Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Wyatt Technology’s FFF-MALS Provides Efficient Liposome Characterization

Abstract:
Wyatt's field flow fractionation (FFF) technology combined with multi-angle light scattering (MALS) and quasi-elastic light scattering (QELS) enables rapid measurements of particle size, size distribution, particle count, as well as structure.

Wyatt Technology’s FFF-MALS Provides Efficient Liposome Characterization

Santa Barbara, CA | Posted on August 19th, 2011



Wyatt Technology Corporation, the world leader in instrumentation for absolute macromolecular characterization and software, announces that its field flow fractionation (FFF) technology combined with multi-angle light scattering (MALS) and quasi-elastic light scattering (QELS) enables rapid measurements of particle size, size distribution, particle count, as well as structure. The combined system performs the most difficult sizing tasks in key areas of research and development in molecular biology and nanotechnology analyses, including the separation and characterization of liposomes and nanoparticles. This novel technology is illustrated in a new application note, titled "Liposome Characterization by FFF-MALS-QELS", which is available to download free-of charge via the website.

Liposomes are made of lipid bilayers. The size of a liposome ranges from some 20 nm up to several micrometers and may be composed of one or several concentric membranes. Liposomes possess unique properties owing to the amphiphilic character of the lipids, which make them suitable for drug delivery. Liposomes have attracted considerable attention as potential vehicles for drug delivery to selected cells or tissues in vivo. Therefore, it is of great importance to monitor liposome size and encapsulation during liposome research, formulation, manufacturing and quality control. However, conventional methods to characterize liposomes fail to perform efficiently and to cover the whole size of liposomes.

In this new application note, a method combining the Wyatt Technology Eclipse FFF system with a DAWN HELEOS and online QELS was used to analyze two liposome samples, one empty, and one filled. The results demonstrated that the combined method is capable of determining the degrees of encapsulation and the internal structures of the two liposomes without making assumptions.

The FFF system offers a number of distinct advantages over column separation techniques for many applications. The method is robust and easy to use, involving minimal shearing and enabling a broad separation range, from small proteins (nm) to large particles (µm). FFF is a non-destructive technique that achieves true particle size distribution and on-line absolute characterization of a wide range of solutions and dispersions. Combined with MALS detectors, the method can determine the absolute size and molar mass distributions as well as the structure and conformation of the particles.

For more information on Wyatt Technology's instruments or to obtain a copy of the new application note, please visit the website or email info[.]wyatt.com.

####

About Wyatt Technology
Based in Santa Barbara, California, Wyatt Technology (wyatt.com) is the world’s leading provider of instruments for absolute macromolecular characterization. With over 40 years’ experience developing multi-angle light scattering detectors, working with customers in the biotechnology, chemical, petrochemical, pharmaceutical, academic and government arenas, Wyatt prides itself on its entrepreneurial spirit, and the uniqueness of its offerings. The Company’s groundbreaking technology and uncompromising levels of customer care make Wyatt the global hallmark in its field.

For more information, please click here

Contacts:
Laura Browne
ScottPR.com
+44 1477 539539
wyatt[.]scottpr.com

Copyright © Newswire Today

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Making nanowires from protein and DNA September 3rd, 2015

Making fuel from light: Argonne research sheds light on photosynthesis and creation of solar fuel September 3rd, 2015

Reversible Writing with Light: Self-assembling nanoparticles take their cues from their surroundings September 3rd, 2015

Phagraphene, a 'relative' of graphene, discovered September 2nd, 2015

Nanomedicine

Making nanowires from protein and DNA September 3rd, 2015

Reversible Writing with Light: Self-assembling nanoparticles take their cues from their surroundings September 3rd, 2015

Silk bio-ink could help advance tissue engineering with 3-D printers September 2nd, 2015

Using DNA origami to build nanodevices of the future September 1st, 2015

Announcements

Making nanowires from protein and DNA September 3rd, 2015

Making fuel from light: Argonne research sheds light on photosynthesis and creation of solar fuel September 3rd, 2015

Reversible Writing with Light: Self-assembling nanoparticles take their cues from their surroundings September 3rd, 2015

A marine creature's magic trick explained: Crystal structures on the sea sapphire's back appear differently depending on the angle of reflection September 2nd, 2015

Tools

Oxford Instruments’ Triton Cryofree dilution refrigerator selected by Oxford University for developing scalable quantum nanodevices September 2nd, 2015

JEOL Introduces New Best-in-Class Field Emission SEM September 2nd, 2015

Atomic Force Microscopes from Asylum Research Guide the Development of Thin Film Deposition and Etch Processes September 2nd, 2015

Nanolab Technologies LEAPS Forward with High-Performance Analysis Services to the World: Nanolab Orders Advanced Local Electrode Atom Probe (LEAP®) Microscope from CAMECA Unit of AMETEK Materials Analysis Division August 27th, 2015

Nanobiotechnology

Making nanowires from protein and DNA September 3rd, 2015

A marine creature's magic trick explained: Crystal structures on the sea sapphire's back appear differently depending on the angle of reflection September 2nd, 2015

Using DNA origami to build nanodevices of the future September 1st, 2015

Researchers use DNA 'clews' to shuttle CRISPR-Cas9 gene-editing tool into cells August 30th, 2015

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







Car Brands
Buy website traffic