Nanotechnology Now

Our NanoNews Digest Sponsors



Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > University of Leicester uses NanoSight to characterize marine viruses & bacteriophages

The University of Leicester’s Dr Martha Clokie with her NanoSight LM10 system
The University of Leicester’s Dr Martha Clokie with her NanoSight LM10 system

Abstract:
The Department of Infection, Immunity and Inflammation at the University of Leicester is using the NanoSight nanoparticle characterization system to count bacteriophage and marine viruses.

University of Leicester uses NanoSight to characterize marine viruses & bacteriophages

Salisbury, UK | Posted on June 8th, 2010

Dr. Martha Clokie is a lecturer in microbiology. Her interests are focussed on the ecology and molecular biology of bacteriophages and their relationship with bacterial hosts; ranging from bacterial pathogens to environmentally important cyanobacteria; exploiting bacteriophages and phage-derived products as an alternative to treating antibiotic resistant bacterial infections.

Dr. Clokie has been using the NanoSight system to study cyanobacteria and their viruses which involves the accurate enumeration of viral particles. Prior to the use of the NanoSight system, Dr. Clokie would use time-consuming plaque assays to count viruses, this involves relatively large volumes of cyanobacterial culture and takes up to four weeks to obtain results. Now using NanoSight's unique nanoparticle tracking analysis, she is able to produce data in minutes and with no culturing required. Data from the NanoSight appears to correlate well with data from conventional plaque assays. With the typical viruses in the 100-300nm range and marine samples already being in the concentration range of 106 - 107 parts/ml, this is utilizing the sweet spot of the NanoSight technology which tracks and counts light scattered particle by particle.

Of her results to date, Dr. Clokie says it looks to provide a very promising method with significant ease of use, time-saving advantages. "Being able to visualise the sample without dilution gives me confidence in the results. The NanoSight has also shown advantages over flow cytometry where signal to noise issues can sometimes be a problem for us. This speed of data collection will allow me to probe far more data than I could do otherwise and should really allow the cyanobacterial/phage dynamic to be probed."

To learn more about nanoparticle characterization using Nanoparticle Tracking Analysis (NTA), please visit the company's website (www.nanosight.com) and register for the latest issue of NanoTrail, the company's electronic newsletter.

####

About NanoSight
NanoSight Limited, of Salisbury, UK, provides unique nanoparticle characterization technology. “Nanoparticle Tracking Analysis” (NTA) detects and visualizes populations of nanoparticles in liquids down to 10nm (material dependent) and measures the size of each particle from direct observations of diffusion. This particle-by-particle methodology goes beyond traditional light scattering techniques such as Dynamic Light Scattering (DLS), or Photon Correlation Spectroscopy (PCS), in providing high-resolution particle size distributions. Additionally NanoSight measures concentration and validates all data with video of particles moving under Brownian motion.

This characterization information is highly informative in understanding the more complex suspensions in biological systems, hence its wide application in development of drug delivery systems, viral vaccines, the study of toxicology of nanoparticles and their environmental fate and in biomarker detection. This real-time data also provides insight into the kinetics of protein aggregation and other time-dependent phenomena in a quantitative manner, at deeply sub-micron sizes.

NanoSight has more than 250 systems installed worldwide with users including BASF, BP, GlaxoSmithKline, Novartis, 3M Corp., Roche, Solvay and Unilever together with many universities and research institutes. There are currently 100+ third party papers citing NanoSight results, with this reference base growing very rapidly as NanoSight consolidates its key contribution to nanoparticle characterization.

For more information, please click here

Contacts:
Jezz Leckenby
+44 (0) 1799 521881

Copyright © NanoSight

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

New research project supports internationalisation in nano-research: Launch of new “Baltic Sea Network” November 22nd, 2014

3rd Iran-Proposed Nano Standard Approved by International Standard Organization November 22nd, 2014

NMTI announces breakthrough solutions for HAMR nanoantenna for next-generation ultra-high density magnetic storage November 21st, 2014

Canatu Launches CNB In-Mold Film for Transparent Touch on 3D Surfaces –in Cars, Household Appliances, Wearables, Portables November 20th, 2014

Marine/Watercraft

BRAAVOO will design an unmanned surveying vessel and marine buoy that carry biosensors to monitor marine pollutants November 12th, 2014

First Observation of Electronic Structure in Ag-Rh Alloy Nanoparticles Having Hydrogen Absorbing: Storage Property –Attempting to solve the mystery of why Ag-Rh alloy nanoparticles have a similar property to Pd– October 30th, 2014

Engineered proteins stick like glue — even in water: New adhesives based on mussel proteins could be useful for naval or medical applications September 22nd, 2014

NRL Researchers Develop Harder Ceramic for Armor Windows April 29th, 2014

Announcements

New research project supports internationalisation in nano-research: Launch of new “Baltic Sea Network” November 22nd, 2014

3rd Iran-Proposed Nano Standard Approved by International Standard Organization November 22nd, 2014

NMTI announces breakthrough solutions for HAMR nanoantenna for next-generation ultra-high density magnetic storage November 21st, 2014

Nano Sorbents Able to Remove Pollutions Caused by Oil Derivatives November 20th, 2014

Tools

Leica Microsystems Presents Universal Hybrid Detector for Single Molecule Detection and Imaging at SfN and ASCB: Leica HyD SMD - the Optimal Detector for Precise and Reliable SMD data November 20th, 2014

Nanometrics Announces Upcoming Investor Events November 19th, 2014

Two sensors in one: Nanoparticles that enable both MRI and fluorescent imaging could monitor cancer, other diseases November 18th, 2014

Field-emission plug-and-play solution for microwave electron guns: To simplify the electron emission mechanism involved in microwave electron guns, a team of researchers has created and demonstrated a field-emission plug-and-play solution based on ultrananocrystalline diamond November 18th, 2014

New-Contracts/Sales/Customers

Tesla NanoCoatings Increasing Use of SouthWest NanoTechnologies Carbon Nanotubes (CNTs) for its Infrastructure Coatings and Paints: High Quality SMW™ Specialty Multi-wall Carbon Nanotubes Incorporated into Teslan®-brand coatings used by Transportation, Oil and Gas Companies November 19th, 2014

Artificial retina could someday help restore vision November 12th, 2014

Lomiko Signs Licensing Agreement to Produce and Supply Power Converter Systems to E-Commerce Customers October 29th, 2014

Haydale Secures Exclusive Development and Supply Agreement with Tantec A/S: New reactors to be built and commissioned by Tantec A/S represent another step forward towards the commercialisation of graphene October 24th, 2014

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More












ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE