Nanotechnology Now

Our NanoNews Digest Sponsors





Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Testing for Enzyme Activity using Gold Nanoparticles

Abstract:
Can you see just by looking whether or not an active enzyme is present in a sample? This is exactly what Chinese scientists have done by using a new protocol that combines gold nanoparticles with an enzyme substrate to give a simple test for the activity of an enzyme, the results of which can be seen with the naked eye.

Testing for Enzyme Activity using Gold Nanoparticles

Germany | Posted on November 24th, 2011

Human telomerase is an enzyme that catalyzes the addition of protective repeat units of DNA known as telomeres onto the human chromosome. This enzyme is overexpressed in over 85% of tumors, so it can act as a clear biomarker for the presence of a tumor, and it is already used in this way for diagnosis. However, the main current detection method for human telomerase involves amplification of the DNA by polymerase chain reaction (PCR), which carries with it an inherent possibility to produce artifacts. Other methods that have been used are either unsuitable for high-throughput procedures, relatively insensitive, or require complicated equipment to perform and read-out.

Now, researchers from the Chinese Academy of Sciences at Changchun Institute of Applied Chemistry, China, led by Xiaogang Qu have developed a new, easy-to-perform method to detect activity of human telomerase rapidly and at low concentrations of the enzyme.

Their method uses gold nanoparticles and is based on the fact that the usual substrate for telomerase is an oligonucleotide which, when attached to the surface of a gold nanoparticle, will fold in such a way to prevent aggregation of the particles. Addition or presence of the telomerase enzyme causes the substrate to cleave from the nanoparticle, which alters the aggregation of the particles. This change in aggregation then causes a color change in the solution that is visible with the naked eye when the concentration of cells is high enough, or can be measured using a UV-Vis spectrophotometer down to a concentration of a single HeLa cell per microliter.

The color change means that no complicated equipment is required to read-out the test result, and the whole analysis can be completed in a very short amount of time, unlike methods that work via PCR. All of this, combined with the relatively low cost of the test, means that it could easily be used in a high-throughput situation to test many samples quickly. As well as diagnosis of cancers, the authors have also tested their method as a way to screen for telomerase inhibitors that could act as possible anticancer drugs, so this work should be of great interest to scientists working in drug development as well as to medics both in the field and the laboratory.

####

For more information, please click here

Copyright © Wiley-VCH Materials Science Journals

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

J. Wang et al., Small ; DOI: 10.1002/smll.201101938

Related News Press

News and information

Imec Reports Four Percent Growth for 2013 Fiscal Year End —Continues to Accelerate Innovation Through Global Collaborations and Technological Breakthroughs in Nanoelectronics— April 24th, 2014

Multicapacity Microreactor for Catalyst Characterisation April 24th, 2014

Making graphene work for real-world devices: Fundamental research in phonon scattering helps researchers design graphene materials for applications April 24th, 2014

Return on investment for kit and promotion materials April 24th, 2014

Chemistry

Thinnest feasible membrane produced April 17th, 2014

Targeting cancer with a triple threat: MIT chemists design nanoparticles that can deliver three cancer drugs at a time April 15th, 2014

Scientists Succeed in Simultaneous Determination of Acetaminophen, Codeine in Drug Samples April 9th, 2014

Good vibrations: Using light-heated water to deliver drugs - Researchers use near-infrared light to warm water-infused polymeric particles April 1st, 2014

Discoveries

Making graphene work for real-world devices: Fundamental research in phonon scattering helps researchers design graphene materials for applications April 24th, 2014

Return on investment for kit and promotion materials April 24th, 2014

Protecting olive oil from counterfeiters April 24th, 2014

Gold nanoparticles help target, quantify breast cancer gene segments in a living cell April 23rd, 2014

Announcements

Imec Reports Four Percent Growth for 2013 Fiscal Year End —Continues to Accelerate Innovation Through Global Collaborations and Technological Breakthroughs in Nanoelectronics— April 24th, 2014

Multicapacity Microreactor for Catalyst Characterisation April 24th, 2014

Making graphene work for real-world devices: Fundamental research in phonon scattering helps researchers design graphene materials for applications April 24th, 2014

Return on investment for kit and promotion materials April 24th, 2014

NanoNews-Digest
The latest news from around the world, FREE







  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE