Nanotechnology Now

Our NanoNews Digest Sponsors
Heifer International



Home > Press > Microfluidic devices gently rotate small organisms and cells

This is the design and operation of the acoustofluidic rotational manipulation device. (a) A schematic of the experimental setup. The piezoelectric transducer that generates acoustic waves is placed adjacent to the microfluidic channel. The acoustic waves actuate air microbubbles trapped within sidewall microcavities. (b) An optical image showing a mid-L4 stage C. elegans trapped by multiple oscillating microbubbles. Scale bar = 100 micrometers.
CREDIT: Tony Huang, Penn State
This is the design and operation of the acoustofluidic rotational manipulation device. (a) A schematic of the experimental setup. The piezoelectric transducer that generates acoustic waves is placed adjacent to the microfluidic channel. The acoustic waves actuate air microbubbles trapped within sidewall microcavities. (b) An optical image showing a mid-L4 stage C. elegans trapped by multiple oscillating microbubbles. Scale bar = 100 micrometers.

CREDIT: Tony Huang, Penn State

Abstract:
A method to rotate single particles, cells or organisms using acoustic waves in a microfluidic device will allow researchers to take three dimensional images with only a cell phone. Acoustic waves can move and position biological specimens along the x, y and z axes, but for the first time researchers at Penn State have used them to gently and safely rotate samples, a crucial capability in single-cell analysis, drug discovery and organism studies.

Microfluidic devices gently rotate small organisms and cells

University Park, PA | Posted on March 24th, 2016

The research, published today in Nature Communications, was led by Tony Jun Huang, professor of engineering science and mechanics and Huck Distinguished Chair in Bioengineering Science. Huang and his group created an acoustofluidic rotational manipulation (ARM) method that traps bubbles in a series of small cavities inside a microfluidic device. Acoustic transducers similar to ultrasound imaging transducers create an acoustic wave in the fluid, making the bubbles vibrate, which creates microvortexes in the flowing liquid that are tunable so the sample rotates in any direction and at any desired speed.

"Currently confocal microscopes are required in many biological, biochemical and biomedical studies, but many labs do not have access to a confocal microscope, which costs more than $200,000," said Huang. "Our ARM method is a very inexpensive platform and it is compatible with all the optical characterization tools. You can literally use a cell phone to do three-dimensional imaging."

To demonstrate the device's capabilities, the researchers rotated C. elegans, a model organism about a millimeter in length frequently used in biological studies. They also acoustically rotated and imaged a HeLa cancer cell.

Existing methods of manipulating small objects depend on the optical, magnetic or electrical properties of the specimen, and/or damage the specimen due to laser heating. The ARM method, on the other hand, uses a gentle acoustic wave generated by a power similar to ultrasound imaging, and at a lower frequency. The device is also compact and simple to use.

"Our method is a valuable platform for imaging and studying the effect of rotation at the single cell level," said co-lead author Adem Ozceki, graduate student in engineering science and mechanics. "More important, with the capacity to rotate large numbers of cells in parallel, researchers will be able to perform high-throughput single-cell studies. "

In addition to its applicability to a large range of biological and physical science investigations, ARM technology shows excellent biocompatibility in a HeLa cell viability test in which 99.2 percent of cells survived manipulation.

###

Also contributing to "Rotational manipulation of single cells and organisms using acoustic waves" were former group member Daniel Ahmed, Ph.D.; graduate students Nagagireesh Bojanala, Nitesh Nama, Awani Upadhyay, Yuchao Chen; and Wendy Hanna-Rose, associate professor of biochemistry and molecular biology; all from Penn State. The National Institutes of Health; National Science Foundation; and the Center for Nanoscale Science, an NSF Materials Research Science and Engineering Center at Penn State supported this work. Components of the work were conducted at the Penn State Materials Research Institute's Nanofabrication Laboratory.

####

For more information, please click here

Contacts:
A'ndrea Elyse Messer

814-865-9481

Copyright © Penn State

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

New technology helps reveal inner workings of human genome June 24th, 2022

Advances in lithium niobate photonics: High performance integrated LN-based photonic devices have developed rapidly in recent years, and many different structures have been demonstrated for various application scenarios—are we about to enter a new era of LN photonics? June 24th, 2022

Advances in lithium niobate photonics: High performance integrated LN-based photonic devices have developed rapidly in recent years, and many different structures have been demonstrated for various application scenarios—are we about to enter a new era of LN photonics? June 24th, 2022

Boron nitride nanotube fibers get real: Rice lab creates first heat-tolerant, stable fibers from wet-spinning process June 24th, 2022

Microfluidics/Nanofluidics

Oregon State University research pushes closer to new therapy for pancreatic cancer May 6th, 2022

Imaging

Snapshot measurement of single nanostructure’s circular dichroism March 25th, 2022

Better understanding superconductors with Higgs spectroscopy Prof. Stefan Kaiser from TU Dresden awarded ERC Consolidator Grant March 18th, 2022

Turning any camera into a polarization camera: Metasurface attachment can be used with almost any optical system, from machine vision cameras to telescopes March 18th, 2022

Visualizing the invisible: New fluorescent DNA label reveals nanoscopic cancer features March 4th, 2022

Govt.-Legislation/Regulation/Funding/Policy

Solving the solar energy storage problem with rechargeable batteries that can convert and store energy at once June 24th, 2022

Boron nitride nanotube fibers get real: Rice lab creates first heat-tolerant, stable fibers from wet-spinning process June 24th, 2022

UBCO researchers change the game when it comes to activity tracking: Flexible, highly sensitive motion device created by extrusion printing June 17th, 2022

University of Illinois Chicago joins Brookhaven Lab's Quantum Center June 10th, 2022

Possible Futures

New technology helps reveal inner workings of human genome June 24th, 2022

Advances in lithium niobate photonics: High performance integrated LN-based photonic devices have developed rapidly in recent years, and many different structures have been demonstrated for various application scenarios—are we about to enter a new era of LN photonics? June 24th, 2022

Advances in lithium niobate photonics: High performance integrated LN-based photonic devices have developed rapidly in recent years, and many different structures have been demonstrated for various application scenarios—are we about to enter a new era of LN photonics? June 24th, 2022

Boron nitride nanotube fibers get real: Rice lab creates first heat-tolerant, stable fibers from wet-spinning process June 24th, 2022

Nanomedicine

From outside to inside: A rapid and precise total assessment method for cells: Researchers at Nara Institute of Science and Technology show that using four frequencies of applied voltage can improve the measurement of cell size and shape during impedance cytometry, enabling to en June 24th, 2022

New technology helps reveal inner workings of human genome June 24th, 2022

Disinfectant mechanism of nano-sized electrostatic atomized water particles on SARS-CoV-2: Nano-sized electrostatic atomized water particles destroy SARS-CoV-2 envelope, protein, and RNA, thereby impairing the virus’s ability to bind to host cells June 17th, 2022

New nano-gel to protect children receiving chemotherapy from hearing loss June 17th, 2022

Discoveries

New technology helps reveal inner workings of human genome June 24th, 2022

Advances in lithium niobate photonics: High performance integrated LN-based photonic devices have developed rapidly in recent years, and many different structures have been demonstrated for various application scenarios—are we about to enter a new era of LN photonics? June 24th, 2022

Advances in lithium niobate photonics: High performance integrated LN-based photonic devices have developed rapidly in recent years, and many different structures have been demonstrated for various application scenarios—are we about to enter a new era of LN photonics? June 24th, 2022

Boron nitride nanotube fibers get real: Rice lab creates first heat-tolerant, stable fibers from wet-spinning process June 24th, 2022

Announcements

New technology helps reveal inner workings of human genome June 24th, 2022

Advances in lithium niobate photonics: High performance integrated LN-based photonic devices have developed rapidly in recent years, and many different structures have been demonstrated for various application scenarios—are we about to enter a new era of LN photonics? June 24th, 2022

Advances in lithium niobate photonics: High performance integrated LN-based photonic devices have developed rapidly in recent years, and many different structures have been demonstrated for various application scenarios—are we about to enter a new era of LN photonics? June 24th, 2022

Boron nitride nanotube fibers get real: Rice lab creates first heat-tolerant, stable fibers from wet-spinning process June 24th, 2022

Tools

New technology helps reveal inner workings of human genome June 24th, 2022

Snapshot measurement of single nanostructure’s circular dichroism March 25th, 2022

Eyebrow-raising: Researchers reveal why nanowires stick to each other February 11th, 2022

JEOL Introduces New Scanning Electron Microscope with “Simple SEM” Automation and Live Elemental and 3D Analysis January 14th, 2022

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project