Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button

Home > Press > Researchers revolutionize vital conservation tool with use of gold nanotechnology and lasers: Cryopreservation study results have sweeping implications for wildlife conservation and human health

The research uses new gold nanotechnology and lasers to warm the embryo-the stumbling block in previous studies. The results have profound implications for human health, wildlife conservation, and aquaculture.
CREDIT
University of Minnesota/Smithsonian Conservation Biology Institute
The research uses new gold nanotechnology and lasers to warm the embryo-the stumbling block in previous studies. The results have profound implications for human health, wildlife conservation, and aquaculture. CREDIT University of Minnesota/Smithsonian Conservation Biology Institute

Abstract:
For more than 60 years, researchers have tried to successfully cryopreserve (or freeze) the embryo of zebrafish, a species that is an important medical model for human health. In a new study, researchers at the University of Minnesota and the Smithsonian Conservation Biology Institute (SCBI) provide the first-ever reproducible evidence for the successful cryopreservation of zebrafish embryos.

Researchers revolutionize vital conservation tool with use of gold nanotechnology and lasers: Cryopreservation study results have sweeping implications for wildlife conservation and human health

Minneapolis, MN | Posted on July 15th, 2017

The study uses new gold nanotechnology and lasers to warm the embryo--the stumbling block in previous studies. The results have profound implications for human health, wildlife conservation, and aquaculture.

The research is published today in ACS Nano, a leading scientific journal published by the American Chemical Society.

"There's no doubt that the use of this technology, in this way, marks a paradigm shift for cryopreservation and the conservation of many wildlife species," said Mary Hagedorn, an SCBI research scientist and paper co-author who has been working on cryopreserving zebrafish embryos since 1992.

"To get anything to work at such cold temperatures, you usually have to get creative. Here we take a unique approach by combining biology with an exciting engineering technology to do what has been impossible previously: to successfully freeze and thaw a fish embryo so that the embryo begins to develop, rather than falls apart," Hagedorn added.

By freezing sperm, eggs and embryos, conservationists can safeguard at-risk species and their genetic diversity, making it possible to bolster the genetic pool and therefore health of wild populations years--or even centuries--later. Although scientists have successfully cryopreserved the embryos of many mammal species and the sperm of many species of fish, freezing fish embryos proved infinitely more complicated.

Successful cryopreservation of an embryo requires cooling the embryo to a cryogenically stable state, then warming it at a rate faster than it was cooled, and using an antifreeze (or cryoprotectant) to stop the growth of ice crystals, which are like pins in a balloon that pop the membrane and cause the embryo to fall apart. Fish embryos, however, are very large, making it difficult to thaw them quickly and avoid ice crystal development. In addition, because aquatic animals need to survive harsh environments, their embryonic membranes are mostly impenetrable, blocking the cryoprotectants out.

Enter laser gold nanotechnology, a rapidly growing technological field being developed for cryopreservation applications by University of Minnesota Mechanical Engineering John Bischof that was critical for the success of the study and has a wide variety of biomedical applications.

"Lasers have the exciting ability to act like a "light switch" that can turn biological activity on and off within gold nanoparticle laden biomaterials," said Bischof, senior author of the study. "In this case, by careful engineering and deployment of gold nanoparticles within a cryogenically stored and biological inactive embryo, we can use a laser pulse to quickly warm the embryo back to ambient temperatures and switch biological activity, and therefore life, back on."

Gold nanorods are tiny cylinders of gold that convert absorbed light (from a laser, for example) into heat. The study's authors injected both the cryoprotectant and nanogold particles into the embryos. The gold particles transferred heat uniformly throughout the embryo when hit with a laser, warming the embryo from -196 degrees C to 20 degrees C in just one thousandth of a second. The amazingly fast warming rate, in combination with the cryoprotectant, prevented the formation of lethal ice crystals.

Embryos that underwent this process went on to develop at least to the 24-hour stage where they developed a heart, gills, tail musculature and moved--proving their post-thaw viability.

The study's authors next aim to fine-tune the process to ensure that they can increase the survival rate of the embryos. They will also investigate the use of automation to bolster how many embryos they can successfully thaw at once.

Because the embryos of other aquatic animals--fish, amphibians and coral--are very similar to those of zebrafish, this technology is directly applicable to the cryopreservation of many species' embryos. The technology may also be customized to cryopreserve reptile and bird embryos and enhance the process of cryopreserving mammalian embryos, including giant pandas and large cats. In addition, the technology can help aquaculture farms become more efficient and cost effective, putting less pressure on wild populations.

Human health researchers use zebrafish--which have a genome similar to that of humans--as important disease models to study melanoma, heart disease and blood disorders, among other health issues. Cryopreserved zebrafish embryos will prevent the scientists from losing entire research lines and will give them the flexibility to bring the lines back as needed.

###

In addition to Hagedorn and Bischof, the lead authors of the paper are University of Minnesota Ph.D. students Kanav Khosla and Yiru Wang with help from former University of Minnesota Ph.D. student Zhenpeng Qin.

####

For more information, please click here

Contacts:
Rhonda Zurn

612-626-7959

Copyright © University of Minnesota

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

To read the full research paper, visit the ACS Nano website:

Related News Press

News and information

Organic solar cells reach record efficiency, benchmark for commercialization April 23rd, 2018

Remote-control shoots laser at nano-gold to turn on cancer-killing immune cells April 20th, 2018

New qubit now works without breaks: A universal design for superconducting qubits has been created April 19th, 2018

Observing biological nanotransporters: Chemistry April 19th, 2018

Govt.-Legislation/Regulation/Funding/Policy

Organic solar cells reach record efficiency, benchmark for commercialization April 23rd, 2018

Remote-control shoots laser at nano-gold to turn on cancer-killing immune cells April 20th, 2018

Salt boosts creation of 2-D materials: Rice University scientists show how salt lowers reaction temperatures to make novel materials April 18th, 2018

Quantum shift shows itself in coupled light and matter: Rice University scientists corral, quantify subtle movement in condensed matter system April 16th, 2018

Possible Futures

Organic solar cells reach record efficiency, benchmark for commercialization April 23rd, 2018

Remote-control shoots laser at nano-gold to turn on cancer-killing immune cells April 20th, 2018

New qubit now works without breaks: A universal design for superconducting qubits has been created April 19th, 2018

Observing biological nanotransporters: Chemistry April 19th, 2018

Nanomedicine

Remote-control shoots laser at nano-gold to turn on cancer-killing immune cells April 20th, 2018

Observing biological nanotransporters: Chemistry April 19th, 2018

Nanobiotix Shows NBTXR3 Nanoparticles Can Stoke Anti-Tumor Immune Response April 17th, 2018

Tiny nanomachine successfully completes test drive: Researchers at the University of Bonn and the research institute Caesar build a one-wheeled vehicle out of DNA rings April 11th, 2018

Discoveries

Organic solar cells reach record efficiency, benchmark for commercialization April 23rd, 2018

Remote-control shoots laser at nano-gold to turn on cancer-killing immune cells April 20th, 2018

New qubit now works without breaks: A universal design for superconducting qubits has been created April 19th, 2018

Observing biological nanotransporters: Chemistry April 19th, 2018

Announcements

Organic solar cells reach record efficiency, benchmark for commercialization April 23rd, 2018

Remote-control shoots laser at nano-gold to turn on cancer-killing immune cells April 20th, 2018

New qubit now works without breaks: A universal design for superconducting qubits has been created April 19th, 2018

Observing biological nanotransporters: Chemistry April 19th, 2018

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

Organic solar cells reach record efficiency, benchmark for commercialization April 23rd, 2018

Remote-control shoots laser at nano-gold to turn on cancer-killing immune cells April 20th, 2018

New qubit now works without breaks: A universal design for superconducting qubits has been created April 19th, 2018

Observing biological nanotransporters: Chemistry April 19th, 2018

Environment

'Sweet spot' in sweet material for hydrogen storage: Study IDs 'white graphene' architecture with unprecedented hydrogen storage capacity March 12th, 2018

Converting CO2 into Usable Energy: Scientists show that single nickel atoms are an efficient, cost-effective catalyst for converting carbon dioxide into useful chemicals March 1st, 2018

Ultra-efficient removal of carbon monoxide using gold nanoparticles on a molecular support: New method and mechanism for state-of-the-art gas purification February 9th, 2018

New filters could enable manufacturers to perform highly-selective chemical separation January 23rd, 2018

Grants/Sponsored Research/Awards/Scholarships/Gifts/Contests/Honors/Records

Remote-control shoots laser at nano-gold to turn on cancer-killing immune cells April 20th, 2018

Lifeboat Foundation funds flying 3D-printed classroom cubesats with Perlan II April 16th, 2018

Thermo Scientific Krios G3i Cryo-Electron Microscope Wins Gold Edison Award: Krios G3i helps scientists better understand disease mechanisms in order to accelerate cures April 12th, 2018

Bloodless revolution in diabetes monitoring: Scientists have created a non-invasive, adhesive patch, which promises the measurement of glucose levels through the skin without a finger-prick blood test April 10th, 2018

Nanobiotechnology

Remote-control shoots laser at nano-gold to turn on cancer-killing immune cells April 20th, 2018

Observing biological nanotransporters: Chemistry April 19th, 2018

Nanobiotix Shows NBTXR3 Nanoparticles Can Stoke Anti-Tumor Immune Response April 17th, 2018

Tiny nanomachine successfully completes test drive: Researchers at the University of Bonn and the research institute Caesar build a one-wheeled vehicle out of DNA rings April 11th, 2018

Photonics/Optics/Lasers

Doing the nano-shimmy: New device modulates light and amplifies tiny signals April 12th, 2018

Phononic SEIRA -- enhancing light-molecule interactions via crystal lattice vibrations April 10th, 2018

High-speed and on-silicon-chip graphene blackbody emitters: Integrated light emitters for optical communications April 5th, 2018

Leti Silicon Photonics Design Kit Available in Synopsis OptoDesigner Suite: Kit Contains Design Rules and Building Blocks for Multi-Project Wafers And Custom Runs on Letiís Si310 Platform April 5th, 2018

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project