Nanotechnology Now

Our NanoNews Digest Sponsors



Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > Researcher Uses Nanoparticles to Make DNA Analysis 1,000 Times Faster

Abstract:
A University of Arkansas researcher has patented a process that reduces the time it takes to perform DNA analysis from hours to minutes. This development could contribute to many areas of health care and law enforcement, including diagnosing and treating disease, developing and testing new vaccines and forensic identification.

Researcher Uses Nanoparticles to Make DNA Analysis 1,000 Times Faster

Fayetteville, AR | Posted on October 3rd, 2011

Donald K. Roper, associate professor of chemical engineering, explained that the ultimate goal of his research is to develop a credit-card-sized device to be used in a doctor's office or at a crime scene to quickly analyze samples of DNA. "That's the power of being able to do this on a really tiny scale," he said.

To analyze DNA, scientists must often make a tiny sample large enough to work with. To do this, they use a process called polymerase chain reaction, or PCR. Roper, who holds the Charles W. Oxford Endowed Professorship in Emerging Technologies, has invented a way to perform this reaction thousands of times faster than traditional methods.

Roper's process, which he developed while working at the University of Utah, uses gold nanoparticles to increase the efficiency of the chain reaction. During the reaction, strands of DNA are heated and cooled in cycles. When the samples are heated, the two strands of a DNA double helix come apart, and when the temperature is lowered, an enzyme called polymerase zips each strand to other, complementary strands, forming two new DNA helixes.

These copies are then heated and cooled again, doubling each time until the desired amount of DNA has been produced.

Roper's method reduces the time involved in these cycles from minutes to milliseconds, which means that a DNA sample could be analyzed within minutes rather than hours. By associating the DNA and enzyme with a gold nanoparticle and then exciting the nanoparticle with a light source or laser beam, Roper can target temperature changes to the area immediately around the DNA. This allows researchers to raise or lower the temperature more quickly. In addition, the process can be used to analyze the DNA during the reaction.

"We can use the laser light and the gold nanoparticles to do both the amplification and the analysis simultaneously," explained Roper. "The electromagnetic field around the nanoparticle is strong enough that it can sense whether or not the strand that we're interested in is there. The laser induces the field and then a detector assays the difference in the field."

Roper's research has implications for many scientific fields. "Genomics underscores everything of interest to biology: gene sequencing, disease diagnostics, pharmaceutical development and genetic analysis," he explained. "DNA is the basis of inheritance for the cell, and the degree of transcription of the DNA determines how a cell will function. This is a tool that examines these processes."

####

For more information, please click here

Contacts:
Donald Roper
associate professor
chemical engineering
College of Engineering
479-575-6691


Camilla Medders
director of communications
College of Engineering
479-575-5697

Copyright © Newswise

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

Nanomedicine

Nanoscale assembly line August 29th, 2014

Copper shines as flexible conductor August 29th, 2014

Novel 'butterfly' molecule could build new sensors, photoenergy conversion devices August 28th, 2014

PetLife Comments on CNN Story on Scorpion Venom Health Benefits August 27th, 2014

Discoveries

A new, tunable device for spintronics: An international team of scientists including physicist Jairo Sinova from the University of Mainz realises a tunable spin-charge converter made of GaAs August 29th, 2014

Nanoscale assembly line August 29th, 2014

Copper shines as flexible conductor August 29th, 2014

Novel 'butterfly' molecule could build new sensors, photoenergy conversion devices August 28th, 2014

Announcements

A new, tunable device for spintronics: An international team of scientists including physicist Jairo Sinova from the University of Mainz realises a tunable spin-charge converter made of GaAs August 29th, 2014

Nanoscale assembly line August 29th, 2014

New analytical technology reveals 'nanomechanical' surface traits August 29th, 2014

New Vice President Takes Helm at CNSE CMOST: Catherine Gilbert To Lead CNSE Children’s Museum of Science and Technology Through Expansion And Relocation August 29th, 2014

Patents/IP/Tech Transfer/Licensing

New analytical technology reveals 'nanomechanical' surface traits August 29th, 2014

AQUANOVA receives Technology Leadership Award 2014 FROST & SULLIVAN honors NovaSOL® Technology again August 12th, 2014

Blacktrace Holdings Ltd. to in-license PerkinElmer Technology August 8th, 2014

Silicene Labs Announces the Launch of Patent-Pending, 2D Materials Composite Index™ : The Initial 2D Materials Composite Index™ for Q2 2014 Is: 857.3; Founders Include World-Renowned Physicist and Seasoned Business and IP Professionals July 24th, 2014

NanoNews-Digest
The latest news from around the world, FREE



  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More














ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE