Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > Researchers revolutionize vital conservation tool with use of gold nanotechnology and lasers: Cryopreservation study results have sweeping implications for wildlife conservation and human health

The research uses new gold nanotechnology and lasers to warm the embryo-the stumbling block in previous studies. The results have profound implications for human health, wildlife conservation, and aquaculture.
CREDIT
University of Minnesota/Smithsonian Conservation Biology Institute
The research uses new gold nanotechnology and lasers to warm the embryo-the stumbling block in previous studies. The results have profound implications for human health, wildlife conservation, and aquaculture. CREDIT University of Minnesota/Smithsonian Conservation Biology Institute

Abstract:
For more than 60 years, researchers have tried to successfully cryopreserve (or freeze) the embryo of zebrafish, a species that is an important medical model for human health. In a new study, researchers at the University of Minnesota and the Smithsonian Conservation Biology Institute (SCBI) provide the first-ever reproducible evidence for the successful cryopreservation of zebrafish embryos.

Researchers revolutionize vital conservation tool with use of gold nanotechnology and lasers: Cryopreservation study results have sweeping implications for wildlife conservation and human health

Minneapolis, MN | Posted on July 15th, 2017

The study uses new gold nanotechnology and lasers to warm the embryo--the stumbling block in previous studies. The results have profound implications for human health, wildlife conservation, and aquaculture.

The research is published today in ACS Nano, a leading scientific journal published by the American Chemical Society.

"There's no doubt that the use of this technology, in this way, marks a paradigm shift for cryopreservation and the conservation of many wildlife species," said Mary Hagedorn, an SCBI research scientist and paper co-author who has been working on cryopreserving zebrafish embryos since 1992.

"To get anything to work at such cold temperatures, you usually have to get creative. Here we take a unique approach by combining biology with an exciting engineering technology to do what has been impossible previously: to successfully freeze and thaw a fish embryo so that the embryo begins to develop, rather than falls apart," Hagedorn added.

By freezing sperm, eggs and embryos, conservationists can safeguard at-risk species and their genetic diversity, making it possible to bolster the genetic pool and therefore health of wild populations years--or even centuries--later. Although scientists have successfully cryopreserved the embryos of many mammal species and the sperm of many species of fish, freezing fish embryos proved infinitely more complicated.

Successful cryopreservation of an embryo requires cooling the embryo to a cryogenically stable state, then warming it at a rate faster than it was cooled, and using an antifreeze (or cryoprotectant) to stop the growth of ice crystals, which are like pins in a balloon that pop the membrane and cause the embryo to fall apart. Fish embryos, however, are very large, making it difficult to thaw them quickly and avoid ice crystal development. In addition, because aquatic animals need to survive harsh environments, their embryonic membranes are mostly impenetrable, blocking the cryoprotectants out.

Enter laser gold nanotechnology, a rapidly growing technological field being developed for cryopreservation applications by University of Minnesota Mechanical Engineering John Bischof that was critical for the success of the study and has a wide variety of biomedical applications.

"Lasers have the exciting ability to act like a "light switch" that can turn biological activity on and off within gold nanoparticle laden biomaterials," said Bischof, senior author of the study. "In this case, by careful engineering and deployment of gold nanoparticles within a cryogenically stored and biological inactive embryo, we can use a laser pulse to quickly warm the embryo back to ambient temperatures and switch biological activity, and therefore life, back on."

Gold nanorods are tiny cylinders of gold that convert absorbed light (from a laser, for example) into heat. The study's authors injected both the cryoprotectant and nanogold particles into the embryos. The gold particles transferred heat uniformly throughout the embryo when hit with a laser, warming the embryo from -196 degrees C to 20 degrees C in just one thousandth of a second. The amazingly fast warming rate, in combination with the cryoprotectant, prevented the formation of lethal ice crystals.

Embryos that underwent this process went on to develop at least to the 24-hour stage where they developed a heart, gills, tail musculature and moved--proving their post-thaw viability.

The study's authors next aim to fine-tune the process to ensure that they can increase the survival rate of the embryos. They will also investigate the use of automation to bolster how many embryos they can successfully thaw at once.

Because the embryos of other aquatic animals--fish, amphibians and coral--are very similar to those of zebrafish, this technology is directly applicable to the cryopreservation of many species' embryos. The technology may also be customized to cryopreserve reptile and bird embryos and enhance the process of cryopreserving mammalian embryos, including giant pandas and large cats. In addition, the technology can help aquaculture farms become more efficient and cost effective, putting less pressure on wild populations.

Human health researchers use zebrafish--which have a genome similar to that of humans--as important disease models to study melanoma, heart disease and blood disorders, among other health issues. Cryopreserved zebrafish embryos will prevent the scientists from losing entire research lines and will give them the flexibility to bring the lines back as needed.

###

In addition to Hagedorn and Bischof, the lead authors of the paper are University of Minnesota Ph.D. students Kanav Khosla and Yiru Wang with help from former University of Minnesota Ph.D. student Zhenpeng Qin.

####

For more information, please click here

Contacts:
Rhonda Zurn

612-626-7959

Copyright © University of Minnesota

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

To read the full research paper, visit the ACS Nano website:

Related News Press

News and information

Carbon nanotube optics poised to provide pathway to optical-based quantum cryptography and quantum computing: Researchers are exploring enhanced potential of carbon nanotubes for unique applications June 18th, 2018

Camouflaged nanoparticles used to deliver killer protein to cancer June 17th, 2018

Squeezing light at the nanoscale: Ultra-confined light could detect harmful molecules June 17th, 2018

Physicists devise method to reveal how light affects materials: The new method adds to the understanding of the fundamental laws governing the interaction of electrons and light June 15th, 2018

Govt.-Legislation/Regulation/Funding/Policy

Carbon nanotube optics poised to provide pathway to optical-based quantum cryptography and quantum computing: Researchers are exploring enhanced potential of carbon nanotubes for unique applications June 18th, 2018

Camouflaged nanoparticles used to deliver killer protein to cancer June 17th, 2018

Squeezing light at the nanoscale: Ultra-confined light could detect harmful molecules June 17th, 2018

Tripling the Energy Storage of Lithium-Ion Batteries: Scientists have synthesized a new cathode material from iron fluoride that surpasses the capacity limits of traditional lithium-ion batteries June 14th, 2018

Possible Futures

Carbon nanotube optics poised to provide pathway to optical-based quantum cryptography and quantum computing: Researchers are exploring enhanced potential of carbon nanotubes for unique applications June 18th, 2018

Camouflaged nanoparticles used to deliver killer protein to cancer June 17th, 2018

Squeezing light at the nanoscale: Ultra-confined light could detect harmful molecules June 17th, 2018

Physicists devise method to reveal how light affects materials: The new method adds to the understanding of the fundamental laws governing the interaction of electrons and light June 15th, 2018

Nanomedicine

Camouflaged nanoparticles used to deliver killer protein to cancer June 17th, 2018

Graphene carpets: So neurons communicate better: Research by SISSA reveals that graphene can strengthen neuronal activity, confirming the unique properties of this nanomaterial. The study has been published on Nature Nanotechnology June 13th, 2018

New optical sensor can determine if molecules are left or right 'handed' June 13th, 2018

A nanotech sensor that turns molecular fingerprints into bar codes June 7th, 2018

Discoveries

Carbon nanotube optics poised to provide pathway to optical-based quantum cryptography and quantum computing: Researchers are exploring enhanced potential of carbon nanotubes for unique applications June 18th, 2018

Camouflaged nanoparticles used to deliver killer protein to cancer June 17th, 2018

Squeezing light at the nanoscale: Ultra-confined light could detect harmful molecules June 17th, 2018

Physicists devise method to reveal how light affects materials: The new method adds to the understanding of the fundamental laws governing the interaction of electrons and light June 15th, 2018

Announcements

Carbon nanotube optics poised to provide pathway to optical-based quantum cryptography and quantum computing: Researchers are exploring enhanced potential of carbon nanotubes for unique applications June 18th, 2018

Camouflaged nanoparticles used to deliver killer protein to cancer June 17th, 2018

Squeezing light at the nanoscale: Ultra-confined light could detect harmful molecules June 17th, 2018

Physicists devise method to reveal how light affects materials: The new method adds to the understanding of the fundamental laws governing the interaction of electrons and light June 15th, 2018

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

Carbon nanotube optics poised to provide pathway to optical-based quantum cryptography and quantum computing: Researchers are exploring enhanced potential of carbon nanotubes for unique applications June 18th, 2018

Camouflaged nanoparticles used to deliver killer protein to cancer June 17th, 2018

Squeezing light at the nanoscale: Ultra-confined light could detect harmful molecules June 17th, 2018

Physicists devise method to reveal how light affects materials: The new method adds to the understanding of the fundamental laws governing the interaction of electrons and light June 15th, 2018

Environment

Squeezing light at the nanoscale: Ultra-confined light could detect harmful molecules June 17th, 2018

A nanotech sensor that turns molecular fingerprints into bar codes June 7th, 2018

Engineered polymer membranes could be new option for water treatment May 6th, 2018

Harvesting clean hydrogen fuel through artificial photosynthesis May 3rd, 2018

Grants/Sponsored Research/Awards/Scholarships/Gifts/Contests/Honors/Records

Squeezing light at the nanoscale: Ultra-confined light could detect harmful molecules June 17th, 2018

SUNY Poly Professor Eric Lifshin Selected for ‘Fellow of the Microanalysis Society’ Position for Significant Contributions to Microanalysis June 13th, 2018

New optical sensor can determine if molecules are left or right 'handed' June 13th, 2018

Evidence for a new property of quantum matter revealed: Electrical dipole activity detected in a quantum material unlike any other tested June 11th, 2018

Nanobiotechnology

Camouflaged nanoparticles used to deliver killer protein to cancer June 17th, 2018

Graphene carpets: So neurons communicate better: Research by SISSA reveals that graphene can strengthen neuronal activity, confirming the unique properties of this nanomaterial. The study has been published on Nature Nanotechnology June 13th, 2018

New optical sensor can determine if molecules are left or right 'handed' June 13th, 2018

Promising news from biomedicine: DNA origami more resilient than previously understood June 4th, 2018

Photonics/Optics/Lasers

Carbon nanotube optics poised to provide pathway to optical-based quantum cryptography and quantum computing: Researchers are exploring enhanced potential of carbon nanotubes for unique applications June 18th, 2018

Squeezing light at the nanoscale: Ultra-confined light could detect harmful molecules June 17th, 2018

New optical sensor can determine if molecules are left or right 'handed' June 13th, 2018

Leti Presenting Strategic Vision and Hosting a Workshop at SEMICON West: “From Electrons to Photons” Leti Workshop and CEO Media Briefing Set for Tuesday, July 10 in W Hotel, San Francisco June 12th, 2018

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project