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Home > Press > Enzyme Biosensor Used for Rapid Measurement of Drug

Abstract:
Iranian researchers produced a new type of enzyme biosensor to increase the speed of clinical diagnosis.

Enzyme Biosensor Used for Rapid Measurement of Drug

Tehran, Iran | Posted on December 22nd, 2014

Biosensors have been recently used in medical, biological and industrial aspects to increase the speed of diagnosis. According to Bahareh Salehi, the executor of the plan, an enzyme biosensor has been used in this research to selectively diagnose penicillin by using silver nanoparticles. In fact, the role of silver nanoparticles was the facilitation of electron transfer between the elements of biosensor, and as a result, increasing its accuracy and respond speed.

The main application of the results is in pharmaceutics industries in the production of antibiotics. The biosensor can also be used in medical and biochemical sciences for the production of laboratorial and diagnosis kits. The proposed biosensor is considered as an appropriate replacement for the commonly-used liquid chromatography method. The advantage of the new tool is the simplicity of the production method and application, quick respond, selectivity, repeatability and reasonable production cost.

The penicillinase enzyme has been used in the production of the sensor. This enzyme easily absorbs penicillin even in the presence of disrupting species and provides accurate and desirable responds.

The results showed that the average diameter size of the nanoparticles has been about 90 nm in this research. The effects of temperature changes on the respond speed of the biosensor were studied at the range of 15-85°C after the production of the sensor and its modification with the synthetic nanoparticles. Observations showed gradual increase in the sensor respond as temperature increased. The maximum value was observed at about 50°C.

Results of the research have been published in International Journal of Electrochemical Science, vol. 9, issue 11, 2014, pp. 6201-6212.

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