Nanotechnology Now

Our NanoNews Digest Sponsors
Heifer International



Home > Press > Droplets finally all the same size -- in a nanodroplet library

A microdroplet (here represented by a pomegranate) can be easily split into hundreds of easy-to-store nanodroplets (represented by seeds) -- with a simple microfluidic device that has been constructed at the Institute of Physical Chemistry of the Polish Academy of Sciences in Warsaw.
CREDIT:IPC PAS, Grzegorz Krzyzewski
A microdroplet (here represented by a pomegranate) can be easily split into hundreds of easy-to-store nanodroplets (represented by seeds) -- with a simple microfluidic device that has been constructed at the Institute of Physical Chemistry of the Polish Academy of Sciences in Warsaw.

CREDIT:IPC PAS, Grzegorz Krzyzewski

Abstract:
A single drop with the volume of a millionth of a litre is really not very large and certainly does not look like something you can do a lot with. However, a simple device, constructed at the Institute of Physical Chemistry of the Polish Academy of Sciences in Warsaw, can split the microdroplet into a collection of equally-sized nanodroplets. From now on, the valuable chemicals or genetic material contained in a single microdroplet can be the starting point of even hundreds of experiments - or they can be archived in the form of nanodroplet libraries.

Droplets finally all the same size -- in a nanodroplet library

Warsaw, Poland | Posted on June 20th, 2016

Until now, droplets produced by electronic pipettes or in sophisticated microfluidic devices differed in volume by a dozen or even several dozen percent or more. At the Institute of Physical Chemistry of the Polish Academy of Sciences (IPC PAS) in Warsaw a device has been developed that puts an end to inaccuracies and produces droplets of virtually identical volume, in addition they are calculated in nanolitres (billionth parts of a litre). Interestingly, the device is so simple that it can be used, for example, as the tip on a pipette. Its construction is an important step in the development of microfluidic systems, which are set to revolutionize chemistry just as integrated circuits did with electronics.

"In modern microfluidics droplets are produced by apparatus that is either more or less complex, in processes carefully controlled by computers. Our idea was different: we decided to transfer control of the formation of droplets not to devices, but to physics itself. However you look at it, physics still has several billion years more experience than we do in this field, doesn't it?" says Prof. Piotr Garstecki (IPC PAS).

Microfluidic systems are usually constructed by permanently sticking together two plates of transparent plastic (polycarbonate), where one of them is covered with a network of appropriately designed very thin grooves on the side that is stuck. After the plates are joined together, these grooves form channels into which a carrier liquid (usually oil) can be forced. By introducing a second liquid that is immiscible with the liquid carrier (e.g. a water solution) into the interior of a system that has been constructed in this manner, droplets can be produced, transported, split, combined and their contents mixed.

"The Holy Grail of microfluidics is to create a laboratory of a size similar to modern integrated circuits capable of carrying out complex chemical and biological experiments. In other words, a lab on a chip. This is the goal, because at the moment what we have is rather... a chip in a lab," says Dr. Filip Dutka (IPC PAS; Faculty of Physics, University of Warsaw) and explains - "Current microfluidic systems can already do a lot, their dimensions are small, but for such a plate to work, it has to be surrounded by syringe pumps, computers controlling flow, all in a tangle of tubes. Our device eliminates a large part of this cumbersome and expensive infrastructure".

The device, designed at the IPC PAS, commissioned by Curiosity Diagnostics, with the support of a grant from the Foundation for Polish Science and the European ERC Starting Grant, is none other than a channel with a carefully designed geometry. When the sample fluid is introduced from one side, it begins to pass through an outlet configured in such a way that when the right amount of liquid has passed through it, surface tension forces naturally close the surface. This happens at the same time on each occasion. As a result, each nanodroplet breaking off the outlet always has the same volume. Using different copies of the new instrument, Prof. Garstecki's group has produced series of droplets with sizes of from ca. 0.5 to ca. 50 nanolitres.

"Our device creates nanodroplets at a rate of several dozen per second, which is slightly slower than in traditional, controlled techniques. The number of droplets is smaller, but their quality has increased. Tests have shown that the size of the nanodroplets practically does not depend on the flow rate or viscosity of the sample liquid and a given version of the device always generates droplets of the same size. It's truly amazing that in terms of even a ten-fold difference in flow velocity, the volumes of the droplets differ from each other by only a few percent. This means that the user has complete freedom in the implementation of the experiment, and the result is always exactly in compliance with the designed protocol," says PhD student Adam Opalski, a biotechnologist from the IPC PAS.

The device developed at the IPC PAS has no moving parts, does not wear out and does not require power. Used in microfluidic systems, it will significantly reduce the need for their accompanying infrastructure, which should accelerate the dissemination of microfluidic equipment. Probably in a few years the device, patent pending, will also be widely available in the form of tips for a pipette.

Splitting even small amounts of liquid into nanodroplets opens up new research perspectives. Instead of one experiment on a microdroplet, it will now be possible to carry out several measurements in hundreds of separate experiments. The accuracy of statistical analysis will increase, and consequently the certainty of the results of laboratory and diagnostic tests. A particularly interesting field of application is related to the fact that the nanodroplets suspended in the liquid carrier do not have the tendency to merge. Using the microfluidic device from the IPC PAS the creation of a permanent and easy-to-store library of nanodroplets, numbering hundreds and thousands of droplets with valuable chemical or biological substances becomes child's play: all you need do is dip an electronic pipette with the appropriate tip in oil, press the button and ... wait.

####

About Institute of Physical Chemistry of the Polish Academy of Sciences
The Institute of Physical Chemistry of the Polish Academy of Sciences was established in 1955 as one of the first chemical institutes of the PAS. The Institute's scientific profile is strongly related to the newest global trends in the development of physical chemistry and chemical physics. Scientific research is conducted in nine scientific departments. CHEMIPAN R&D Laboratories, operating as part of the Institute, implement, produce and commercialise specialist chemicals to be used, in particular, in agriculture and pharmaceutical industry. The Institute publishes approximately 200 original research papers annually.

For more information, please click here

Contacts:
Piotr Garstecki

48-223-432-233

Copyright © Institute of Physical Chemistry of the Polish Academy of Sciences

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

RELATED JOURNAL ARTICLE:

Related News Press

Chemistry

What heat can tell us about battery chemistry: using the Peltier effect to study lithium-ion cells March 8th, 2024

Two-dimensional bimetallic selenium-containing metal-organic frameworks and their calcinated derivatives as electrocatalysts for overall water splitting March 8th, 2024

News and information

Researchers develop artificial building blocks of life March 8th, 2024

How surface roughness influences the adhesion of soft materials: Research team discovers universal mechanism that leads to adhesion hysteresis in soft materials March 8th, 2024

Two-dimensional bimetallic selenium-containing metal-organic frameworks and their calcinated derivatives as electrocatalysts for overall water splitting March 8th, 2024

Microfluidics/Nanofluidics

Implantable device shrinks pancreatic tumors: Taming pancreatic cancer with intratumoral immunotherapy April 14th, 2023

Computational system streamlines the design of fluidic devices: This computational tool can generate an optimal design for a complex fluidic device such as a combustion engine or a hydraulic pump December 9th, 2022

Researchers design new inks for 3D-printable wearable bioelectronics: Potential uses include printing electronic tattoos for medical tracking applications August 19th, 2022

Oregon State University research pushes closer to new therapy for pancreatic cancer May 6th, 2022

Possible Futures

Two-dimensional bimetallic selenium-containing metal-organic frameworks and their calcinated derivatives as electrocatalysts for overall water splitting March 8th, 2024

Curcumin nanoemulsion is tested for treatment of intestinal inflammation: A formulation developed by Brazilian researchers proved effective in tests involving mice March 8th, 2024

The Access to Advanced Health Institute receives up to $12.7 million to develop novel nanoalum adjuvant formulation for better protection against tuberculosis and pandemic influenza March 8th, 2024

Nanoscale CL thermometry with lanthanide-doped heavy-metal oxide in TEM March 8th, 2024

Nanomedicine

High-tech 'paint' could spare patients repeated surgeries March 8th, 2024

Researchers develop artificial building blocks of life March 8th, 2024

Curcumin nanoemulsion is tested for treatment of intestinal inflammation: A formulation developed by Brazilian researchers proved effective in tests involving mice March 8th, 2024

The Access to Advanced Health Institute receives up to $12.7 million to develop novel nanoalum adjuvant formulation for better protection against tuberculosis and pandemic influenza March 8th, 2024

Discoveries

What heat can tell us about battery chemistry: using the Peltier effect to study lithium-ion cells March 8th, 2024

Researchers’ approach may protect quantum computers from attacks March 8th, 2024

High-tech 'paint' could spare patients repeated surgeries March 8th, 2024

Nanoscale CL thermometry with lanthanide-doped heavy-metal oxide in TEM March 8th, 2024

Announcements

What heat can tell us about battery chemistry: using the Peltier effect to study lithium-ion cells March 8th, 2024

Curcumin nanoemulsion is tested for treatment of intestinal inflammation: A formulation developed by Brazilian researchers proved effective in tests involving mice March 8th, 2024

The Access to Advanced Health Institute receives up to $12.7 million to develop novel nanoalum adjuvant formulation for better protection against tuberculosis and pandemic influenza March 8th, 2024

Nanoscale CL thermometry with lanthanide-doped heavy-metal oxide in TEM March 8th, 2024

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers/Posters

Researchers develop artificial building blocks of life March 8th, 2024

How surface roughness influences the adhesion of soft materials: Research team discovers universal mechanism that leads to adhesion hysteresis in soft materials March 8th, 2024

Curcumin nanoemulsion is tested for treatment of intestinal inflammation: A formulation developed by Brazilian researchers proved effective in tests involving mice March 8th, 2024

Nanoscale CL thermometry with lanthanide-doped heavy-metal oxide in TEM March 8th, 2024

Nanobiotechnology

High-tech 'paint' could spare patients repeated surgeries March 8th, 2024

Researchers develop artificial building blocks of life March 8th, 2024

Curcumin nanoemulsion is tested for treatment of intestinal inflammation: A formulation developed by Brazilian researchers proved effective in tests involving mice March 8th, 2024

The Access to Advanced Health Institute receives up to $12.7 million to develop novel nanoalum adjuvant formulation for better protection against tuberculosis and pandemic influenza March 8th, 2024

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project