Nanotechnology Now

Our NanoNews Digest Sponsors


Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Switched Off

Take the tube: The title conjugate provides spatiotemporal information of ATP in living cells by a two-step fluorescence quenching mechanism. OxyLrin, which is generated enzymatically in the reaction between ATP and D-luciferin (Lrin), is adsorbed onto the nanotubes and quenches their fluorescence (see picture). The sensor is highly selective toward ATP.
Take the tube: The title conjugate provides spatiotemporal information of ATP in living cells by a two-step fluorescence quenching mechanism. OxyLrin, which is generated enzymatically in the reaction between ATP and D-luciferin (Lrin), is adsorbed onto the nanotubes and quenches their fluorescence (see picture). The sensor is highly selective toward ATP.

Abstract:
Sensitive, selective, and resolved in time and space: ATP detection in living cells with carbon nanotubes and luciferase

Switched Off

Weinheim, Germany | Posted on February 12th, 2010

All living cells require a fuel to function: adenosine triphosphate (ATP), the cell "gasoline". Detecting ATP within cells can help researchers observe energetic physiological processes, such as signal cascades or transport processes. Furthermore, ATP depletion is related to certain diseases, such as Parkinson's disease and ischemia (restricted blood flow within tissues). A team led by Michael S. Strano at the Massachusetts Institute of Technology in Cambridge (USA) has now developed a more sensitive, higher-resolution, and more robust method for the detection of ATP. As the scientists report in the journal Angewandte Chemie, the method is based on carbon nanotubes.

ATP is usually detected by means of the luciferase assay. Luciferases are enzymes that are used in fireflies and other bioluminescent organisms to produce light. They use oxygen to convert a substrate called luciferin into oxyluciferin, which then reacts further to produce light. Certain luciferases use ATP for their reactions. The luciferase assay currently in use is complex, time-consuming, and suffers from a poor signal-to-noise ratio.

The MIT team has now developed a variation of the luciferase protocol: They attached the luciferase to carbon nanotubes. In this form the enzyme is easily taken up by cells. In the presence of luciferin and ATP, oxyluciferin is formed as usual, which causes fluorescence. What is interesting in this case is that carbon nanotubes normally fluoresce in the near infrared (nIR) spectral region; however this is proportionately extinguished by the addition of ATP to the luciferase reaction. Why? "As it is formed, the product oxyluciferin attaches itself firmly to the nanotube," explains Strano. "Electrons are transferred from the nanotube to the oxyluciferin so that the carbon nanotube itself can no longer fluoresce." The reduction in nIR fluorescence is easy to detect and serves as an indicator of the ATP concentration.

"Our new sensor is very selective for ATP," continues Strano. "We were able to use it to observe the change in ATP concentration over time and space in a cell culture."

Author: Michael S. Strano, Massachusetts Institute of Technology, Cambridge (USA), web.mit.edu/stranogroup/

Title: A Luciferase/Single-walled Carbon Nanotube Conjugate for Near-Infrared Fluorescent Detection of Cellular ATP

Angewandte Chemie International Edition 2010, 49, No. 8, 1456-1459, Permalink: dx.doi.org/10.1002/anie.200906251

####

About Wiley InterScience
Wiley InterScience (www.interscience.wiley.com) provides access to over 3 million articles across nearly 1500 journals and 7000 Online Books and major reference works. It also holds industry leading databases such as The Cochrane Library, chemistry databases and the acclaimed Current Protocols laboratory manuals.

Wiley InterScience is one of the world's premiere resources for study, teaching and advanced research.

For more information, please click here

Contacts:
Editorial office


Amy Molnar (US)


Jennifer Beal (UK)


Alina Boey (Asia)

Copyright © Wiley InterScience

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Nanotechnology and math deliver two-in-one punch for cancer therapy resistance June 24th, 2016

Researchers discover new chemical sensing technique: Technique allows sharper detail -- and more information -- with near infrared light June 24th, 2016

GraphExeter illuminates bright new future for flexible lighting devices June 23rd, 2016

Soft decoupling of organic molecules on metal June 23rd, 2016

Nanotubes/Buckyballs/Fullerenes

Nanotubes' 'stuffing' as is: A scientist from the Lomonosov Moscow State University studied the types of carbon nanotubes' 'stuffing' June 2nd, 2016

Programmable materials find strength in molecular repetition May 23rd, 2016

Nanotubes are beacons in cancer-imaging technique: Rice University researchers use spectral triangulation to pinpoint location of tumors May 21st, 2016

Unveiling the electron's motion in a carbon nanocoil: Development of a precise resistivity measurement system for quasi-one-dimensional nanomaterials using a focused ion beam May 16th, 2016

Nanomedicine

Nanotechnology and math deliver two-in-one punch for cancer therapy resistance June 24th, 2016

Self-assembling icosahedral protein designed: Self-assembling icosahedral protein designed June 22nd, 2016

Stealth nanocapsules kill Chagas parasites in mouse models June 22nd, 2016

New nanoparticle technology developed to treat aggressive thyroid cancer: Platform designed to deliver nanotherapy effective in preclinical models of metastatic anaplastic thyroid cancer June 21st, 2016

Sensors

Researchers discover new chemical sensing technique: Technique allows sharper detail -- and more information -- with near infrared light June 24th, 2016

Artificial synapse rivals biological ones in energy consumption June 21st, 2016

A new form of hybrid photodetectors with quantum dots and graphene June 19th, 2016

Drum beats from a one atom thick graphite membrane June 15th, 2016

Announcements

Nanotechnology and math deliver two-in-one punch for cancer therapy resistance June 24th, 2016

Researchers discover new chemical sensing technique: Technique allows sharper detail -- and more information -- with near infrared light June 24th, 2016

GraphExeter illuminates bright new future for flexible lighting devices June 23rd, 2016

Soft decoupling of organic molecules on metal June 23rd, 2016

Nanobiotechnology

Nanotechnology and math deliver two-in-one punch for cancer therapy resistance June 24th, 2016

Tailored DNA shifts electrons into the 'fast lane': DNA nanowire improved by altering sequences June 22nd, 2016

Self-assembling icosahedral protein designed: Self-assembling icosahedral protein designed June 22nd, 2016

Stealth nanocapsules kill Chagas parasites in mouse models June 22nd, 2016

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







Car Brands
Buy website traffic