Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > MEMS nanoinjector for genetic modification of cells: Researchers from Nexus Spine LLC and Brigham Young University have developed new, high-tech device for transferring DNA into cells

This SEM (scanning electron microscope) image shows the nanoinjector next to a latex bead the same size as an egg cell. You can see the size of the nanoinjector and its lance compared to a cell.

Credit: Brian Jensen/BYU
This SEM (scanning electron microscope) image shows the nanoinjector next to a latex bead the same size as an egg cell. You can see the size of the nanoinjector and its lance compared to a cell.

Credit: Brian Jensen/BYU

Abstract:
The ability to transfer a gene or DNA sequence from one animal into the genome of another plays a critical role in a wide range of medical research—including cancer, Alzheimer's disease, and diabetes.

MEMS nanoinjector for genetic modification of cells: Researchers from Nexus Spine LLC and Brigham Young University have developed new, high-tech device for transferring DNA into cells

Washington, DC | Posted on May 13th, 2014

But the traditional method of transferring genetic material into a new cell, called "microinjection," has a serious downside. It involves using a small glass pipette to pump a solution containing DNA into the nucleus of an egg cell, but the extra fluid can cause the cell to swell and destroy it—resulting in a 25 to 40 percent cell death rate.

Now, thanks to the work of researchers Brigham Young University, there's a way to avoid cell death when introducing DNA into egg cells. In Review of Scientific Instruments, the team describes its microelectromechanical system (MEMS) nanoinjector, which was designed to inject DNA into mouse zygotes (single-cell embryos consisting of a fertilized egg).

"Essentially, we use electrical forces to attract and repel DNA—allowing injections to occur with a tiny, electrically conductive lance," explained Brian Jensen, associate professor in the Department of Mechanical Engineering at Brigham Young University. "DNA is attracted to the outside of the lance using positive voltage, and then the lance is inserted into a cell."

The MEMS nanoinjector's lance is incredibly small and no extra fluid is used with this technique, so cells undergo much less stress compared to the traditional microinjection process.

This ability to inject DNA into cells without causing cell death leads to "more efficient injections, which in turn reduces the cost to create a transgenic animal," according to Jensen.

One of the team's most significant findings is that it's possible to use the electrical forces to get DNA into the nucleus of the cell—without having to carefully aim the lance into the pronucleus (the cellular structure containing the cell's DNA). "This may enable future automation of the injections, without requiring manual injection," Jensen says.

It may also mean that injections can be performed in animals with cloudy or opaque embryos. "Such animals, including many interesting larger ones like pigs, would be attractive for a variety of transgenic technologies," said Jensen. "We believe nanoinjection may open new fields of discovery in these animals."

As a next step, Jensen and colleagues are performing injections into cells in a cell culture using an array of lances that can inject hundreds of thousands of cells at once. "We expect the lance array may enable gene therapy using a culture of a patient's own cells," he noted.

####

About American Institute of Physics
The journal Review of Scientific Instruments, which is produced by AIP Publishing, presents innovation in instrumentation and methods across disciplines.

For more information, please click here

Contacts:
Jason Socrates Bardi

240-535-4954

Copyright © American Institute of Physics

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

The article "A Self-Reconfiguring Metamorphic Nanoinjector for Injection into Mouse Zygotes" by Quentin T. Aten, Brian D. Jensen, Sandra H. Burnett, and Larry L. Howell will be published in the journal Review of Scientific Instruments on Tuesday, May 13, 2014 (DOI: 10.1063/1.4872077). After that date, it will be available at:

Related News Press

News and information

Nano Ruffles in Brain Matter: Freiburg researchers decipher the role of nanostructures around brain cells in central nervous system function October 31st, 2014

Gold nanoparticle chains confine light to the nanoscale October 31st, 2014

'Nanomotor lithography' answers call for affordable, simpler device manufacturing October 31st, 2014

Device invented at Johns Hopkins provides up-close look at cancer on the move: Microscopic view of metastasis could give insight about how to keep cancer in check October 31st, 2014

Nanomedicine

Nano Ruffles in Brain Matter: Freiburg researchers decipher the role of nanostructures around brain cells in central nervous system function October 31st, 2014

Production of Biocompatible Polymers in Iran October 30th, 2014

Amorphous Coordination Polymer Particles as alternative to classical nanoplatforms for nanomedicine October 30th, 2014

'Electronic skin' could improve early breast cancer detection October 29th, 2014

Discoveries

Nano Ruffles in Brain Matter: Freiburg researchers decipher the role of nanostructures around brain cells in central nervous system function October 31st, 2014

Gold nanoparticle chains confine light to the nanoscale October 31st, 2014

'Nanomotor lithography' answers call for affordable, simpler device manufacturing October 31st, 2014

Device invented at Johns Hopkins provides up-close look at cancer on the move: Microscopic view of metastasis could give insight about how to keep cancer in check October 31st, 2014

Announcements

Nano Ruffles in Brain Matter: Freiburg researchers decipher the role of nanostructures around brain cells in central nervous system function October 31st, 2014

Gold nanoparticle chains confine light to the nanoscale October 31st, 2014

'Nanomotor lithography' answers call for affordable, simpler device manufacturing October 31st, 2014

Device invented at Johns Hopkins provides up-close look at cancer on the move: Microscopic view of metastasis could give insight about how to keep cancer in check October 31st, 2014

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals

Nano Ruffles in Brain Matter: Freiburg researchers decipher the role of nanostructures around brain cells in central nervous system function October 31st, 2014

'Nanomotor lithography' answers call for affordable, simpler device manufacturing October 31st, 2014

Device invented at Johns Hopkins provides up-close look at cancer on the move: Microscopic view of metastasis could give insight about how to keep cancer in check October 31st, 2014

Production of Biocompatible Polymers in Iran October 30th, 2014

Nanobiotechnology

Tiny carbon nanotube pores make big impact October 29th, 2014

Molecular beacons shine light on how cells 'crawl' October 27th, 2014

Breakthrough in molecular electronics paves the way for DNA-based computer circuits in the future: DNA-based programmable circuits could be more sophisticated, cheaper and simpler to make October 27th, 2014

NYU Researchers Break Nano Barrier to Engineer the First Protein Microfiber October 23rd, 2014

Alliances/Partnerships/Distributorships

'Electronic skin' could improve early breast cancer detection October 29th, 2014

European Commission opens the gate towards the implementation of Nanomedicine Translation Hub October 16th, 2014

IRLYNX and CEA-Leti to Streamline New CMOS-based Infrared Sensing Modules Dedicated to Human-activities Characterization October 15th, 2014

New VDMA Association "Electronics, Micro and Nano Technologies" founded: Inaugural Meeting in Frankfurt/Main, Germany October 15th, 2014

NanoNews-Digest
The latest news from around the world, FREE





  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE