Nanotechnology Now





Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Just Add Water and... Treat Brain Cancer: Freeze-dried gene therapy system avoids virus, potential complications

Brain cancer cells produce a green fluorescent protein. DNA encoded to produce the protein was delivered to the cancer cells by new freeze-dried nanoparticles produced by Johns Hopkins biomedical engineers.
Stephany Tzeng
Brain cancer cells produce a green fluorescent protein. DNA encoded to produce the protein was delivered to the cancer cells by new freeze-dried nanoparticles produced by Johns Hopkins biomedical engineers.

Stephany Tzeng

Abstract:
Researchers at the Johns Hopkins University School of Medicine have developed a technique that delivers gene therapy into human brain cancer cells using nanoparticles that can be freeze-dried and stored for up to three months prior to use.

Just Add Water and... Treat Brain Cancer: Freeze-dried gene therapy system avoids virus, potential complications

Baltimore, MD | Posted on July 8th, 2011

The shelf-stable particles may obviate the need for virus-mediated gene therapy, which has been associated with safety concerns. The report appears in the August issue of Biomaterials.

"Most nonviral gene therapy methods have very low efficacy," says Jordan Green, Ph.D., an assistant professor of biomedical engineering at Johns Hopkins. "Nanoparticle-based gene therapy has the potential to be both safer and more effective than conventional chemical therapies for the treatment of cancer."

To develop the nanoparticle, Green's team started with store-bought small molecules and systematically mixed combinations together to generate chemical reactions that resulted in different polymers. They then mixed DNA that encodes a glowing protein with each different polymer to allow the DNA to bind to the polymers and form nanoparticles. Each different sample was added to human brain tumor cells and human brain tumor stem cells. After 48 hours, the team examined and counted how many cells glowed from having taken up the nanoparticles and made the glowing protein encoded by the introduced DNA.

The team rated success by counting how many cells survived and what percentage of those cells glowed.

Of the many combinations they tested, the researchers found that one particular formulation of so-called poly(beta-amino ester) nanoparticles did particularly well at getting into both glioblastoma and brain tumor stem cells. The researchers then freeze-dried these nanoparticles and stored them at different temperatures (freezer, refrigerator and room temperature) for different lengths of time (one, two and up to three months), and then retested their ability to get into cells. According to Green, after six months in storage, the effectiveness dropped by about half, but they found that up to three months of storage at room temperature there was virtually no change in effectiveness.

Furthermore, the team found that certain nanoparticles had a particular affinity for brain tumor cells over healthy brain cells.

"I could imagine particles based on this technology being used in conjunction with, and even instead of brain surgery," says Alfredo Quinones-Hinojosa, M.D., Ph.D., an associate professor of neurosurgery and oncology at Johns Hopkins. "I envision that one day, as we understand the etiology and progression of brain cancer, we will be able to use these nanoparticles even before doing surgery-how nice would that be? Imagine avoiding brain surgery altogether."

This study was funded by the Institute for NanoBioTechnology at The Johns Hopkins University, the Maryland Stem Cell Research Fund, National Institutes of Health, the Howard Hughes Medical Institute and the Robert Wood Johnson Foundation.

Authors on the paper are Stephany Tzeng, Hugo Guerrero-Cazares, Elliott Martinez, Joel Sunshine, Alfredo Quinones-Hinojosa and Jordan Green, all of Johns Hopkins.

####

For more information, please click here

Contacts:
Media Contacts:
Mary Spiro
410-516-4802


Vanessa McMains
410-502-9410


Audrey Huang
410-614-5105


Maryalice Yakutchik
443-287-2251

Copyright © Johns Hopkins University School of Medicine

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related Links

Institute for NanoBioTechnology

Department of Biomedical Engineering

Jordan Green

Alfredo Quinones-Hinojosa

Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins

Related News Press

News and information

Surfing a wake of light: Researchers observe and control light wakes for the first time July 6th, 2015

Tel Aviv/Tsinghua University project uses crowd computing to improve water filtration: The research, a product of the new TAU-Tsinghua XIN Center, was conducted by 150,000 volunteers at IBM's World Community Grid July 6th, 2015

Transition from 3 to 2 dimensions increases conduction, MIPT scientists discover July 6th, 2015

A Stretchy Mesh Heater for Sore Muscles July 6th, 2015

Govt.-Legislation/Regulation/Funding/Policy

Surfing a wake of light: Researchers observe and control light wakes for the first time July 6th, 2015

New technology using silver may hold key to electronics advances July 2nd, 2015

NIST Group Maps Distribution of Carbon Nanotubes in Composite Materials July 2nd, 2015

Ultra-stable JILA microscopy technique tracks tiny objects for hours July 1st, 2015

Nanomedicine

A Stretchy Mesh Heater for Sore Muscles July 6th, 2015

New Biosensor Produced in Iran to Detect Effective Drugs in Cancer Treatment July 4th, 2015

Groundbreaking research to help control liquids at micro and nano scales July 3rd, 2015

Iranian Scientists Find Simple, Economic Method to Synthesize Antibacterial Nanoparticles July 2nd, 2015

Discoveries

Fundamental observation of spin-controlled electrical conduction in metals: Ultrafast terahertz spectroscopy yields direct insight into the building block of modern magnetic memories July 6th, 2015

Surfing a wake of light: Researchers observe and control light wakes for the first time July 6th, 2015

Transition from 3 to 2 dimensions increases conduction, MIPT scientists discover July 6th, 2015

A Stretchy Mesh Heater for Sore Muscles July 6th, 2015

Announcements

Surfing a wake of light: Researchers observe and control light wakes for the first time July 6th, 2015

Tel Aviv/Tsinghua University project uses crowd computing to improve water filtration: The research, a product of the new TAU-Tsinghua XIN Center, was conducted by 150,000 volunteers at IBM's World Community Grid July 6th, 2015

Transition from 3 to 2 dimensions increases conduction, MIPT scientists discover July 6th, 2015

A Stretchy Mesh Heater for Sore Muscles July 6th, 2015

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project