Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > New Nanopore Sensor Simplifies Analysis of Methylated DNA

Abstract:
DNA methylation, the addition of a methyl group to specific locations on a DNA strand, plays a critical role in determining which genes are active in a cell at any given time. It plays an important role in embryonic development, cell growth and reproduction, and many diseases, including cancer. Now, researchers collaborating at the Mayo Clinic and the University of Illinois in Urbana-Champaign have developed a novel single molecule test for detecting DNA methylation that should greatly simplify and advance the study of this important genomic process.

New Nanopore Sensor Simplifies Analysis of Methylated DNA

Bethesda, MD | Posted on May 20th, 2013

The details of this new test appear in a paper published in the journal Scientific Reports. This study was led by George Vasmatzis, co-leader of the Mayo Clinic's Biomarker Discovery Program in the Center for Individualized Medicine, and Rashid Bashir, co-principal investigator of the Midwest Cancer Nanotechnology Training Center at the University of Illinois, part of the National Cancer Institute's Alliance for Nanotechnology in Cancer.

The new method relies on solid-state nanopores, nanometer-sized holes created using standard semiconductor processing technologies in membranes made of a particular type of insulating material known as a dielectric. Electrical signals from the dielectric change in specific patterns when molecules, such as DNA pass through the nanopore. In this case, the collaborating teams labeled methylated regions of DNA with a specific methyl DNA binding protein known as MBD1.Whenever the protein-labeled region of DNA passes through a nanopore, the electrical current changes by a factor of three compared to when unlabeled regions of DNA pass through the pore, an easily observed change.

"While nanopores have been studied for genomic sequencing and screening analysis, this new assay can potentially circumvent the need for some of the current processes in evaluating epigenetics-related diseases," says Dr. Vasmatzis. He says the assay could eliminate the need for bisulfite conversion of DNA, fluorescent labeling, and polymerase chain reaction (PCR), the standard method for detecting methylated DNA. While this method is useful, its limitation is that it requires large quantities of DNA.

In its current form, this new technique can detect single instances of DNA methylation with high fidelity and determine the total number of methylation sites per DNA molecule. According to Dr. Bashir, "The next step in this research is to increase the spatial resolution by incorporating thinner membranes and by integrating the same preparation steps." Such improvements would then enable researchers to create high-resolution methylation maps that would be useful for characterizing so-called epigenetic diseases, including cancer.

The investigators note that "cancer-specific methylated DNA from most tumor types are known to be present in biopsy specimens and in patient serum at very low concentrations. A rapid, accurate, and amplification free assay to detect these biomarkers from minute sample volumes could prove invaluable in the early detection of disease, monitoring disease progression, and prognosis. With continued development, solid-state nanopores could meet this unmet technological and clinical need."

####

About The National Cancer Institute (NCI)
To help meet the goal of reducing the burden of cancer, the National Cancer Institute (NCI), part of the National Institutes of Health, is engaged in efforts to harness the power of nanotechnology to radically change the way we diagnose, treat and prevent cancer.

The NCI Alliance for Nanotechnology in Cancer is a comprehensive, systematized initiative encompassing the public and private sectors, designed to accelerate the application of the best capabilities of nanotechnology to cancer.

Currently, scientists are limited in their ability to turn promising molecular discoveries into benefits for cancer patients. Nanotechnology can provide the technical power and tools that will enable those developing new diagnostics, therapeutics, and preventives to keep pace with today’s explosion in knowledge.

For more information, please click here

Contacts:
National Cancer Institute
Office of Technology & Industrial Relations
ATTN: NCI Alliance for Nanotechnology in Cancer
Building 31, Room 10A49
31 Center Drive , MSC 2580
Bethesda , MD 20892-2580

Copyright © The National Cancer Institute (NCI)

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

View full paper - "Detection and quantification of methylation in DNA using solid-state nanopores."

Related News Press

Govt.-Legislation/Regulation/Funding/Policy

Chiral magnetic effect generates quantum current: Separating left- and right-handed particles in a semi-metallic material produces anomalously high conductivity February 8th, 2016

Canadian physicists discover new properties of superconductivity February 8th, 2016

Leading bugs to the death chamber: A kinder face of cholesterol February 8th, 2016

From allergens to anodes: Pollen derived battery electrodes February 8th, 2016

Nanomedicine

Leading bugs to the death chamber: A kinder face of cholesterol February 8th, 2016

UTHealth research looks at nanotechnology to help prevent preterm birth February 7th, 2016

Scientists take key step toward custom-made nanoscale chemical factories: Berkeley Lab researchers part of team that creates new function in tiny protein shell structures February 6th, 2016

Hepatitis virus-like particles as potential cancer treatment February 5th, 2016

Sensors

Scientists have put a high precision blood assay into a simple test strip: Researchers have developed a new biosensor test system based on magnetic nanoparticles February 3rd, 2016

Nanosheet growth technique could revolutionize nanomaterial production February 1st, 2016

New record in nanoelectronics at ultralow temperatures January 28th, 2016

NBC LEARN DEBUTS SIX-PART VIDEO SERIES, “NANOTECHNOLOGY: SUPER SMALL SCIENCE” Produced by NBC Learn in partnership with the National Science Foundation, and narrated by NBC News/MSNBC’s Kate Snow, series highlights leading research in nanotechnology January 25th, 2016

Announcements

Scientists create laser-activated superconductor February 8th, 2016

Canadian physicists discover new properties of superconductivity February 8th, 2016

Leading bugs to the death chamber: A kinder face of cholesterol February 8th, 2016

From allergens to anodes: Pollen derived battery electrodes February 8th, 2016

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

Metal oxide sandwiches: New option to manipulate properties of interfaces February 8th, 2016

Canadian physicists discover new properties of superconductivity February 8th, 2016

Leading bugs to the death chamber: A kinder face of cholesterol February 8th, 2016

From allergens to anodes: Pollen derived battery electrodes February 8th, 2016

Energy

Canadian physicists discover new properties of superconductivity February 8th, 2016

Host-guest nanowires for efficient water splitting and solar energy storage February 7th, 2016

February 4th, 2016

Putting silicon 'sawdust' in a graphene cage boosts battery performance: Approach could remove major obstacles to increasing the capacity of lithium-ion batteries January 30th, 2016

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







Car Brands
Buy website traffic