Nanotechnology Now |
Home > Press > Engineering a new single-walled carbon nanotubes- molecular beacon-daunorubicin sensor for detection of Salmonella typhimurium carrying SSeC gene
Abstract:
A novel single-walled carbon nanotubes(SWNTs)-molecular beacons(MBs) -daunoru -bicin(DNR) conjugate was devised as a targeted sensing system to detect S. typhimurium. The system, signified as SWNTs-MB(DNR), was fabricated by covalently coupling of a MB included complementary sequence targeted to the SSeC of the bacteria through EDC/Sulfo-NHS chemistry and insetting daunorubicin to the stem region of the molecular beacon on carbon nanotubes before adding the target sequence.
Engineering a new single-walled carbon nanotubes- molecular beacon-daunorubicin sensor for detection of Salmonella typhimurium carrying SSeC gene
PR China | Posted on June 22nd, 2012The fluorescence of daunorubicin, originally, was fairly week because of dual fluorescence quenching. When mixing target sequence with SWNTs-MBs(DNR), it was seen that the daunorubicin was discharged from hairpin stem due to the formation of rigid structure between the loop of the MB and the target sequence, which resulted in increase of fluorescence intensity substantially and attained to target quantitation. On the contrary, mismatch oligonucleotides of other Salmonella sps. with different sero types did not enhance the fluorescence intensity considerably, which represented a high selectivity and specificity of the biosensor. The experimental results demonstrated patently that the recovery of fluorescence of daunorubicin is proportion to the concentration of the target DNA with the range 0.2-0.7ěM and the low detection limit is 50nM. Consequently, the SWNTs-MBs(DNR) architecture can be an excellent platform to take place of the common molecular beacon easily and has the potential to study MB based on aptamer binding to the SWNTs.
####
For more information, please click here
Contacts:
Le Deng
Phone: +86 0731 88872927
Fax: +86 0731 88883310
Copyright © The Co-construction Laboratory of Microb
If you have a comment, please Contact us.Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.
Bookmark:
| Related News Press |
News and information
Sound waves precisely position nanowires June 19th, 2013
Scientists Use Nanotechnology to Increase Thermal Stability of Essential Oils June 19th, 2013
Production of Bioactive Material for Quick Treatment of Bone Damages June 19th, 2013
Nanotubes/Buckyballs
Unzipped nanotubes unlock potential for batteries: Rice University lab combines graphene nanoribbons with tin oxide for improved anodes June 13th, 2013
The Diabetes ‘Breathalyzer’: Pitt chemists demonstrate sensor technology that could detect and monitor diabetes through breath analysis alone June 10th, 2013
Even with Defects, Graphene is Strongest Material in the World: New Study Reveals Strength of CVD Graphene May 31st, 2013
Nanomedicine
Production of Bioactive Material for Quick Treatment of Bone Damages June 19th, 2013
3-D printing could lead to tiny medical implants, electronics, robots, more June 18th, 2013
Pioneering breakthrough of chemical nanoengineering to design drugs controlled by light June 18th, 2013
Study Shows How the Nanog Protein Promotes Growth of Head and Neck Cancer June 18th, 2013
Sensors
Sound waves precisely position nanowires June 19th, 2013
Production of Polyaniline Biosensors Modified with Conductive Polymer Composites June 18th, 2013
Monell-led research identifies scent of melanoma: New research may lead to early non-invasive detection and diagnosis June 14th, 2013
Discoveries
Sound waves precisely position nanowires June 19th, 2013
Scientists Use Nanotechnology to Increase Thermal Stability of Essential Oils June 19th, 2013
Production of Bioactive Material for Quick Treatment of Bone Damages June 19th, 2013
New Method to Synthesize Zinc Oxide Nanoparticles with High Catalytic Activity June 18th, 2013
Announcements
Sound waves precisely position nanowires June 19th, 2013
Scientists Use Nanotechnology to Increase Thermal Stability of Essential Oils June 19th, 2013
Production of Bioactive Material for Quick Treatment of Bone Damages June 19th, 2013
Patents/IP/Tech Transfer/Licensing
Nanoparticle Opens the Door to Clean-Energy Alternatives June 14th, 2013
DRYWIRED™ Receives MILITARY-STANDARD Certifications from QUANTA LABORATORIES for Its Latest Protective Nano-Coating Technology June 5th, 2013
Apple patents point to slimmer battery tech June 1st, 2013
Patent for Coaxial Nanofibre Production: Scientists from Contipro have patented new jet for effective coaxial nanofibers production May 31st, 2013
Food/Agriculture/Supplements
Scientists Use Nanotechnology to Increase Thermal Stability of Essential Oils June 19th, 2013
Can nanotech save dying shrimp farms? June 15th, 2013
Lorraine University uses Nanoparticle Tracking Analysis to characterize biomolecules for agrichemicals, pharmacology and cosmetics May 28th, 2013
Biodegradable Nanocomposites Synthesized for Food Stuff Packaging May 27th, 2013
 |
||
 |
||
| The latest news from around the world, FREE | ||
 |
||
|
|
||
| Premium Products | ||
 |
||
|
Only the news you want to read!
Learn More |
||
 |
||
|
University Technology Transfer & Patents
Learn More |
||
 |
||
|
Full-service, expert consulting
Learn More |
||
|
|
||
abbigliamento uomo
Computer Accessories
| © Copyright 1999-2013 7th Wave, Inc. All Rights Reserved | PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE |