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October 30th, 2008
Scientists in Canada have recreated a famous painting on the microscale using a new protein patterning technique. The technique can also be used to mimic patterns of proteins found in cells and could lead to advances in neuroscience, they claim.
Santiago Costantino, at the University of Montreal, and colleagues used laser-assisted protein adsorption by photobleaching to create the protein patterns. He used a laser to bind a fluorescent compound called biotin-4-fluorescein (B4F) to a protein-coated glass surface. By moving the laser around and varying the intensity of the beam, he created patterns of B4F with varying thickness. He then bound other proteins and antibodies to the surface to create fluorescent and biologically active protein surfaces.
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