytical solutions for characterization at the nanoscale and Malvern is a sponsor for ISEV 2014: www.isevmeeting.org

Malvern specialist to explore use of Nanoparticle Tracking Analysis for characterizing extracellular vesicles at ISEV 2014

Malvern, UK | Posted on March 11th, 2014

Extracellular vesicles, typically 30 to 100 nm, are released within and from a wide variety of cells. They are increasingly implicated and appear in a multitude of pathological conditions and show much promise as diagnostics for many different diseases including cancer, heart disease, diabetes, Alzheimer's and pre-eclampsia. However, developments in this area are constrained by limitations in the technology available for their measurement.

NanoSight Nanoparticle Tracking Analysis offers the potential to rapidly enumerate and speciate these particles. In this method the particles in suspension scatter laser light, which is collected by a CCD (or sCMOS) camera via a microscope-type configuration. Particles are tracked individually and their diffusion coefficient, and therefore size, calculated directly from their speed. This gives a direct measurement of the concentration and size distribution of the particles in the field of view.

Fluorescent labelling of particles for expressed markers enables measurement of the concentration and size distribution of those labelled particles only, allowing the proportion of labelled particles to be calculated. Pauline Carnell's presentation will examine some of the challenges and considerations required during protocol development and measurement, and will include examples of good methodologies and appropriate controls.

For details of ISEV 2014 go to www.isev.org

Download the latest white paper on Nanoparticle Tracking Analysis for Extracellular Vesicles at bit.ly/MALEV

Malvern, Malvern Instruments and NanoSight are registered trademarks of Malvern Instruments Ltd

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About Malvern Instruments
Malvern provides the materials and biophysical characterization technology and expertise that enables scientists and engineers to understand and control the properties of dispersed systems. These systems range from proteins and polymers in solution, particle and nanoparticle suspensions and emulsions, through to sprays and aerosols, industrial bulk powders and high concentration slurries. Used at all stages of research, development and manufacturing, Malvern’s materials characterization instruments provide critical information that helps accelerate research and product development, enhance and maintain product quality and optimize process efficiency.

Our products reflect Malvern’s drive to exploit the latest technological innovations and our commitment to maximizing the potential of established techniques. They are used by both industry and academia, in sectors ranging from pharmaceuticals and biopharmaceuticals to bulk chemicals, cement, plastics and polymers, energy and the environment.

Malvern systems are used to measure particle size, particle shape, zeta potential, protein charge, molecular weight, mass, size and conformation, rheological properties and for chemical identification, advancing the understanding of dispersed systems across many different industries and applications.

Headquartered in Malvern, UK, Malvern Instruments has subsidiary organizations in all major European markets, North America, China, Japan and Korea, a joint venture in India, a global distributor network and applications laboratories around the world.

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Contacts:
For press information, please contact:

Trish Appleton
Kapler Communications
Phoenix House, Phoenix Park
Eaton Socon, Cambridgeshire, PE19 8EP, UK
Tel: +44 (0)1480 471059
Fax: +44 (0)1480 471069


USA contact:

Marisa Fraser
Malvern Instruments Inc.
117 Flanders Road
Westborough, MA 01581-1042 USA
Tel: +1 508 768 6400
Fax: +1 508 768 6403


Please send sales enquiries to:

Alison Vines
Malvern Instruments Ltd
Enigma Business Park, Grovewood Road
Malvern, Worcestershire WR14 1XZ UK
Tel: +44 (0) 1684 892456
Fax: +44 (0) 1684 892789

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