Nanotechnology Now

Our NanoNews Digest Sponsors



Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Malvern reports on how NanoSight's Nanoparticle Tracking Analysis, NTA, is being applied in the Veterinary Research Institute, Brno, Czech Republic

Users of the Malvern NanoSight NS500 at the VRI, Brno in the Czech Republic
Users of the Malvern NanoSight NS500 at the VRI, Brno in the Czech Republic

Abstract:
Malvern reports on how NanoSight's Nanoparticle Tracking Analysis, NTA, is being applied in the Veterinary Research Institute, Brno, Czech Republic in the research group of Dr. Jaroslav Turanek in the Department of Pharmacology and Immunotherapy).

Malvern reports on how NanoSight's Nanoparticle Tracking Analysis, NTA, is being applied in the Veterinary Research Institute, Brno, Czech Republic

Malvern, UK | Posted on January 7th, 2014

The central theme of Dr. Jaroslav Turanek's research group (Department of Pharmacology and Immunotherapy) at the Veterinary Research Institute in Brno is to apply synthetic and bioorganic chemistry. This work is performed in collaboration with King's College London and the Institute of Organic Chemistry and Biochemistry, Prague, for the design and construction of therapeutic nanoparticles to develop drug delivery systems (anticancer and antiviral drugs) and nanocarriers for construction of recombinant vaccines.

In parallel, the research group of Dr. Miroslav Machala (Department of Chemistry and Toxicology) at Veterinary Research Institute focuses upon environmental nanoparticulate pollutants. Characterization of airborne particles is conducted using electron microscopy, but in vitro tests on cell culture require knowledge of the real structure of nanoparticles in the tissue culture medium (e.g. aggregation). This enables the group to draw the correct conclusions from in vitro toxicological experiments which can be affected by differences in local nanoparticle concentration owing to sedimentation. Detailed particle distribution and kinetics of aggregation in this heterogeneous system is impossible to obtain using electron microscopy and hence Nanoparticle Tracking Analysis, NTA, is the method of choice. It is noted that some metastable aggregates can disaggregate due to high dilution of the sample required for NTA analysis. For this reason, Dynamic Light Scattering, DLS, and NTA are used as suitable complementary methods in the laboratory.

The Malvern NanoSight NS500 system is used in the VRI laboratories as alongside other techniques including DLS (Zetasizer Nano ZS), static light scattering, Gel Permeation Chromatography, electron microscopy and Field Flow Fractionation.

Explaining their choice of NanoSight, Dr Turanek said "We chose NTA as a convenient and rapid method for characterization of nanoparticles in heterogeneous preparations like liposomes and their complexes with proteins, DNA and polysaccharides. A set of these techniques is used for the complex characterization of the structure of the nanoparticles, the kinetics of their preparation, the dynamics of morphological transformation and, finally, their stability. NTA perfectly fits our needs and has become a standard method in our methodological portfolio. The most advantageous feature of NTA is that it makes it possible to visualize each nanoparticle and then to obtain more detailed size distributions based on individual particle measurements. DLS is used as precise complementary method for the characterization of nanoparticles below 20 nm for proteins and other biopolymers. Combination of these two methods, NTA and DLS, with separation methods (GPC, FFF) and electron microscopy is preferred to get the full insight to structure and dynamics of nanoparticles in our sample systems."

At present the NanoSight system is also used for educational purposes at Masaryk University (practical courses in Nanotechnology).

To learn more about particle characterization using Malvern's unique nanoparticle tracking analysis solutions, visit www.nanosight.com

####

About Malvern Instruments
Malvern provides the materials and biophysical characterization technology and expertise that enable scientists and engineers to understand and control the properties of dispersed systems. These systems range from proteins and polymers in solution, particle and nanoparticle suspensions and emulsions through to sprays and aerosols, industrial bulk powders and high concentration slurries. Used at all stages of research, development and manufacturing, Malvern's materials characterization instruments provide critical information that helps accelerate research and product development, enhance and maintain product quality and optimize process efficiency. Our products reflect Malvern's drive to exploit the latest technological innovations and our commitment to maximizing the potential of established techniques. They are used by both industry and academia, in sectors ranging from pharmaceuticals and biopharmaceuticals to bulk chemicals, cement, plastics and polymers, energy and the environment.

Malvern systems are used to measure particle size and count, particle shape, zeta potential, protein charge, molecular weight, mass, size and conformation, rheological properties and for chemical identification, advancing the understanding of dispersed systems across many different industries and applications.

Headquartered in Malvern, UK, Malvern Instruments has subsidiary organizations in all major European markets, North America, Mexico, China, Japan and Korea, a joint venture in India, a global distributor network and applications laboratories around the world. www.malvern.com.

About NTA

"Nanoparticle Tracking Analysis" (NTA) detects and visualizes populations of nanoparticles in liquids down to 10 nm, dependent on material, and measures the size of each particle from direct observations of diffusion. Additionally, NTA measures concentration and a fluorescence mode differentiates suitably-labelled particles within complex background suspensions. Zeta potential measurements are similarly particle-specific.

This real-time data gives insight into the kinetics of protein aggregation and other time-dependent phenomena in a qualitative and quantitative manner. NTA has a growing role in biodiagnostics, being proven in detection and speciation of extracellular vesicles.

For more information, please click here

Contacts:
NanoSight Limited
Minton Park
London Road
Amesbury SP4 7RT UK
T +44(0)1980 676060
F +44(0)1980 624703
www.nanosight.com


Talking Science Limited
39 de Bohun Court
Saffron Walden
Essex CB10 2BA UK
T +44(0)1799 521881
M +44(0)7843 012997
www.talking-science.com

Copyright © Malvern Instruments

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

A new, tunable device for spintronics: An international team of scientists including physicist Jairo Sinova from the University of Mainz realises a tunable spin-charge converter made of GaAs August 29th, 2014

Nanoscale assembly line August 29th, 2014

New analytical technology reveals 'nanomechanical' surface traits August 29th, 2014

New Vice President Takes Helm at CNSE CMOST: Catherine Gilbert To Lead CNSE Children’s Museum of Science and Technology Through Expansion And Relocation August 29th, 2014

Nanomedicine

Nanoscale assembly line August 29th, 2014

Copper shines as flexible conductor August 29th, 2014

Novel 'butterfly' molecule could build new sensors, photoenergy conversion devices August 28th, 2014

PetLife Comments on CNN Story on Scorpion Venom Health Benefits August 27th, 2014

Discoveries

A new, tunable device for spintronics: An international team of scientists including physicist Jairo Sinova from the University of Mainz realises a tunable spin-charge converter made of GaAs August 29th, 2014

Nanoscale assembly line August 29th, 2014

Copper shines as flexible conductor August 29th, 2014

Novel 'butterfly' molecule could build new sensors, photoenergy conversion devices August 28th, 2014

Announcements

A new, tunable device for spintronics: An international team of scientists including physicist Jairo Sinova from the University of Mainz realises a tunable spin-charge converter made of GaAs August 29th, 2014

Nanoscale assembly line August 29th, 2014

New analytical technology reveals 'nanomechanical' surface traits August 29th, 2014

New Vice President Takes Helm at CNSE CMOST: Catherine Gilbert To Lead CNSE Children’s Museum of Science and Technology Through Expansion And Relocation August 29th, 2014

Tools

New analytical technology reveals 'nanomechanical' surface traits August 29th, 2014

Ultra-Low Frequency Vibration Isolation Stabilizes Scanning Tunneling Microscopy at UCLA’s Nano-Research Group August 28th, 2014

Measure Both Elastic and Viscous Properties with AFM Using Asylum Research’s Exclusive AM-FM Viscoelastic Mapping Mode August 28th, 2014

Malvern specialists to deliver inaugural short course on polymer characterization at Interplas 2014 August 27th, 2014

Research partnerships

Leading European communications companies and research organizations have launched an EU project developing the future 5th Generation cellular mobile networks August 28th, 2014

New technique uses fraction of measurements to efficiently find quantum wave functions August 28th, 2014

The thunder god vine, assisted by nanotechnology, could shake up future cancer treatment: Targeted therapy for hepatocellular carcinoma using nanotechnology August 27th, 2014

Competition for Graphene: Berkeley Lab Researchers Demonstrate Ultrafast Charge Transfer in New Family of 2D Semiconductors August 26th, 2014

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE