Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > New Nanopore Sensor Simplifies Analysis of Methylated DNA

Abstract:
DNA methylation, the addition of a methyl group to specific locations on a DNA strand, plays a critical role in determining which genes are active in a cell at any given time. It plays an important role in embryonic development, cell growth and reproduction, and many diseases, including cancer. Now, researchers collaborating at the Mayo Clinic and the University of Illinois in Urbana-Champaign have developed a novel single molecule test for detecting DNA methylation that should greatly simplify and advance the study of this important genomic process.

New Nanopore Sensor Simplifies Analysis of Methylated DNA

Bethesda, MD | Posted on May 20th, 2013

The details of this new test appear in a paper published in the journal Scientific Reports. This study was led by George Vasmatzis, co-leader of the Mayo Clinic's Biomarker Discovery Program in the Center for Individualized Medicine, and Rashid Bashir, co-principal investigator of the Midwest Cancer Nanotechnology Training Center at the University of Illinois, part of the National Cancer Institute's Alliance for Nanotechnology in Cancer.

The new method relies on solid-state nanopores, nanometer-sized holes created using standard semiconductor processing technologies in membranes made of a particular type of insulating material known as a dielectric. Electrical signals from the dielectric change in specific patterns when molecules, such as DNA pass through the nanopore. In this case, the collaborating teams labeled methylated regions of DNA with a specific methyl DNA binding protein known as MBD1.Whenever the protein-labeled region of DNA passes through a nanopore, the electrical current changes by a factor of three compared to when unlabeled regions of DNA pass through the pore, an easily observed change.

"While nanopores have been studied for genomic sequencing and screening analysis, this new assay can potentially circumvent the need for some of the current processes in evaluating epigenetics-related diseases," says Dr. Vasmatzis. He says the assay could eliminate the need for bisulfite conversion of DNA, fluorescent labeling, and polymerase chain reaction (PCR), the standard method for detecting methylated DNA. While this method is useful, its limitation is that it requires large quantities of DNA.

In its current form, this new technique can detect single instances of DNA methylation with high fidelity and determine the total number of methylation sites per DNA molecule. According to Dr. Bashir, "The next step in this research is to increase the spatial resolution by incorporating thinner membranes and by integrating the same preparation steps." Such improvements would then enable researchers to create high-resolution methylation maps that would be useful for characterizing so-called epigenetic diseases, including cancer.

The investigators note that "cancer-specific methylated DNA from most tumor types are known to be present in biopsy specimens and in patient serum at very low concentrations. A rapid, accurate, and amplification free assay to detect these biomarkers from minute sample volumes could prove invaluable in the early detection of disease, monitoring disease progression, and prognosis. With continued development, solid-state nanopores could meet this unmet technological and clinical need."

####

About The National Cancer Institute (NCI)
To help meet the goal of reducing the burden of cancer, the National Cancer Institute (NCI), part of the National Institutes of Health, is engaged in efforts to harness the power of nanotechnology to radically change the way we diagnose, treat and prevent cancer.

The NCI Alliance for Nanotechnology in Cancer is a comprehensive, systematized initiative encompassing the public and private sectors, designed to accelerate the application of the best capabilities of nanotechnology to cancer.

Currently, scientists are limited in their ability to turn promising molecular discoveries into benefits for cancer patients. Nanotechnology can provide the technical power and tools that will enable those developing new diagnostics, therapeutics, and preventives to keep pace with today’s explosion in knowledge.

For more information, please click here

Contacts:
National Cancer Institute
Office of Technology & Industrial Relations
ATTN: NCI Alliance for Nanotechnology in Cancer
Building 31, Room 10A49
31 Center Drive , MSC 2580
Bethesda , MD 20892-2580

Copyright © The National Cancer Institute (NCI)

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

View full paper - "Detection and quantification of methylation in DNA using solid-state nanopores."

Related News Press

Govt.-Legislation/Regulation/Funding/Policy

Novel Rocket Design Flight Tested: New Rocket Propellant and Motor Design Offers High Performance and Safety October 23rd, 2014

Strengthening thin-film bonds with ultrafast data collection October 23rd, 2014

Brookhaven Lab Launches Computational Science Initiative:Leveraging computational science expertise and investments across the Laboratory to tackle "big data" challenges October 22nd, 2014

Bipolar Disorder Discovery at the Nano Level: Tiny structures found in brain synapses help scientists better understand disorder October 22nd, 2014

Nanomedicine

NYU Researchers Break Nano Barrier to Engineer the First Protein Microfiber October 23rd, 2014

Iranian Scientists Apply Nanotechnology to Produce Surgery Suture October 23rd, 2014

RF Heating of Magnetic Nanoparticles Improves the Thawing of Cryopreserved Biomaterials October 23rd, 2014

Sopping up proteins with thermosponges: Researchers develop novel nanoparticle platform that proves effective in delivering protein-based drugs October 22nd, 2014

Sensors

MEMS & Sensors Technology Showcase: Finalists Announced for MEMS Executive Congress US 2014 October 23rd, 2014

Journal Nanotechnology Progress International (JONPI), 2014, Volume 5, Issue 1, pp 1-24 October 22nd, 2014

Imaging electric charge propagating along microbial nanowires October 20th, 2014

Graphenea opens US branch October 16th, 2014

Announcements

Haydale Secures Exclusive Development and Supply Agreement with Tantec A/S: New reactors to be built and commissioned by Tantec A/S represent another step forward towards the commercialisation of graphene October 24th, 2014

QuantumWise guides the semiconductor industry towards the atomic scale October 24th, 2014

Advancing thin film research with nanostructured AZO: Innovnano’s unique and cost-effective AZO sputtering targets for the production of transparent conducting oxides October 23rd, 2014

Strengthening thin-film bonds with ultrafast data collection October 23rd, 2014

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals

NYU Researchers Break Nano Barrier to Engineer the First Protein Microfiber October 23rd, 2014

Iranian Scientists Apply Nanotechnology to Produce Surgery Suture October 23rd, 2014

Iranian, Malaysian Scientists Study Nanophotocatalysts for Water Purification October 23rd, 2014

Strengthening thin-film bonds with ultrafast data collection October 23rd, 2014

Energy

Nanoparticle technology triples the production of biogas October 23rd, 2014

Advancing thin film research with nanostructured AZO: Innovnano’s unique and cost-effective AZO sputtering targets for the production of transparent conducting oxides October 23rd, 2014

Researchers patent a nanofluid that improves heat conductivity October 22nd, 2014

Could I squeeze by you? Ames Laboratory scientists model molecular movement within narrow channels of mesoporous nanoparticles October 21st, 2014

NanoNews-Digest
The latest news from around the world, FREE





  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE