Nanotechnology Now

Our NanoNews Digest Sponsors


Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > MEMS nanoinjector for genetic modification of cells: Researchers from Nexus Spine LLC and Brigham Young University have developed new, high-tech device for transferring DNA into cells

This SEM (scanning electron microscope) image shows the nanoinjector next to a latex bead the same size as an egg cell. You can see the size of the nanoinjector and its lance compared to a cell.

Credit: Brian Jensen/BYU
This SEM (scanning electron microscope) image shows the nanoinjector next to a latex bead the same size as an egg cell. You can see the size of the nanoinjector and its lance compared to a cell.

Credit: Brian Jensen/BYU

Abstract:
The ability to transfer a gene or DNA sequence from one animal into the genome of another plays a critical role in a wide range of medical research—including cancer, Alzheimer's disease, and diabetes.

MEMS nanoinjector for genetic modification of cells: Researchers from Nexus Spine LLC and Brigham Young University have developed new, high-tech device for transferring DNA into cells

Washington, DC | Posted on May 13th, 2014

But the traditional method of transferring genetic material into a new cell, called "microinjection," has a serious downside. It involves using a small glass pipette to pump a solution containing DNA into the nucleus of an egg cell, but the extra fluid can cause the cell to swell and destroy it—resulting in a 25 to 40 percent cell death rate.

Now, thanks to the work of researchers Brigham Young University, there's a way to avoid cell death when introducing DNA into egg cells. In Review of Scientific Instruments, the team describes its microelectromechanical system (MEMS) nanoinjector, which was designed to inject DNA into mouse zygotes (single-cell embryos consisting of a fertilized egg).

"Essentially, we use electrical forces to attract and repel DNA—allowing injections to occur with a tiny, electrically conductive lance," explained Brian Jensen, associate professor in the Department of Mechanical Engineering at Brigham Young University. "DNA is attracted to the outside of the lance using positive voltage, and then the lance is inserted into a cell."

The MEMS nanoinjector's lance is incredibly small and no extra fluid is used with this technique, so cells undergo much less stress compared to the traditional microinjection process.

This ability to inject DNA into cells without causing cell death leads to "more efficient injections, which in turn reduces the cost to create a transgenic animal," according to Jensen.

One of the team's most significant findings is that it's possible to use the electrical forces to get DNA into the nucleus of the cell—without having to carefully aim the lance into the pronucleus (the cellular structure containing the cell's DNA). "This may enable future automation of the injections, without requiring manual injection," Jensen says.

It may also mean that injections can be performed in animals with cloudy or opaque embryos. "Such animals, including many interesting larger ones like pigs, would be attractive for a variety of transgenic technologies," said Jensen. "We believe nanoinjection may open new fields of discovery in these animals."

As a next step, Jensen and colleagues are performing injections into cells in a cell culture using an array of lances that can inject hundreds of thousands of cells at once. "We expect the lance array may enable gene therapy using a culture of a patient's own cells," he noted.

####

About American Institute of Physics
The journal Review of Scientific Instruments, which is produced by AIP Publishing, presents innovation in instrumentation and methods across disciplines.

For more information, please click here

Contacts:
Jason Socrates Bardi

240-535-4954

Copyright © American Institute of Physics

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

The article "A Self-Reconfiguring Metamorphic Nanoinjector for Injection into Mouse Zygotes" by Quentin T. Aten, Brian D. Jensen, Sandra H. Burnett, and Larry L. Howell will be published in the journal Review of Scientific Instruments on Tuesday, May 13, 2014 (DOI: 10.1063/1.4872077). After that date, it will be available at:

Related News Press

News and information

Molybdenum disulfide holds promise for light absorption: Rice researchers probe light-capturing properties of atomically thin MoS2 May 5th, 2016

Oxford Instruments Asylum Research and McGill University Announce the McGill AFM Summer School and Workshop, May 12-13, 2016 May 4th, 2016

The intermediates in a chemical reaction photographed 'red-handed' Researchers at the UPV/EHU-University of the Basque Country have for the first time succeeded in imaging all the steps in a complex organic reaction and have resolved the mechanisms that explain it May 4th, 2016

New tool allows scientists to visualize 'nanoscale' processes May 4th, 2016

Nanomedicine

The intermediates in a chemical reaction photographed 'red-handed' Researchers at the UPV/EHU-University of the Basque Country have for the first time succeeded in imaging all the steps in a complex organic reaction and have resolved the mechanisms that explain it May 4th, 2016

Nuclear pores captured on film: Using an ultra fast-scanning atomic force microscope, researchers from the University of Basel have filmed 'living' nuclear pore complexes at work for the first time May 3rd, 2016

Little ANTs: Researchers build the world's tiniest engine May 3rd, 2016

Non-animal approach to predict impact of nanomaterials on human lung published Archives of Toxicology publishes workshop recommendations May 2nd, 2016

Discoveries

Molybdenum disulfide holds promise for light absorption: Rice researchers probe light-capturing properties of atomically thin MoS2 May 5th, 2016

The intermediates in a chemical reaction photographed 'red-handed' Researchers at the UPV/EHU-University of the Basque Country have for the first time succeeded in imaging all the steps in a complex organic reaction and have resolved the mechanisms that explain it May 4th, 2016

New tool allows scientists to visualize 'nanoscale' processes May 4th, 2016

Little ANTs: Researchers build the world's tiniest engine May 3rd, 2016

Announcements

Molybdenum disulfide holds promise for light absorption: Rice researchers probe light-capturing properties of atomically thin MoS2 May 5th, 2016

Oxford Instruments Asylum Research and McGill University Announce the McGill AFM Summer School and Workshop, May 12-13, 2016 May 4th, 2016

The intermediates in a chemical reaction photographed 'red-handed' Researchers at the UPV/EHU-University of the Basque Country have for the first time succeeded in imaging all the steps in a complex organic reaction and have resolved the mechanisms that explain it May 4th, 2016

New tool allows scientists to visualize 'nanoscale' processes May 4th, 2016

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

Molybdenum disulfide holds promise for light absorption: Rice researchers probe light-capturing properties of atomically thin MoS2 May 5th, 2016

The intermediates in a chemical reaction photographed 'red-handed' Researchers at the UPV/EHU-University of the Basque Country have for the first time succeeded in imaging all the steps in a complex organic reaction and have resolved the mechanisms that explain it May 4th, 2016

New tool allows scientists to visualize 'nanoscale' processes May 4th, 2016

An Experiment Seeks to Make Quantum Physics Visible to the Naked Eye May 3rd, 2016

Nanobiotechnology

The intermediates in a chemical reaction photographed 'red-handed' Researchers at the UPV/EHU-University of the Basque Country have for the first time succeeded in imaging all the steps in a complex organic reaction and have resolved the mechanisms that explain it May 4th, 2016

Nuclear pores captured on film: Using an ultra fast-scanning atomic force microscope, researchers from the University of Basel have filmed 'living' nuclear pore complexes at work for the first time May 3rd, 2016

Little ANTs: Researchers build the world's tiniest engine May 3rd, 2016

New drug-delivery approach holds potential for treating obesity May 2nd, 2016

Alliances/Trade associations/Partnerships/Distributorships

Electrically Conductive Graphene Ink Enables Printing of Biosensors April 23rd, 2016

Leti Extends Collaboration with Qualcomm on CoolCubeTM 3D Integration Technology for High-Density, High-Performance ICs: Collaboration Goals Include Building an Ecosystem To Take the Chip-stacking Technology from Design to Fabrication April 13th, 2016

FEI Partners with Five Pharmaceutical Companies, the Medical Research Council and the University of Cambridge to form Cryo-EM Research Consortium April 5th, 2016

Strem Chemicals and SONA Nanotech Sign Distribution Agreement for the World’s First Gold Nanorods Synthesized without CTAB February 24th, 2016

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







Car Brands
Buy website traffic