Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > Scientists watch as peptides control crystal growth with ‘switches, throttles and brakes’

Aspartic acid-rich peptides adsorbed on a calcium oxalate monohydrate (COM) crystal surface. Models of a peptide and COM crystal structure are overlain on an AFM image collected during growth of the [010] face of COM. The rendered AFM image reveals the molecular structure of the crystal surface including individual kink-sites along a single atomic step. The high peaks show the individual oxalate groups and the dimension along the bottom edge of the image is 60 angstroms.
Aspartic acid-rich peptides adsorbed on a calcium oxalate monohydrate (COM) crystal surface. Models of a peptide and COM crystal structure are overlain on an AFM image collected during growth of the [010] face of COM. The rendered AFM image reveals the molecular structure of the crystal surface including individual kink-sites along a single atomic step. The high peaks show the individual oxalate groups and the dimension along the bottom edge of the image is 60 angstroms.

Abstract:
By producing some of the highest resolution images of peptides attaching to mineral surfaces, scientists have a deeper understanding how biomolecules manipulate the growth crystals. This research may lead to a new treatment for kidney stones using biomolecules.

Scientists watch as peptides control crystal growth with ‘switches, throttles and brakes’

Livermore, CA | Posted on November 25th, 2009

The research, which appears in the Nov. 23 online edition of the journal Proceedings of the National Academy of Science, explores how peptides interact with mineral surfaces by accelerating, switching and inhibiting their growth.

The team, made up of researchers from Lawrence Livermore National Laboratory, the Molecular Foundry at Lawrence Berkeley, the University of California, Davis and the University of Alabama, for the first time produced single-molecule resolution images of this peptide-mineral interaction.

Inorganic minerals play an important role in most biological organisms. Bone, teeth, protective shells or the intricate cell walls of marine diatoms are some displays of biomineralization, where living organisms form structures using inorganic material. Some minerals also can have negative effects on an organism such as in kidney and gallstones, which lead to severe suffering and internal damage in humans and other mammals.

Understanding how organisms limit the growth of pathological inorganic minerals is important in developing new treatment strategies. But deciphering the complex pathways that organisms use to create strong and versatile structures from relatively simple materials is no easy feat. To better understand the process, scientists attempt to mimic them in the laboratory.

By improving the resolution power of an Atomic Force Microscope (AFM), the PNAS authors were able to image individual atomic layers of the crystal interacting with small protein fragments, or peptides, as they fell on the surface of the crystal.

"Imaging biomolecules that are weakly attached to a surface, while simultaneously achieving single-molecule resolution, is normally difficult to do without knocking the molecules off," said Raymond Friddle, an LLNL postdoctoral fellow.

But the team improved upon previous methods and achieved unprecedented resolution of the molecular structure of the crystal surface during the dynamic interaction of each growing layer with peptides. "We were able to watch peptides adhere to the surface, temporarily slow down a layer of the growing crystal, and surprisingly ‘hop' to the next level of the crystal surface."

The images also revealed a mechanism that molecules can use to bind to surfaces that would normally repel them. The high resolution images showed that peptides will cluster together on crystal faces that present the same electronic charge. Under certain conditions the peptides would slow down growth, while under other conditions the peptides could speed up growth.

On another face of the crystal, where the peptides were expected to bind strongly, the researchers found instead that the peptides did not attach to the surface unless the crystal growth slowed. The peptides needed to bind in a specific way to the face, which takes more time than a non-specific attachment. As a result, the growing layers of the crystal were able to shed off the peptides as they attempted to bind.

But when the researchers slowed down the crystal growth rate, the peptides collapsed onto the surface so strongly that they completely stopped growth. The researchers proposed that the phenomenon is due to the unique properties of bio-polymers, such as peptides or polyelectrolytes, which fluctuate in solution before resting in a stable configuration on a surface.

"The results of the catastrophic drop in growth by peptides suggest ways that organisms achieve protection against pathological mineralization," said Jim De Yoreo, the project lead and deputy director of research at LBNL's Molecular Foundry. "Once growth is halted, a very high concentration of the mineral will be needed before growth can again reach significant levels."

He said designing polyelectrolyte modifiers in which the charge, size and ability to repel water can be systematically varied would allow researchers to create the equivalent of "switches, throttles and brakes" for directing crystallization.

####

About Lawrence Livermore National Laboratory
Founded in 1952, Lawrence Livermore National Laboratory is a national security laboratory, with a mission to ensure national security and apply science and technology to the important issues of our time. Lawrence Livermore National Laboratory is managed by Lawrence Livermore National Security, LLC for the U.S. Department of Energy's National Nuclear Security Administration.

For more information, please click here

Contacts:
Anne M. Stark
(925) 422-9799

Copyright © Lawrence Livermore National Laboratory

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

GLOBALFOUNDRIES and Chengdu Partner to Expand FD-SOI Ecosystem in China: More than $100M investment to establish a center of excellence for FDXTM FD-SOI design May 23rd, 2017

Zap! Graphene is bad news for bacteria: Rice, Ben-Gurion universities show laser-induced graphene kills bacteria, resists biofouling May 22nd, 2017

Leti Will Demo World’s-first WVGA 10-µm Pitch GaN Microdisplays for Augmented Reality Video at Display Week in Los Angles: Invited Paper also Will Present Leti’s Success with New Augmented Reality Technology That Reduces Pixel Pitch to Less than 5 Microns May 22nd, 2017

Graphene-nanotube hybrid boosts lithium metal batteries: Rice University prototypes store 3 times the energy of lithium-ion batteries May 19th, 2017

Possible Futures

GLOBALFOUNDRIES and Chengdu Partner to Expand FD-SOI Ecosystem in China: More than $100M investment to establish a center of excellence for FDXTM FD-SOI design May 23rd, 2017

Zap! Graphene is bad news for bacteria: Rice, Ben-Gurion universities show laser-induced graphene kills bacteria, resists biofouling May 22nd, 2017

Leti Will Demo World’s-first WVGA 10-µm Pitch GaN Microdisplays for Augmented Reality Video at Display Week in Los Angles: Invited Paper also Will Present Leti’s Success with New Augmented Reality Technology That Reduces Pixel Pitch to Less than 5 Microns May 22nd, 2017

Graphene-nanotube hybrid boosts lithium metal batteries: Rice University prototypes store 3 times the energy of lithium-ion batteries May 19th, 2017

Nanomedicine

Zap! Graphene is bad news for bacteria: Rice, Ben-Gurion universities show laser-induced graphene kills bacteria, resists biofouling May 22nd, 2017

Sensors detect disease markers in breath May 19th, 2017

Oddball enzyme provides easy path to synthetic biomaterials May 17th, 2017

The brighter side of twisted polymers: Conjugated polymers designed with a twist produce tiny, brightly fluorescent particles with broad applications May 16th, 2017

Announcements

GLOBALFOUNDRIES and Chengdu Partner to Expand FD-SOI Ecosystem in China: More than $100M investment to establish a center of excellence for FDXTM FD-SOI design May 23rd, 2017

Zap! Graphene is bad news for bacteria: Rice, Ben-Gurion universities show laser-induced graphene kills bacteria, resists biofouling May 22nd, 2017

Leti Will Demo World’s-first WVGA 10-µm Pitch GaN Microdisplays for Augmented Reality Video at Display Week in Los Angles: Invited Paper also Will Present Leti’s Success with New Augmented Reality Technology That Reduces Pixel Pitch to Less than 5 Microns May 22nd, 2017

Graphene-nanotube hybrid boosts lithium metal batteries: Rice University prototypes store 3 times the energy of lithium-ion batteries May 19th, 2017

Tools

Plasmon-powered upconversion nanocrystals for enhanced bioimaging and polarized emission: Plasmonic gold nanorods brighten lanthanide-doped upconversion superdots for improved multiphoton bioimaging contrast and enable polarization-selective nonlinear emissions for novel nanoscal May 19th, 2017

The brighter side of twisted polymers: Conjugated polymers designed with a twist produce tiny, brightly fluorescent particles with broad applications May 16th, 2017

Racyics Launches ‘makeChip’ Design Service Platform for GLOBALFOUNDRIES’ 22FDX® Technology: Racyics will provide IP and design services as a part of the foundry’s FDXcelerator™ Partner Program May 11th, 2017

UnitySC Announces Wafer Thinning Inspection System; Win from Power Semiconductor IDM for Automotive: Leading IDM Selects New 4See Series Automated Defect Inspection Platform for Power Semiconductor Automotive Applications May 11th, 2017

Nanobiotechnology

Zap! Graphene is bad news for bacteria: Rice, Ben-Gurion universities show laser-induced graphene kills bacteria, resists biofouling May 22nd, 2017

Sensors detect disease markers in breath May 19th, 2017

Oddball enzyme provides easy path to synthetic biomaterials May 17th, 2017

The brighter side of twisted polymers: Conjugated polymers designed with a twist produce tiny, brightly fluorescent particles with broad applications May 16th, 2017

Alliances/Trade associations/Partnerships/Distributorships

California Research Alliance by BASF establishes more than 25 research projects in three years April 26th, 2017

BASF and Landa partner to create revolutionary pigments for automotive coatings: The alliance combines BASF innovations with Landa nano-pigment technology April 5th, 2017

Leti Announces EU/South Korean Project for World’s First 5G-system Prototype: Coinciding with the 2018 Winter Games in PyeongChang, Korea, Prototype Will Be First Time State-of-the-art Terrestrial Wireless Communication Is Seamlessly Combined with Disruptive Satellite Communicati April 4th, 2017

ATTOPSEMI Technology Joins FDXcelerator Program to Deliver Advanced Non-Volatile Memory IP to GLOBALFOUNDRIES 22 FDX® Technology Platform: Leading-edge I-fuse™ brings higher reliability, smaller cell size and ease of programmability for consumer, automotive, and IoT applications March 27th, 2017

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project