Nanotechnology Now





Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > New imaging technology could reveal cellular secrets

This image illustrates the concept for a new type of technology that combines two biological imaging methods - atomic force microscopy and nuclear magnetic resonance - to create a new way to study cancer-cell metastasis and other disease-related processes.Purdue University image/ Xin Xu
This image illustrates the concept for a new type of technology that combines two biological imaging methods - atomic force microscopy and nuclear magnetic resonance - to create a new way to study cancer-cell metastasis and other disease-related processes.

Purdue University image/ Xin Xu

Abstract:
Atomic Force Microscopy-Coupled Microcoils for Cellular-Scale Nuclear Magnetic Resonance Spectroscopy

Charilaos Mousoulis,1 Teimour Maleki,2 Babak Ziaie,1,2,3 and Corey P. Neu1,a

1 1Weldon School of Biomedical Engineering, Purdue University

2 2Department of Electrical and Computer Engineering, Purdue University

3 Birck Nanotechnology Center, Purdue University

We present the coupling of atomic force microscopy (AFM) and nuclear magnetic resonance (NMR) technologies to enable topographical, mechanical and chemical profiling of biological samples. Here, we fabricate and perform proof-of-concept testing of radiofrequency planar microcoils on commercial AFM cantilevers. The sensitive region of the coil was estimated to cover an approximate volume of 19.4 10 3 lm3 (19.4 pl). Functionality of the spectroscopic module of the prototype device is illustrated through the detection of 1H resonance in deionized water. The acquired spectra 14 depict combined NMR capability with AFM that may ultimately enable biophysical and biochemical studies at the single cell level. VC 2013 AIP Publishing LLC. [dx.doi.org/10.1063/1.4801318].

New imaging technology could reveal cellular secrets

West Lafayette, IN | Posted on April 25th, 2013

Researchers have married two biological imaging technologies, creating a new way to learn how good cells go bad.

"Let's say you have a large population of cells," said Corey Neu, an assistant professor in Purdue University's Weldon School of Biomedical Engineering. "Just one of them might metastasize or proliferate, forming a cancerous tumor. We need to understand what it is that gives rise to that one bad cell."

Such an advance makes it possible to simultaneously study the mechanical and biochemical behavior of cells, which could provide new insights into disease processes, said biomedical engineering postdoctoral fellow Charilaos "Harris" Mousoulis.

Being able to study a cell's internal workings in fine detail would likely yield insights into the physical and biochemical responses to its environment. The technology, which combines an atomic force microscope and nuclear magnetic resonance system, could help researchers study individual cancer cells, for example, to uncover mechanisms leading up to cancer metastasis for research and diagnostics.

The prototype's capabilities were demonstrated by taking nuclear magnetic resonance spectra of hydrogen atoms in water. Findings represent a proof of concept of the technology and are detailed in a research paper that appeared online April 11 in the journal Applied Physics Letters. The paper was co-authored by Mousoulis; research scientist Teimour Maleki; Babak Ziaie, a professor of electrical and computer engineering; and Neu.

"You could detect many different types of chemical elements, but in this case hydrogen is nice to detect because it's abundant," Neu said. "You could detect carbon, nitrogen and other elements to get more detailed information about specific biochemistry inside a cell."

An atomic force microscope (AFM) uses a tiny vibrating probe called a cantilever to yield information about materials and surfaces on the scale of nanometers, or billionths of a meter. Because the instrument enables scientists to "see" objects far smaller than possible using light microscopes, it could be ideal for studying molecules, cell membranes and other biological structures.

However, the AFM does not provide information about the biological and chemical properties of cells. So the researchers fabricated a metal microcoil on the AFM cantilever. An electrical current is passed though the coil, causing it to exchange electromagnetic radiation with protons in molecules within the cell and inducing another current in the coil, which is detected.

The Purdue researchers perform "mechanobiology" studies to learn how forces exerted on cells influence their behavior. In work focusing on osteoarthritis, their research includes the study of cartilage cells from the knee to learn how they interact with the complex matrix of structures and biochemistry between cells.

Future research might include studying cells in "microfluidic chambers" to test how they respond to specific drugs and environmental changes.

A U.S. patent application has been filed for the concept. The research has been funded by Purdue's Showalter Trust Fund and the National Institutes of Health.

####

For more information, please click here

Contacts:
Writer:
Emil Venere
765-494-4709


Sources:
Corey Neu
765-496-1426


Charilaos "Harris" Mousoulis

Copyright © Purdue University

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Stanford breakthrough heralds super-efficient light-based computers: Light can transmit more data while consuming far less power than electricity, and an engineering feat brings optical data transport closer to replacing wires May 29th, 2015

Donuts, math, and superdense teleportation of quantum information May 29th, 2015

OSU researchers prove magnetism can control heat, sound: Team leverages OSC services to help confirm, interpret experimental findings May 29th, 2015

Two UCSB Professors Receive Early Career Research Awards: The Department of Energy’s award for young scientists acknowledges UC Santa Barbara’s standing as a top tier research institution May 29th, 2015

Global Carbon Nanotubes (CNT) Market Expected To Reach USD 3.42 Billion By 2022 May 29th, 2015

Imaging

Two UCSB Professors Receive Early Career Research Awards: The Department of Energy’s award for young scientists acknowledges UC Santa Barbara’s standing as a top tier research institution May 29th, 2015

Nano-capsules designed for diagnosing malignant tumours: Japanese researchers have developed adaptable nano-capsules that can help in the diagnosis of glioblastoma cells - a highly invasive form of brain tumours May 28th, 2015

New technique speeds nanoMRI imaging: Multiplexing technique for nanoscale magnetic resonance imaging developed by researchers in Switzerland cuts normal scan time from two weeks to two days May 28th, 2015

Nanomedicine

New chip makes testing for antibiotic-resistant bacteria faster, easier: Researchers at the University of Toronto design diagnostic chip to reduce testing time from days to one hour, allowing doctors to pick the right antibiotic the first time May 28th, 2015

Arrowhead to Present at Jefferies 2015 Healthcare Conference May 27th, 2015

Seeing the action: UCSB researchers develop a novel device to image the minute forces and actions involved in cell membrane hemifusion May 27th, 2015

Nanotechnology identifies brain tumor types through MRI 'virtual biopsy' in animal studies: If results are confirmed in humans, tumor cells could someday be diagnosed by MRI imaging and treated with tumor-specific IV injections; new NIH grant will fund future study May 27th, 2015

Discoveries

Stanford breakthrough heralds super-efficient light-based computers: Light can transmit more data while consuming far less power than electricity, and an engineering feat brings optical data transport closer to replacing wires May 29th, 2015

Donuts, math, and superdense teleportation of quantum information May 29th, 2015

OSU researchers prove magnetism can control heat, sound: Team leverages OSC services to help confirm, interpret experimental findings May 29th, 2015

New technique speeds nanoMRI imaging: Multiplexing technique for nanoscale magnetic resonance imaging developed by researchers in Switzerland cuts normal scan time from two weeks to two days May 28th, 2015

Announcements

Stanford breakthrough heralds super-efficient light-based computers: Light can transmit more data while consuming far less power than electricity, and an engineering feat brings optical data transport closer to replacing wires May 29th, 2015

Donuts, math, and superdense teleportation of quantum information May 29th, 2015

OSU researchers prove magnetism can control heat, sound: Team leverages OSC services to help confirm, interpret experimental findings May 29th, 2015

Two UCSB Professors Receive Early Career Research Awards: The Department of Energy’s award for young scientists acknowledges UC Santa Barbara’s standing as a top tier research institution May 29th, 2015

Interviews/Book Reviews/Essays/Reports/Podcasts/Journals/White papers

Stanford breakthrough heralds super-efficient light-based computers: Light can transmit more data while consuming far less power than electricity, and an engineering feat brings optical data transport closer to replacing wires May 29th, 2015

Donuts, math, and superdense teleportation of quantum information May 29th, 2015

OSU researchers prove magnetism can control heat, sound: Team leverages OSC services to help confirm, interpret experimental findings May 29th, 2015

Global Carbon Nanotubes (CNT) Market Expected To Reach USD 3.42 Billion By 2022 May 29th, 2015

Tools

Two UCSB Professors Receive Early Career Research Awards: The Department of Energy’s award for young scientists acknowledges UC Santa Barbara’s standing as a top tier research institution May 29th, 2015

Seeing the action: UCSB researchers develop a novel device to image the minute forces and actions involved in cell membrane hemifusion May 27th, 2015

Physicists solve quantum tunneling mystery: ANU media release: An international team of scientists studying ultrafast physics have solved a mystery of quantum mechanics, and found that quantum tunneling is an instantaneous process May 27th, 2015

This Slinky lookalike 'hyperlens' helps us see tiny objects: The photonics advancement could improve early cancer detection, nanoelectronics manufacturing and scientists' ability to observe single molecules May 23rd, 2015

Patents/IP/Tech Transfer/Licensing

SouthWest NanoTechnologies Introduces AgeNT™ Transparent Conductor System at SID Display Week, Booth #543 May 28th, 2015

Researchers develop new way to manufacture nanofibers May 21st, 2015

Novel superconducting undulator provides first x-ray light at ANKA May 1st, 2015

Long Island Capital Alliance Announces Participants for Brookhaven National Laboratory Technology Transfer Capital Forum on May 8: Keynote Speaker Dr. Doon Gibbs, Director of Brookhaven National Laboratory April 16th, 2015

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project