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Iranian researchers at the University of Kurdistan found a new method based on a nanocomposite comprised of carbon nanotubes to measure trace levels of a special type of human immune system's antibody.
Immunoglobulin E (IgE) is an antibody found in very low levels in human blood and is secreted in response to immediate reactions triggered by the hypersensitivities caused by allergic diseases such as asthma and hay fever. As a result, sensors detecting extremely low levels of IgE would be of interest and application in clinical diagnosis of patients suffering from special types of allergies. So far, researchers from all around the globe have been continually making efforts to develop such aptasensors for determination of Immunoglobulin E in human serum with desired precision.
According to the researchers, the synthesized nanocomposite provides an appropriate substrate for attachment and stabilization of enzymes and proteins. The good agreement between the obtained results and those by the ELISA standard confirm the high efficiency of the devised aptasensor.
The winning features of the proposed nanocomposite include the ease of synthesis and modification of the electrodes surface, the low cost of the modified electrodes and their high reproducibility, satisfactory performance of the nanobio-sensor for real specimens, low detection range and a broad linear operation range.
An elaborate report on this scientific research has been recently published in Biosensors and Bioelectronics, Vol. 43, 2013, pages 218 to 225.
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