Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Nanostructured Integrated Circuit Detects Type and Severity of Cancer

Abstract:
A team of investigators from the University of Toronto have used nanomaterials to develop an inexpensive microchip sensitive enough to quickly determine the type and severity of a patient's cancer so that the disease can be detected earlier for more effective treatment. Their work, reported in two papers published in the journals ACS Nano and Nature Nanotechnology, could herald an era when inexpensive yet sophisticated molecular diagnostics will become commonplace.

Nanostructured Integrated Circuit Detects Type and Severity of Cancer

Bethesda, MD | Posted on October 29th, 2009

The researchers' new device can readily detect the signature biomarkers that indicate the presence of cancer at the cellular level, even though these biomolecules - genes that indicate aggressive or benign forms of the disease and differentiate subtypes of the cancer - are generally present only at low levels in biological samples. Analysis can be completed in 90 minutes, a significant improvement over the existing diagnostic procedures that generally take days.

"Today, it takes a room filled with computers to evaluate a clinically relevant sample of cancer biomarkers and the results aren't quickly available," said team co-leader Shana Kelley. "Our team was able to measure biomolecules on an electronic chip the size of your fingertip and analyse the sample within half an hour. The instrumentation required for this analysis can be contained within a unit the size of a BlackBerry."

The nanoelectrode device that Kelley, collaborator Edward Sargent, and their students created is able to detect disease-related genes without the use of PCR to amplify low-level DNA. The electrodes, which are the key component of the device, have a novel highly-branched nanostructured shape that can detect attomolar concentrations of DNA. Using arrays of electrodes, each differing in the degree of nanostructured branching, the investigators were able to construct a device capable of sensing DNA molecules over six orders of magnitude, overcoming the dynamic range issue - the ability to detect both common and rare molecules - that has plagued other devices.

The investigators fabricated these devices using a standard microchip production process known as photolithography to create the basic electrode grid needed to measure multiple biomarkers simultaneously, and then used a second technique known as electrodeposition to grow the branched nanostructures on the electrodes, controlling the size of each electrode by varying the time over which electrodeposition occurred. With the electrodes in place, the investigators then coated them with various DNA-binding molecules known as peptide-nucleic acids, or PNAs, that can be designed to bind to a specific gene sequence. When a piece of DNA binds to its complementary DNA or RNA molecule, it triggers a chemical reaction that alters the electrical signal generated by the associated electrode.

Using their device, the investigators analyzed messenger RNA samples from prostate cancer biopsies. Their analysis showed that the device can detect gene fusions characteristic of prostate cancer. More importantly, the device was able to distinguish between gene fusions associated with either fast- or slow-growing forms of prostate cancer.

The paper describing the construction of this nanobiosensor is titled, "Programming the detection limits of biosensors through controlled nanostructuring." An abstract of this paper is available at the journal's Web site.

View abstract here www.nature.com/nnano/journal/vaop/ncurrent/abs/nnano.2009.276.html

The paper detailing the use of the nanobiosensor to detect and characterize cancers is titled, "Direct Profiling of Cancer Biomarkers in Tumor Tissue Using a Multiplexed Nanostructured Microelectrode Integrated Circuit." An abstract of this paper is available at the journal's Web site.

####

About NCI Alliance for Nanotechnology in Cancer
The NCI Alliance for Nanotechnology in Cancer is engaged in efforts to harness the power of nanotechnology to radically change the way we diagnose, treat, and prevent cancer. Through its programs and initiatives, the Alliance is committed to building a community of researchers dedicated to using nanotechnology to advance the fight against cancer.
As part of the Center for Strategic Scientific Initiatives which is led by NCI Deputy Director Dr. Anna Barker, the Alliance for Nanotechnology in Cancer works in concert with other NCI advanced technology initiatives to provide the scientific foundation and team science that is required to transform cancer research and care.

For more information, please click here

Contacts:
NCI Alliance for Nanotechnology in Cancer
Building 31, Room 10A52
31 Center Drive, MSC 2580
Bethesda, MD 20892-2580
(301) 496-1550

Copyright © NCI Alliance for Nanotechnology in Cancer

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Seeing quantum motion August 30th, 2015

Artificial leaf harnesses sunlight for efficient fuel production August 30th, 2015

Researchers use DNA 'clews' to shuttle CRISPR-Cas9 gene-editing tool into cells August 30th, 2015

Draw out of the predicted interatomic force August 30th, 2015

Possible Futures

Sediment dwelling creatures at risk from nanoparticles in common household products August 13th, 2015

Harris & Harris Group Reports Financial Statements as of June 30, 2015, and Announces a Stock Repurchase Program August 10th, 2015

Molecular trick alters rules of attraction for non-magnetic metals August 5th, 2015

Global Carbon Nanotubes Industry 2015: Acute Market Reports August 4th, 2015

Nanomedicine

Researchers use DNA 'clews' to shuttle CRISPR-Cas9 gene-editing tool into cells August 30th, 2015

Iranian Scientists Use Artemisia Annua Plant to Produce Breast Cancer Drugs August 29th, 2015

Small but heading for the big time: Nanobiotix half year results for the six months ended 30 June 2015, in line with expectations: Major clinical achievements and corporate developments August 28th, 2015

A new technique to make drugs more soluble August 28th, 2015

Nanoelectronics

Nanotechnology that will impact the Security & Defense sectors to be discussed at NanoSD2015 conference August 25th, 2015

'Quantum dot' technology may help light the future August 19th, 2015

Surprising discoveries about 2-D molybdenum disulfide: Berkeley Lab researchers use award-winning campanile probe on promising semiconductor August 15th, 2015

Better together: Graphene-nanotube hybrid switches August 3rd, 2015

Announcements

Seeing quantum motion August 30th, 2015

Artificial leaf harnesses sunlight for efficient fuel production August 30th, 2015

Researchers use DNA 'clews' to shuttle CRISPR-Cas9 gene-editing tool into cells August 30th, 2015

Draw out of the predicted interatomic force August 30th, 2015

Nanobiotechnology

Researchers use DNA 'clews' to shuttle CRISPR-Cas9 gene-editing tool into cells August 30th, 2015

Small but heading for the big time: Nanobiotix half year results for the six months ended 30 June 2015, in line with expectations: Major clinical achievements and corporate developments August 28th, 2015

Nanotechnology that will impact the Security & Defense sectors to be discussed at NanoSD2015 conference August 25th, 2015

Louisiana Tech University researchers discover synthesis of a new nanomaterial: Interdisciplinary team creates biocomposite for first time using physiological conditions August 24th, 2015

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







Car Brands
Buy website traffic