Nanotechnology Now







Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Berkeley Lab Researchers Demonstrate First Size-based Chromatography Technique for the Study of Living Cells

With size-based chromatography, a hexagonally ordered array of gold nanoparticles is fabricated onto a hybrid live cell-supported membrane. Membrane components move freely through the array provided they don’t exceed its physical dimensions. This reveals organizational aspects of the membrane environment unobservable by other techniques.
With size-based chromatography, a hexagonally ordered array of gold nanoparticles is fabricated onto a hybrid live cell-supported membrane. Membrane components move freely through the array provided they don’t exceed its physical dimensions. This reveals organizational aspects of the membrane environment unobservable by other techniques.

Abstract:
Using nanodot technology, Berkeley Lab researchers have demonstrated the first size-based form of chromatography that can be used to study the membranes of living cells. This unique physical approach to probing cellular membrane structures can reveal information critical to whether a cell lives or dies, remains normal or turns cancerous, that can't be obtained through conventional microscopy.

Berkeley Lab Researchers Demonstrate First Size-based Chromatography Technique for the Study of Living Cells

Berkeley, CA | Posted on April 22nd, 2014

"We've developed membrane-embedded nanodot array platforms that provide a physical means to both probe and manipulate membrane assemblies, including signaling clusters, while they are functioning in the membrane of a living cell," says Jay Groves, a chemist with Berkeley Lab's Physical Biosciences Division, who led this research.

Groves, who is also a professor with the University of California (UC) Berkeley's Chemistry Department, and a Howard Hughes Medical Institute (HHMI) investigator, is a recognized leader in developing techniques for studying the impact of spatial patterns on living cells. The live-cell supported synthetic membranes he and his group have been developing are constructed out of lipids and assembled onto a substrate of solid silica. These membranes are being used to determine how living cells not only interact with their environment through chemical signals but also through physical force and spatial patterns.

"We call our approach the spatial mutation strategy because molecules in a cell can be spatially re-arranged without altering the cell in any other way," Groves says. "Our live cell-supported membranes provide a hybrid interface consisting of mobile and immobile components with controlled geometry that allows us to utilize solid-state nanotechnology to manipulate and control molecular systems inside living cells."

While the work of Groves and others in recent years has demonstrated the importance of protein and lipid spatial organization within cellular membranes, details as to how spatial organization is tied to function are scarce primarily because of the limitations of optical microscopy at length scales below the 250 nanometer diffraction limit. The size-based chromatography technique developed by Groves and his group allows them to probe supramolecular structures in a cell membrane at the needed nanometer length-scales.

"We now have a way to translate nano-sized structures that approach molecular dimensions into geometric constraints on the movement of molecules inside a living cell," Groves says.

For their size-based chromatography technique, the spacing of proteins and other cellular molecules is controlled by a hexagonal or honeycomb array of gold nanoparticles that is fabricated into the membrane. The spacing between nanoparticles in each array can be controlled, with accessible sizes ranging from 30 to nearly 200 nanometers.

"Individual membrane components move freely throughout the array, but movement of larger assemblies is impeded if they exceed the physical dimensions of the array, Groves says.

Groves and his colleagues tested their size-based chromatography technique on T cell receptor (TCR) microclusters in T cell membranes, which is the functional module for antigen recognition by T cells (lymphocytes from the thymus) in the body's immune system. These TCR signaling clusters occupy a size regime ranging from tens to a few hundred nanometers, which is typically below the diffraction limit of conventional optical microscopy. Size-based chromatography was used to probe the physical properties of TCR signaling clusters as a function of antigen density. The results revealed that TCR signaling cluster is distinctly dependent on the amount of antigen encountered by the cell.

"This is something we did not know before about the TCR microcluster signaling system, which has been well-studied using conventional optical microscopy," Groves says. "It is a proof-of-principle demonstration that represents another step in the direction of interfacing living cells with synthetic materials to achieve molecular level control of the cell."

A paper on this research has been published in NANO Letters. The paper is titled "Size-based chromatography of signaling clusters in a living cell membrane." Groves is the corresponding author. Others authors are Niña Caculitan, Hiroyuki Kai, Eulanca Liu, Nicole Fay, Yan Yu, Theobald Lohmüller and Geoff O'Donoghue.

####

For more information, please click here

Contacts:
Lynn Yarris
(510) 486-5375

Copyright © DOE/Lawrence Berkeley National Laboratory

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

Download paper:

For more about the research of Jay Groves go here:

Related News Press

News and information

Antibacterial Ceramic Nanoparticles, Appropriate Material for Medical Devices May 3rd, 2015

Oxford Instruments announces winners of the 2015 Sir Martin Wood Science Prize for China May 2nd, 2015

Time Dependant Spectroscopy of Microscopic Samples: CRAIC TimePro™ software is used with CRAIC Technologies microspectrometers to measure the kinetic UV-visible-NIR, Raman and fluorescence spectra of microscopic sample areas May 2nd, 2015

ORNL researchers probe chemistry, topography and mechanics with one instrument May 2nd, 2015

Production of Industrial Nano-Membrane for Water, Wastewater Purification Device in Iran May 2nd, 2015

Imaging

Time Dependant Spectroscopy of Microscopic Samples: CRAIC TimePro™ software is used with CRAIC Technologies microspectrometers to measure the kinetic UV-visible-NIR, Raman and fluorescence spectra of microscopic sample areas May 2nd, 2015

ORNL researchers probe chemistry, topography and mechanics with one instrument May 2nd, 2015

Novel superconducting undulator provides first x-ray light at ANKA May 1st, 2015

Laboratories

ORNL researchers probe chemistry, topography and mechanics with one instrument May 2nd, 2015

Govt.-Legislation/Regulation/Funding/Policy

ORNL researchers probe chemistry, topography and mechanics with one instrument May 2nd, 2015

Making robots more human April 29th, 2015

Artificial photosynthesis could help make fuels, plastics and medicine April 29th, 2015

Research seeks alternatives for reducing bacteria in fresh produce using nanoengineering April 29th, 2015

Nanomedicine

Antibacterial Ceramic Nanoparticles, Appropriate Material for Medical Devices May 3rd, 2015

Polymeric Nanocarriers Improve Performance of Anticancer Drugs April 30th, 2015

Artificial photosynthesis could help make fuels, plastics and medicine April 29th, 2015

A phone with the ultimate macro feature: New attachment turns a smartphone into a microscope that can image and size DNA molecules 50,000 times thinner than a human hair April 29th, 2015

Discoveries

Antibacterial Ceramic Nanoparticles, Appropriate Material for Medical Devices May 3rd, 2015

ORNL researchers probe chemistry, topography and mechanics with one instrument May 2nd, 2015

Novel superconducting undulator provides first x-ray light at ANKA May 1st, 2015

Engineering a better solar cell: UW research pinpoints defects in popular perovskites May 1st, 2015

Announcements

Antibacterial Ceramic Nanoparticles, Appropriate Material for Medical Devices May 3rd, 2015

Nanometrics to Present at the B. Riley & Co. 16th Annual Investor Conference May 2nd, 2015

Time Dependant Spectroscopy of Microscopic Samples: CRAIC TimePro™ software is used with CRAIC Technologies microspectrometers to measure the kinetic UV-visible-NIR, Raman and fluorescence spectra of microscopic sample areas May 2nd, 2015

ORNL researchers probe chemistry, topography and mechanics with one instrument May 2nd, 2015

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

Antibacterial Ceramic Nanoparticles, Appropriate Material for Medical Devices May 3rd, 2015

ORNL researchers probe chemistry, topography and mechanics with one instrument May 2nd, 2015

FEI Company: Strong Growth Prospects Remain May 1st, 2015

Engineering a better solar cell: UW research pinpoints defects in popular perovskites May 1st, 2015

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project