Nanotechnology Now







Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Johns Hopkins University uses NanoSight to study self-assembled polymer/DNA particles

Nupura Bhise of the Jordan group at JHU using the NanoSight LM10
Nupura Bhise of the Jordan group at JHU using the NanoSight LM10

Abstract:
The Department of Biomedical Engineering, Johns Hopkins University School of Medicine is using the NanoSight LM10-HS system to study self-assembled polymer/DNA particles for drug delivery.

Johns Hopkins University uses NanoSight to study self-assembled polymer/DNA particles

Salisbury, UK | Posted on October 29th, 2010

The main research interests of Dr Jordan Green of the Biomaterials and Drug Delivery Laboratory are in cellular engineering and nanobiotechnology. Knowledge of particle size is of particular value in the characterization of different drug delivery systems. Having had previous experience using dynamic light scattering techniques, Dr Green and his team now also use the complementary technique of nanoparticle tracking analysis from NanoSight. NTA provides insight into their samples particularly those with polydisperse behavior.

The laboratory chose the NanoSight LM10-HS system equipped with an EMCCD high sensitivity camera and a 404 nm laser for particle sizing analysis. In a typical study, particle solutions were diluted in DI water to adjust the sample concentration to a level such that there were approximately 30-60 light scattering centers in the visual analysis window. A sixty second movie containing the Brownian motion tracking of each individual particle was recorded. The movie was processed to enable detection of a least 250 particle tracks per sample. The NTA analysis gives a direct number-averaged distribution of the particle size as well as absolute particle concentration. The mean, standard deviation and mode of the particles is then calculated.

In this method, each individual particle is independently sized so that a direct number-averaged mean can be calculated. As each particle is counted, a mode, or the peak in the number distribution, can also be calculated. For monodisperse particle populations, both DLS and NTA measured the same values for particle size. However, NTA allowed finer distinction between peaks in samples that were polydisperse. The uniformity of polymeric nanoparticle distribution is thought to be a property of the polymer structure, and in particular, of the polymer terminal group. Changes to polymer terminal group were also found to dramatically change gene delivery efficacy of these nanoparticles in 2-D and 3-D cell systems.

Dr Green said that "To our knowledge, this is the first time that NTA has been used for self-assembled polymer/DNA particles. Our results highlight its utility, especially when combined with traditional DLS analysis."

To learn more about nanoparticle characterization using Nanoparticle Tracking Analysis, NTA, please visit the company website (www.nanosight.com) and register for the latest issue of NanoTrail, the company's electronic newsletter.

Reference:

This work was reported in the paper by N.S. Bhise et al. in Biomaterials 31 (2010) pp 8088 to 8096

####

About NanoSight
NanoSight Limited, of Salisbury, UK, provides unique nanoparticle characterization technology. “Nanoparticle Tracking Analysis” (NTA) detects and visualizes populations of nanoparticles in liquids down to 10nm (material dependent) and measures the size of each particle from direct observations of diffusion. This particle-by-particle methodology goes beyond traditional light scattering techniques such as Dynamic Light Scattering (DLS), or Photon Correlation Spectroscopy (PCS), in providing high-resolution particle size distributions. Additionally NanoSight measures concentration and validates all data with video of particles moving under Brownian motion.

This characterization information is highly informative in understanding the more complex suspensions in biological systems, hence its wide application in development of drug delivery systems, viral vaccines, the study of toxicology of nanoparticles and their environmental fate and in biomarker detection. This real-time data also provides insight into the kinetics of protein aggregation and other time-dependent phenomena in a quantitative manner, at deeply sub-micron sizes.

NanoSight has more than 250 systems installed worldwide with users including BASF, BP, GlaxoSmithKline, Novartis, 3M Corp., Roche, Solvay and Unilever together with many universities and research institutes. There are currently 100+ third party papers citing NanoSight results, with this reference base growing very rapidly as NanoSight consolidates its key contribution to nanoparticle characterization. For more information, visit the NanoSight website (www.nanosight.com).

For more information, please click here

Contacts:
NetDyaLog Limited
39 de Bohun Court
Saffron Walden
Essex CB10 2BA
T +44 (0) 1799 521881
www.netdyalog.com

Copyright © NanoSight

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

French Institutes IRT Nanoelec and CMP Team up to Offer World’s First Service for Post-process 3D Technologies on Multi-Project-Wafer March 5th, 2015

The George Washington University Opens Science and Engineering Hall, Largest Building of Its Kind in D.C.: Building Represents Significant Investment in Research Programs and Facilities; Commitment to Solve Global Problems, Improve Lives of Millions March 5th, 2015

Anousheh Ansari Wins the National Space Society's Space Pioneer Award for "Service to the Space Community" March 5th, 2015

Enhanced Graphene Components for Next Generation Racing Yacht March 5th, 2015

Academic/Education

Get ready for NanoDays! March 5th, 2015

NanoTecNexus Launches New App for Learning About Nanotechnology—STEM Education Project Spearheaded by Interns February 26th, 2015

SUNY Poly CNSE Researchers and Corporate Partners to Present Forty Papers at Globally Recognized Lithography Conference: SUNY Poly CNSE Research Group Awarded Both ‘Best Research Paper’ and ‘Best Research Poster’ at SPIE Advanced Lithography 2015 forum February 25th, 2015

KIT Increases Commitment in Asia: DAAD Funds Two New Projects: Strategic Partnerships with Chinese Universities and Communi-cation Technologies Network February 22nd, 2015

Self Assembly

Nanotubes self-organize and wiggle: Evolution of a nonequilibrium system demonstrates MEPP February 10th, 2015

Engineering self-assembling amyloid fibers January 26th, 2015

Revealed: How bacteria drill into our cells and kill them December 2nd, 2014

Live Images from the Nano-cosmos: Researchers watch layers of football molecules grow November 5th, 2014

Nanomedicine

Patent for the Novel Cancer Therapies – Ceramide Nanoliposomes March 4th, 2015

Arrowhead to Present at 2015 Barclays Global Healthcare Conference March 4th, 2015

Democratizing synthetic biology: New method makes research cheaper, faster, and more accessible March 3rd, 2015

Pens filled with high-tech inks for do-it-yourself sensors March 3rd, 2015

Announcements

The George Washington University Opens Science and Engineering Hall, Largest Building of Its Kind in D.C.: Building Represents Significant Investment in Research Programs and Facilities; Commitment to Solve Global Problems, Improve Lives of Millions March 5th, 2015

Anousheh Ansari Wins the National Space Society's Space Pioneer Award for "Service to the Space Community" March 5th, 2015

Enhanced Graphene Components for Next Generation Racing Yacht March 5th, 2015

Get ready for NanoDays! March 5th, 2015

Tools

Keysight Technologies Shifts to Direct Sales of High-Performance Products in North America March 3rd, 2015

Forbidden quantum leaps possible with high-res spectroscopy March 2nd, 2015

International research partnership tricks the light fantastic March 2nd, 2015

Important step towards quantum computing: Metals at atomic scale March 2nd, 2015

Nanobiotechnology

Experiment and theory unite at last in debate over microbial nanowires: New model and experiments settle debate over metallic-like conductivity of microbial nanowires in bacterium March 4th, 2015

Untangling DNA with a droplet of water, a pipet and a polymer: With the 'rolling droplet technique,' a DNA-injected water droplet rolls like a ball over a platelet, sticking the DNA to the plate surface February 27th, 2015

Bacteria network for food: Bacteria connect to each other and exchange nutrients February 23rd, 2015

Building tailor-made DNA nanotubes step by step: New, block-by-block assembly method could pave way for applications in opto-electronics, drug delivery February 23rd, 2015

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2015 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE