Nanotechnology Now

Our NanoNews Digest Sponsors



Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Engineering a new single-walled carbon nanotubes- molecular beacon-daunorubicin sensor for detection of Salmonella typhimurium carrying SSeC gene

Abstract:
A novel single-walled carbon nanotubes(SWNTs)-molecular beacons(MBs) -daunoru -bicin(DNR) conjugate was devised as a targeted sensing system to detect S. typhimurium. The system, signified as SWNTs-MB(DNR), was fabricated by covalently coupling of a MB included complementary sequence targeted to the SSeC of the bacteria through EDC/Sulfo-NHS chemistry and insetting daunorubicin to the stem region of the molecular beacon on carbon nanotubes before adding the target sequence.

Engineering a new single-walled carbon nanotubes- molecular beacon-daunorubicin sensor for detection of Salmonella typhimurium carrying SSeC gene

PR China | Posted on June 22nd, 2012

The fluorescence of daunorubicin, originally, was fairly week because of dual fluorescence quenching. When mixing target sequence with SWNTs-MBs(DNR), it was seen that the daunorubicin was discharged from hairpin stem due to the formation of rigid structure between the loop of the MB and the target sequence, which resulted in increase of fluorescence intensity substantially and attained to target quantitation. On the contrary, mismatch oligonucleotides of other Salmonella sps. with different sero types did not enhance the fluorescence intensity considerably, which represented a high selectivity and specificity of the biosensor. The experimental results demonstrated patently that the recovery of fluorescence of daunorubicin is proportion to the concentration of the target DNA with the range 0.2-0.7ìM and the low detection limit is 50nM. Consequently, the SWNTs-MBs(DNR) architecture can be an excellent platform to take place of the common molecular beacon easily and has the potential to study MB based on aptamer binding to the SWNTs.

####

For more information, please click here

Contacts:
Le Deng
Phone: +86 0731 88872927
Fax: +86 0731 88883310

Copyright © The Co-construction Laboratory of Microb

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

New research project supports internationalisation in nano-research: Launch of new “Baltic Sea Network” November 22nd, 2014

3rd Iran-Proposed Nano Standard Approved by International Standard Organization November 22nd, 2014

NMTI announces breakthrough solutions for HAMR nanoantenna for next-generation ultra-high density magnetic storage November 21st, 2014

Canatu Launches CNB In-Mold Film for Transparent Touch on 3D Surfaces –in Cars, Household Appliances, Wearables, Portables November 20th, 2014

Nanotubes/Buckyballs

Tesla NanoCoatings Increasing Use of SouthWest NanoTechnologies Carbon Nanotubes (CNTs) for its Infrastructure Coatings and Paints: High Quality SMW™ Specialty Multi-wall Carbon Nanotubes Incorporated into Teslan®-brand coatings used by Transportation, Oil and Gas Companies November 19th, 2014

Graphene/nanotube hybrid benefits flexible solar cells: Rice University labs create novel electrode for dye-sensitized cells November 17th, 2014

SouthWest NanoTechnologies to Demonstrate 3D Capacitive Touch Sensor Featuring Transparent, Thermoformed Carbon Nanotube Ink at Printed Electronics USA 2014 (Booth J25) -- “Conductive and Semiconducting Single-Wall Carbon Nanotube Inks” will be Topic of Company Presentation November 10th, 2014

Neural Canals Produced in Iran for Recovery of Sciatica Nerve November 8th, 2014

Nanomedicine

New research project supports internationalisation in nano-research: Launch of new “Baltic Sea Network” November 22nd, 2014

Quantum mechanical calculations reveal the hidden states of enzyme active sites November 20th, 2014

Silver Nanoparticles Produced in Iran from Forest Plants Extract November 20th, 2014

Tokyo Institute of Technology research: Protein-engineered cages aid studies of cell functions November 19th, 2014

Sensors

Canatu Launches CNB In-Mold Film for Transparent Touch on 3D Surfaces –in Cars, Household Appliances, Wearables, Portables November 20th, 2014

UO-industry collaboration points to improved nanomaterials: University of Oregon microscope puts spotlight on the surface structure of quantum dots for designing new solar devices November 20th, 2014

Spiraling light, nanoparticles and insights into life’s structure November 19th, 2014

New materials for more powerful solar cells: Major breakthrough in solar energy November 11th, 2014

Discoveries

NMTI announces breakthrough solutions for HAMR nanoantenna for next-generation ultra-high density magnetic storage November 21st, 2014

UO-industry collaboration points to improved nanomaterials: University of Oregon microscope puts spotlight on the surface structure of quantum dots for designing new solar devices November 20th, 2014

Silver Nanoparticles Produced in Iran from Forest Plants Extract November 20th, 2014

Nano Sorbents Able to Remove Pollutions Caused by Oil Derivatives November 20th, 2014

Announcements

New research project supports internationalisation in nano-research: Launch of new “Baltic Sea Network” November 22nd, 2014

3rd Iran-Proposed Nano Standard Approved by International Standard Organization November 22nd, 2014

NMTI announces breakthrough solutions for HAMR nanoantenna for next-generation ultra-high density magnetic storage November 21st, 2014

Nano Sorbents Able to Remove Pollutions Caused by Oil Derivatives November 20th, 2014

Patents/IP/Tech Transfer/Licensing

Dicerna Announces License Agreement with Tekmira to Advance Dicerna’s PH1 Development Program November 17th, 2014

First genetic-based tool to detect circulating cancer cells in blood: NanoFlares light up individual cells if breast cancer biomarker is present November 17th, 2014

Ki-Bum Lee Patents Technology To Advance Stem Cell Therapeutics November 13th, 2014

'Direct writing' of diamond patterns from graphite a potential technological leap November 5th, 2014

Food/Agriculture/Supplements

Production of Biodegradable Nanocomposites of Wheat Gluten in Iran November 6th, 2014

New tool could help reshape the limits of synthetic biology: The 'telomerator' reshapes synthetic yeast chromosome into more flexible, realistic form, redefining what geneticists can build November 3rd, 2014

Nanoparticles Display Ability to Improve Efficiency of Filters October 28th, 2014

Smallest world record has 'endless possibilities' for bio-nanotechnology October 8th, 2014

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More












ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE