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Home > News > PALM microscopy enables understanding of cell organization's impact on biological function

July 14th, 2009

PALM microscopy enables understanding of cell organization's impact on biological function

Abstract:
Using PALM, Liphardt and his colleagues mapped the cellular locations of three proteins central to the chemotaxis signaling network--Tar, CheY and CheW--with a mean precision of 15 nanometers. They found that cluster sizes were distributed with no one size being "characteristic." For example, a third of the Tar proteins were part of smaller lateral clusters and not of the large polar clusters. Analysis of the relative cellular locations of more than one million individual proteins from 326 cells determined that they are not actively distributed or attached to specific locations in cells, as had been hypothesized.
"Instead," said Liphardt, "random lateral protein diffusion and protein-protein interactions are probably sufficient to generate the observed complex, ordered patterns. This simple stochastic self-assembly mechanism, which can create and maintain periodic structures in biological membranes without direct cytoskeletal involvement or active transport, may prove to be widespread in both prokaryotic and eukaryotic cells."

Source:
bioopticsworld.com

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