Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > New microscopy technique reveals mechanics of blood cell membranes

Abstract:
Thanks to an interdisciplinary team of researchers, scientists now have a more complete understanding of one of the human body's most vital structures: the red blood cell.

New microscopy technique reveals mechanics of blood cell membranes

Champaign, IL | Posted on April 30th, 2010

Led by University of Illinois electrical and computer engineering professor Gabriel Popescu, the team developed a model that could lead to breakthroughs in screening and treatment of blood-cell-morphology diseases, such as malaria and sickle-cell disease. The group published its findings in the Proceedings of the National Academy of Sciences.

Red blood cells (RBCs) are unique in structure - a doughnut-shaped disc full of the oxygen-carrying molecule hemoglobin but none of the intracellular structures of other cells, not even DNA. In circulation, RBCs must contort to squeeze through capillaries half their diameter. Their flexibility and resilience come from their membrane structure, which couples a typical lipid bilayer with an underlying matrix of protein. However, knowledge of the membrane's mechanics is very limited.

"The deformability of red blood cells is their most important property," said Popescu, also affiliated with the Beckman Institute for Advanced Science and Technology at U. of I. "What we wanted to find is, how does deformability relate to morphology?"

The research team used a novel measurement technique called diffraction phase microscopy, which uses two beams of light while other microscopes only use one.

"One beam goes through the specimen and one beam is used as a reference," Popescu said. "It is very, very sensitive to minute displacements in the membrane, down to the nanoscale."

RBC membrane movement can be observed through typical light microscopes, a phenomenon known as "flickering," but Popescu's team was able not only to see nanoscale membrane fluctuations in live cells, but also to measure them quantitatively - a first.

In addition to normal cells, the team also measured two other morphologies: bumpy RBCs called echinocytes and round ones called spherocytes. They discovered that these deformed cells display less flexibility in their membranes, a finding that could provide insight into mechanics and treatment of diseases that affect RBC shape, such as malaria, sickle-cell disease and spherocytosis.

With collaborators from UCLA, the group used its data to construct a new model of the RBC membrane that accounts for fluctuations and curvature, a more complete and accurate rendering than previous models that treated the membrane as a flat sheet.

"Our measurements showed that a flat model could not explain the data. With this curvature model, we understand much better what is happening in the RBC," said Popescu, adding, "It's really a combination of a new optical method and new theoretical model, and that is what allowed us to find some new results where the shape and deformability are coupled."

The team's technique eventually could be used to screen for blood diseases such as malaria or to screen banked blood for membrane flexibility before transfusion, since stored blood often undergoes cellular shape changes.

In addition, this novel microscopy technique has important implications for researchers interested in membrane biology and dynamics, according to Catherine Best, co-author of the paper and instructor in the U. of I. College of Medicine. "An advantage to studying red blood cells in this way is that we can now look at the effects of chemical agents on membranes, specifically. It is very exciting. For instance, we can look at the membrane effects of alcohol, and we may learn something about tolerance to alcohol," Best said.

Because diffraction phase microscopy measures live cells without physically manipulating or damaging them, it also could be used to evaluate medications being developed to treat blood cell morphology diseases, according to Popescu. "We can study the mechanics of a single cell under different pharmacological conditions, and I think that would be ideal for testing drugs," he said.

The National Institutes of Health and the National Science Foundation funded this research, which included collaborators from MIT, Harvard Medical School, the University of Colorado, Harvard University and UCLA.

####

For more information, please click here

Contacts:
Liz Ahlberg
Physical Sciences Editor
217-244-1073


Gabriel Popescu

Copyright © University of Illinois at Urbana-Champaign

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Haydale Secures Exclusive Development and Supply Agreement with Tantec A/S: New reactors to be built and commissioned by Tantec A/S represent another step forward towards the commercialisation of graphene October 24th, 2014

QuantumWise guides the semiconductor industry towards the atomic scale October 24th, 2014

MEMS & Sensors Technology Showcase: Finalists Announced for MEMS Executive Congress US 2014 October 23rd, 2014

Nanoparticle technology triples the production of biogas October 23rd, 2014

Possible Futures

Imaging electric charge propagating along microbial nanowires October 20th, 2014

Superconducting circuits, simplified: New circuit design could unlock the power of experimental superconducting computer chips October 18th, 2014

Nanocoatings Market By Product Is Expected To Reach USD 8.17 Billion By 2020: Grand View Research, Inc. October 15th, 2014

Perpetuus Carbon Group Receives Independent Verification of its Production Capacity for Graphenes at 140 Tonnes per Annum: Perpetuus Becomes the First Manufacturer in the Sector to Allow Third Party Audit October 7th, 2014

Nanomedicine

NYU Researchers Break Nano Barrier to Engineer the First Protein Microfiber October 23rd, 2014

Iranian Scientists Apply Nanotechnology to Produce Surgery Suture October 23rd, 2014

RF Heating of Magnetic Nanoparticles Improves the Thawing of Cryopreserved Biomaterials October 23rd, 2014

Sopping up proteins with thermosponges: Researchers develop novel nanoparticle platform that proves effective in delivering protein-based drugs October 22nd, 2014

Announcements

Haydale Secures Exclusive Development and Supply Agreement with Tantec A/S: New reactors to be built and commissioned by Tantec A/S represent another step forward towards the commercialisation of graphene October 24th, 2014

QuantumWise guides the semiconductor industry towards the atomic scale October 24th, 2014

Advancing thin film research with nanostructured AZO: Innovnano’s unique and cost-effective AZO sputtering targets for the production of transparent conducting oxides October 23rd, 2014

Strengthening thin-film bonds with ultrafast data collection October 23rd, 2014

Tools

Haydale Secures Exclusive Development and Supply Agreement with Tantec A/S: New reactors to be built and commissioned by Tantec A/S represent another step forward towards the commercialisation of graphene October 24th, 2014

National Synchrotron Light Source II Achieves 'First Light' October 23rd, 2014

Advancing thin film research with nanostructured AZO: Innovnano’s unique and cost-effective AZO sputtering targets for the production of transparent conducting oxides October 23rd, 2014

NIST offers electronics industry 2 ways to snoop on self-organizing molecules October 22nd, 2014

Nanobiotechnology

NYU Researchers Break Nano Barrier to Engineer the First Protein Microfiber October 23rd, 2014

Nanoparticle technology triples the production of biogas October 23rd, 2014

Mechanism behind nature's sparkles revealed October 22nd, 2014

‘Designer’ nanodevice could improve treatment options for cancer sufferers October 22nd, 2014

NanoNews-Digest
The latest news from around the world, FREE





  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE