Nanotechnology Now

Our NanoNews Digest Sponsors
Heifer International



Home > Press > High-speed holographic fluorescence microscopy system with submicron resolution: The group has realized a scanless 3D imaging system and an algorithm for high-speed measurement

Overview of the developed high-speed holographic fluorescence microscopy system for scanless 3D measurement with submicron resolution.

CREDIT
National Institute of Information and Communications Technology (NICT), Tohoku University, Toin University of Yokohama, Japan Science and Technology Agency (JST)
Overview of the developed high-speed holographic fluorescence microscopy system for scanless 3D measurement with submicron resolution. CREDIT National Institute of Information and Communications Technology (NICT), Tohoku University, Toin University of Yokohama, Japan Science and Technology Agency (JST)

Abstract:
The National Institute of Information and Communications Technology (NICT), Tohoku University, Toin University of Yokohama, and Japan Science and Technology Agency (JST) have succeeded in developing a scanless high-speed holographic fluorescence microscopy system with submicron resolution for a 3D space. The system is based on digital holography. The developed microscopy system has an algorithm to acquire 3D information of fluorescent objects toward scanless 3D measurement in less than 1 millisecond. Scanless 3D sensing with submicron resolution and color-multiplexed holographic fluorescence imaging have been demonstrated using the algorithm. The microscopy system will be further developed to achieve holographic 3D motion-picture sensing of specimens with incoherent light.

This achievement was published in Optics Letters as an open-access paper on January 29, 2021.

High-speed holographic fluorescence microscopy system with submicron resolution: The group has realized a scanless 3D imaging system and an algorithm for high-speed measurement

Tokyo, Japan | Posted on January 29th, 2021

[Achievements]

The scanless high-speed holographic fluorescence microscopy system shown in Figure 1 was constructed. The system is based on digital holography and is applicable to the sensing of incoherent light such as fluorescence light and natural light. The developed algorithm enables the adoption of a phase modulator to generate two phase values, which is expected to increase the measurement speed. Submicron resolution for a 3D space was successfully demonstrated using fluorescent objects with a diameter of 0.2 micron. The experimental results shown in Figure 2 indicate that the developed microscopy system achieves 3D sensing of nanoparticles and has submicron resolution quantitatively for a 3D space. Scanless 3D measurement in less than 1 millisecond is achievable by using the algorithm with either a ferroelectric liquid crystal on silicon (FLCOS) or an electro-optic (EO) device. Color-multiplexed holographic fluorescence imaging with the algorithm and only four exposures has also been demonstrated by combining the proposed algorithm and computational coherent superposition (CCS). The number of exposures is reduced by the algorithm, and the number of photons per hologram is increased even for ultimately weak light.

[Future prospects]

High-speed holographic motion-picture imaging for 3D dynamics and multiple moving objects in a 3D space.
Improvements of the system such as recording of a quantitative phase, sensing of ultimately weak light, and construction of a compact optical setup.

####

For more information, please click here

Contacts:
HIROTA Sachiko

Copyright © National Institute of Information and Communications Technology (NICT)

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

RELATED JOURNAL ARTICLE:

Related News Press

News and information

Built to last: New copolymer binder to extend the life of lithium ion batteries: Scientists develop a novel binder material that protects the graphite anode of Li-ion batteries from degradation even after 1700 cycles March 5th, 2021

A COSMIC approach to nanoscale science: Instrument at Berkeley Lab's Advanced Light Source achieves world-leading resolution of nanomaterials March 5th, 2021

Light in concert with force reveals how materials become harder when illuminated: When indented by a probe in darkness, wafers of some semiconductors are putty-like. When illuminated by light whose wavelength matches the band gap, they become hard, as electrons and holes freed by March 5th, 2021

Taking 2D materials for a spin: Scientists at the University of Tsukuba and the Institute of High Pressure Physics fabricate a novel molybdenum disulfide transistor and create an image of the spins of the electrons passing through which may open the way for new spintronic compute March 5th, 2021

Imaging

How photoblueing disturbs microscopy February 26th, 2021

Engineering the boundary between 2D and 3D materials: Cutting-edge microscope helps reveal ways to control the electronic properties of atomically thin materials February 26th, 2021

Novel Flexible Terahertz Camera Can Inspect Objects with Diverse Shapes February 17th, 2021

Adaptive optics with cascading corrective elements: A cascaded dual deformable phase plate wavefront modulator enables direct AO integration with existing microscopes--doubling the aberration correction range and greatly improving image quality January 22nd, 2021

Possible Futures

Built to last: New copolymer binder to extend the life of lithium ion batteries: Scientists develop a novel binder material that protects the graphite anode of Li-ion batteries from degradation even after 1700 cycles March 5th, 2021

A COSMIC approach to nanoscale science: Instrument at Berkeley Lab's Advanced Light Source achieves world-leading resolution of nanomaterials March 5th, 2021

Light in concert with force reveals how materials become harder when illuminated: When indented by a probe in darkness, wafers of some semiconductors are putty-like. When illuminated by light whose wavelength matches the band gap, they become hard, as electrons and holes freed by March 5th, 2021

Taking 2D materials for a spin: Scientists at the University of Tsukuba and the Institute of High Pressure Physics fabricate a novel molybdenum disulfide transistor and create an image of the spins of the electrons passing through which may open the way for new spintronic compute March 5th, 2021

Discoveries

Built to last: New copolymer binder to extend the life of lithium ion batteries: Scientists develop a novel binder material that protects the graphite anode of Li-ion batteries from degradation even after 1700 cycles March 5th, 2021

A COSMIC approach to nanoscale science: Instrument at Berkeley Lab's Advanced Light Source achieves world-leading resolution of nanomaterials March 5th, 2021

Light in concert with force reveals how materials become harder when illuminated: When indented by a probe in darkness, wafers of some semiconductors are putty-like. When illuminated by light whose wavelength matches the band gap, they become hard, as electrons and holes freed by March 5th, 2021

Taking 2D materials for a spin: Scientists at the University of Tsukuba and the Institute of High Pressure Physics fabricate a novel molybdenum disulfide transistor and create an image of the spins of the electrons passing through which may open the way for new spintronic compute March 5th, 2021

Announcements

Built to last: New copolymer binder to extend the life of lithium ion batteries: Scientists develop a novel binder material that protects the graphite anode of Li-ion batteries from degradation even after 1700 cycles March 5th, 2021

A COSMIC approach to nanoscale science: Instrument at Berkeley Lab's Advanced Light Source achieves world-leading resolution of nanomaterials March 5th, 2021

Light in concert with force reveals how materials become harder when illuminated: When indented by a probe in darkness, wafers of some semiconductors are putty-like. When illuminated by light whose wavelength matches the band gap, they become hard, as electrons and holes freed by March 5th, 2021

Taking 2D materials for a spin: Scientists at the University of Tsukuba and the Institute of High Pressure Physics fabricate a novel molybdenum disulfide transistor and create an image of the spins of the electrons passing through which may open the way for new spintronic compute March 5th, 2021

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers/Posters

Built to last: New copolymer binder to extend the life of lithium ion batteries: Scientists develop a novel binder material that protects the graphite anode of Li-ion batteries from degradation even after 1700 cycles March 5th, 2021

A COSMIC approach to nanoscale science: Instrument at Berkeley Lab's Advanced Light Source achieves world-leading resolution of nanomaterials March 5th, 2021

Light in concert with force reveals how materials become harder when illuminated: When indented by a probe in darkness, wafers of some semiconductors are putty-like. When illuminated by light whose wavelength matches the band gap, they become hard, as electrons and holes freed by March 5th, 2021

Taking 2D materials for a spin: Scientists at the University of Tsukuba and the Institute of High Pressure Physics fabricate a novel molybdenum disulfide transistor and create an image of the spins of the electrons passing through which may open the way for new spintronic compute March 5th, 2021

Tools

From microsaws to nanodrills: laser pulses act as subtle machining tools: Industrial-grade materials processing on the sub-micron scale is enabled by spatially structured ultrashort laser pulses February 26th, 2021

Engineering the boundary between 2D and 3D materials: Cutting-edge microscope helps reveal ways to control the electronic properties of atomically thin materials February 26th, 2021

Novel Flexible Terahertz Camera Can Inspect Objects with Diverse Shapes February 17th, 2021

CEA Is the First Research Center to Acquire A Cryogenic Prober for Testing Quantum Bits February 10th, 2021

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project