Nanotechnology Now

Our NanoNews Digest Sponsors



Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Switched Off

Take the tube: The title conjugate provides spatiotemporal information of ATP in living cells by a two-step fluorescence quenching mechanism. OxyLrin, which is generated enzymatically in the reaction between ATP and D-luciferin (Lrin), is adsorbed onto the nanotubes and quenches their fluorescence (see picture). The sensor is highly selective toward ATP.
Take the tube: The title conjugate provides spatiotemporal information of ATP in living cells by a two-step fluorescence quenching mechanism. OxyLrin, which is generated enzymatically in the reaction between ATP and D-luciferin (Lrin), is adsorbed onto the nanotubes and quenches their fluorescence (see picture). The sensor is highly selective toward ATP.

Abstract:
Sensitive, selective, and resolved in time and space: ATP detection in living cells with carbon nanotubes and luciferase

Switched Off

Weinheim, Germany | Posted on February 12th, 2010

All living cells require a fuel to function: adenosine triphosphate (ATP), the cell "gasoline". Detecting ATP within cells can help researchers observe energetic physiological processes, such as signal cascades or transport processes. Furthermore, ATP depletion is related to certain diseases, such as Parkinson's disease and ischemia (restricted blood flow within tissues). A team led by Michael S. Strano at the Massachusetts Institute of Technology in Cambridge (USA) has now developed a more sensitive, higher-resolution, and more robust method for the detection of ATP. As the scientists report in the journal Angewandte Chemie, the method is based on carbon nanotubes.

ATP is usually detected by means of the luciferase assay. Luciferases are enzymes that are used in fireflies and other bioluminescent organisms to produce light. They use oxygen to convert a substrate called luciferin into oxyluciferin, which then reacts further to produce light. Certain luciferases use ATP for their reactions. The luciferase assay currently in use is complex, time-consuming, and suffers from a poor signal-to-noise ratio.

The MIT team has now developed a variation of the luciferase protocol: They attached the luciferase to carbon nanotubes. In this form the enzyme is easily taken up by cells. In the presence of luciferin and ATP, oxyluciferin is formed as usual, which causes fluorescence. What is interesting in this case is that carbon nanotubes normally fluoresce in the near infrared (nIR) spectral region; however this is proportionately extinguished by the addition of ATP to the luciferase reaction. Why? "As it is formed, the product oxyluciferin attaches itself firmly to the nanotube," explains Strano. "Electrons are transferred from the nanotube to the oxyluciferin so that the carbon nanotube itself can no longer fluoresce." The reduction in nIR fluorescence is easy to detect and serves as an indicator of the ATP concentration.

"Our new sensor is very selective for ATP," continues Strano. "We were able to use it to observe the change in ATP concentration over time and space in a cell culture."

Author: Michael S. Strano, Massachusetts Institute of Technology, Cambridge (USA), web.mit.edu/stranogroup/

Title: A Luciferase/Single-walled Carbon Nanotube Conjugate for Near-Infrared Fluorescent Detection of Cellular ATP

Angewandte Chemie International Edition 2010, 49, No. 8, 1456-1459, Permalink: dx.doi.org/10.1002/anie.200906251

####

About Wiley InterScience
Wiley InterScience (www.interscience.wiley.com) provides access to over 3 million articles across nearly 1500 journals and 7000 Online Books and major reference works. It also holds industry leading databases such as The Cochrane Library, chemistry databases and the acclaimed Current Protocols laboratory manuals.

Wiley InterScience is one of the world's premiere resources for study, teaching and advanced research.

For more information, please click here

Contacts:
Editorial office


Amy Molnar (US)


Jennifer Beal (UK)


Alina Boey (Asia)

Copyright © Wiley InterScience

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Effective Nanotechnology Innovations to Receive Mustafa Prize September 16th, 2014

‘Small’ transformation yields big changes September 16th, 2014

Elusive Quantum Transformations Found Near Absolute Zero: Brookhaven Lab and Stony Brook University researchers measured the quantum fluctuations behind a novel magnetic material's ultra-cold ferromagnetic phase transition September 15th, 2014

'Squid skin' metamaterials project yields vivid color display: Rice lab creates RGB color display technology with aluminum nanorods September 15th, 2014

Nanotubes/Buckyballs

‘Small’ transformation yields big changes September 16th, 2014

Rice rolls 'neat' nanotube fibers: Rice University researchers' acid-free approach leads to strong conductive carbon threads September 15th, 2014

Berkeley Lab Licenses Boron Nitride Nanotube Technology: New material has unique mechanical and electronic properties September 13th, 2014

Global Energy Systems Signs Master Sales Agreement with China Aviation Supplies Group September 4th, 2014

Nanomedicine

Treatment of Cell Infection by Nanotechnology September 15th, 2014

Researchers Create World’s Largest DNA Origami September 11th, 2014

Iranian Scientists Discover Nanotechnology Method to Remove Limitations in Tumor Surgery September 11th, 2014

Iranian Nanotechnology Scientists Produce Polymeric Scaffolds for Tissue Engineering September 11th, 2014

Sensors

Simple, Cost-Effective Method Proposed for Synthesizing Zinc Oxide Nanopigments September 15th, 2014

First Colloid and Polymer Science Lecture awarded to Orlin D. Velev: Chemical engineer honored for outstanding research in colloid science September 12th, 2014

UT Arlington research uses nanotechnology to help cool electrons with no external sources September 11th, 2014

Development of Algorithm for Accurate Calculation of Average Distance Travelled by Low-Speed Electrons without Energy Loss that Are Sensitive to Surface Structure September 11th, 2014

Announcements

Effective Nanotechnology Innovations to Receive Mustafa Prize September 16th, 2014

‘Small’ transformation yields big changes September 16th, 2014

Rice rolls 'neat' nanotube fibers: Rice University researchers' acid-free approach leads to strong conductive carbon threads September 15th, 2014

Simple, Cost-Effective Method Proposed for Synthesizing Zinc Oxide Nanopigments September 15th, 2014

Nanobiotechnology

NanoStruck has a High Recovery Rate on Mine Tailings: retrieval of up to 96% of Gold, 88% of Silver and 86% of Palladium September 12th, 2014

Boosting armor for nuclear-waste eating microbes September 12th, 2014

Researchers Create World’s Largest DNA Origami September 11th, 2014

UO-Berkeley Lab unveil new nano-sized synthetic scaffolding technique: Oil-and-water approach from Richmond's UO lab to spark new line of versatile peptoid nanosheets September 2nd, 2014

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE