Nanotechnology Now

Our NanoNews Digest Sponsors



Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Artificial enzyme mimics the natural detoxification mechanism in liver cells: Molybdenum oxide particles can assume the function of the endogenous enzyme sulfite oxidase / Basis for new therapeutic application

photo: Tremel research group
Mode of action of molybdenum oxide nanoparticles: (a) treatment of sulfite oxidase deficient liver cells; (b) mitochondria are directly targeted, nanoparticles accumulate in proximity to the membrane; (c) sulfite is oxidized to cellular innocuous sulfate.
photo: Tremel research group

Mode of action of molybdenum oxide nanoparticles: (a) treatment of sulfite oxidase deficient liver cells; (b) mitochondria are directly targeted, nanoparticles accumulate in proximity to the membrane; (c) sulfite is oxidized to cellular innocuous sulfate.

Abstract:
Scientists at Johannes Gutenberg University Mainz (JGU) in Germany have discovered that molybdenum trioxide nanoparticles oxidize sulfite to sulfate in liver cells in analogy to the enzyme sulfite oxidase. The functionalized Molybdenum trioxide nanoparticles can cross the cellular membrane and accumulate at the mitochondria, where they can recover the activity of sulfite oxidase.

Artificial enzyme mimics the natural detoxification mechanism in liver cells: Molybdenum oxide particles can assume the function of the endogenous enzyme sulfite oxidase / Basis for new therapeutic application

Mainz, Germany | Posted on June 30th, 2014

Sulfite oxidase is a molybdenum containing enzyme located in the mitochondria of liver and kidney cells, which catalyzes the oxidation of sulfite to sulfate during the protein and lipid metabolism and therefore plays an important role in cellular detoxification processes. A lack of functional sulfite oxidase is a rare but fatal genetic disease causing neurological disorders, mental retardation, physical deformities as well as degradation of the brain, which finally leads to premature death. Various dietary or drug treatments for a sulfite oxidase deficiency have been tried with moderate success.

It was the fact that molybdenum oxide is incorporated in the enzymes active site that provided the inspiration for the approach now taken by the team of scientists working under the lead of Professor Wolfgang Tremel of the JGU Institute of Inorganic Chemistry and Analytical Chemistry as well as Dr. Dennis Strand and Professor Susanne Strand of the Department of Internal Medicine of the Mainz University Medical Center. The researchers hope that this study may lay the basis for a therapeutic application of molybdenum trioxide nanoparticles and therefore new possibilities to treat sulfite oxidase deficiency.

Lowered sulfite oxidase levels can cause health problems even for otherwise healthy persons. In addition, sulfites are used as preservatives in food, e.g., in red wine, grape juice, or pickles in a jar. People having low levels of the sulfite oxidase react with symptoms like fatigue, asthma, drop in blood sugar, or headache.

With their study the Mainz scientists enter scientifically uncharted territory, because so far there are just a few studies of enzymatically active nanoparticles. "It is indeed astonishing, that simple inorganic nanoparticles can mimic an enzymatic activity," said Ruben Ragg, first author of this study. In a previous work Professor Wolfgang Tremel and his team had shown that vanadium oxide nanowires contain an enzymatically induced antifouling activity that efficiently prevents ships from being infested by marine microorganisms. "It is a long-standing goal of chemistry to synthesize artificial enzymes that imitate the essential and general principles of natural enzymes," added Tremel. There is growing evidence that nanoparticles can act as enzyme mimics. Some nanomaterials were reported to exhibit enzyme-like activities, but the hallmark of enzyme chemistry would be to catalyze transformations in cells in the presence of other competing reactions. This is difficult to achieve, as it requires compatibility with other cellular reactions operating under similar conditions and rates. Therefore, artificial enzymes are not only useful for an understanding of the reaction mechanism of native enzymes but also for future applications as therapeutic agents.

At the same time, the use of molybdenum nanoparticles would have several benefits. "Molybdenum oxide particles are considerably cheaper and also more stable than genetically produced enzymes," added Dr. Filipe Natalio, cooperation partner from Martin Luther University of Halle-Wittenberg. Natalio is designing new materials that can mimic complex structures found in nature by bringing together a wide range expertise from material sciences to biology and chemistry. The next steps of the project will be to test if the enzyme activity of the nanoparticles can be retained in living organisms.

The research teams were supported by an interdisciplinary grant from the JGU Center for Natural Sciences and Medicine (NMFZ) and the Max Planck Graduate Center (MPGC).

####

For more information, please click here

Contacts:
Professor Wolfgang Tremel

49-613-139-25135

Mainz University Medical Center
Professor Dr. Susanne Strand
Department of Internal Medicine
D 55131 Mainz
Tel +49 6131 17-9782

Copyright © Johannes Gutenberg Universitaet Mainz

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

University of Minnesota engineers make sound loud enough to bend light on a computer chip: Device could improve wireless communications systems November 28th, 2014

Study details laser pulse effects on behavior of electrons November 28th, 2014

Single-atom gold catalysts may offer path to low-cost production of fuel and chemicals November 28th, 2014

Production of Anticancer Drug from Nanofibers in Iran November 28th, 2014

Synthetic Biology

New tool could help reshape the limits of synthetic biology: The 'telomerator' reshapes synthetic yeast chromosome into more flexible, realistic form, redefining what geneticists can build November 3rd, 2014

Tiny carbon nanotube pores make big impact October 29th, 2014

Smallest world record has 'endless possibilities' for bio-nanotechnology October 8th, 2014

Artificial Cells Act Like the Real Thing: Cell-like compartments produce proteins and communicate with one another, similar to natural biological systems August 18th, 2014

Nanomedicine

Production of Anticancer Drug from Nanofibers in Iran November 28th, 2014

Vegetable oil ingredient key to destroying gastric disease bacteria: In mice, therapeutic nanoparticles dampen H. pylori bacteria and inflammation that lead to ulcers and gastric cancer November 25th, 2014

Research reveals how our bodies keep unwelcome visitors out of cell nuclei November 24th, 2014

ASU, IBM move ultrafast, low-cost DNA sequencing technology a step closer to reality November 24th, 2014

Discoveries

University of Minnesota engineers make sound loud enough to bend light on a computer chip: Device could improve wireless communications systems November 28th, 2014

Study details laser pulse effects on behavior of electrons November 28th, 2014

Production of Anticancer Drug from Nanofibers in Iran November 28th, 2014

The mysterious 'action at a distance' between liquid containers November 26th, 2014

Announcements

University of Minnesota engineers make sound loud enough to bend light on a computer chip: Device could improve wireless communications systems November 28th, 2014

Study details laser pulse effects on behavior of electrons November 28th, 2014

Single-atom gold catalysts may offer path to low-cost production of fuel and chemicals November 28th, 2014

Production of Anticancer Drug from Nanofibers in Iran November 28th, 2014

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

University of Minnesota engineers make sound loud enough to bend light on a computer chip: Device could improve wireless communications systems November 28th, 2014

Study details laser pulse effects on behavior of electrons November 28th, 2014

Single-atom gold catalysts may offer path to low-cost production of fuel and chemicals November 28th, 2014

Production of Anticancer Drug from Nanofibers in Iran November 28th, 2014

Nanobiotechnology

Quantum mechanical calculations reveal the hidden states of enzyme active sites November 20th, 2014

Tokyo Institute of Technology research: Protein-engineered cages aid studies of cell functions November 19th, 2014

A novel method for identifying the body’s ‘noisiest’ networks November 19th, 2014

Implementation of DNA Chains in Designing Nanospin Pieces November 9th, 2014

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More












ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE