Nanotechnology Now







Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > DNAsomes' can deliver multiple drugs or genetic therapy

DNAsomes begin with short chains of synthetic DNA designed to be complementary over part of their length so they will join into microscopic Y-shapes. A lipid molecule is attached, and fluorescent dyes can be attached for tracking. Drugs or RNA are chemically bonded to the Y-shaped unit, then many units assembled into a sphere, about the size of a virus, that can enter cells and deliver their payload.
DNAsomes begin with short chains of synthetic DNA designed to be complementary over part of their length so they will join into microscopic Y-shapes. A lipid molecule is attached, and fluorescent dyes can be attached for tracking. Drugs or RNA are chemically bonded to the Y-shaped unit, then many units assembled into a sphere, about the size of a virus, that can enter cells and deliver their payload.

Abstract:
DNA isn't just for genetics anymore. Cornell researchers are using synthetic DNA to make nanoparticles, dubbed DNAsomes, that can deliver drugs and genetic therapy to the insides of cells.

DNAsomes' can deliver multiple drugs or genetic therapy

Ithaca, NY | Posted on April 22nd, 2011

Dan Luo, professor of biological and environmental engineering, and colleagues report their work in the Jan. 3 issue of the journal Small.

DNAsomes, Luo said, can carry multiple drugs as well as RNA molecules designed to block the expression of genes, an improvement over other drug-delivery systems such as liposomes (tiny wrappers of the phospholipid molecules that make up cell membranes) or polymer nanoparticles. Also, some other delivery systems can be toxic to cells, the researchers said.

In its natural habitat in the nucleus of a cell, DNA consists of long chain molecules that are complementary, attaching to one another like a string of Lego blocks over their entire length to form the famous double helix. The Luo research group creates short chains of synthetic DNA designed to attach over only part of their length so they will join into shapes like crosses, Ts or Ys.

DNAsomes are assembled from Y-shaped units, each made up of three strands of DNA. A lipid molecule is attached to the tail of the Y, and drugs to be delivered are chemically bonded to the arms. When the goal is to block the expression of genes with molecules of siRNA (small interfering RNA), the synthetic DNA can be designed with a section complementary to the RNA so that the RNA will loosely attach to it. Delivering siRNA has been a particular challenge for other drug-delivery systems, the researchers noted.

In water solution, the combination of DNA, which is attracted to water molecules, and lipids, which are repelled by water, causes the Y units to self-assemble into hollow spheres from 100 to 5,000 nanometers in diameter, consisting of multiple layers of DNA, lipid and cargo.

"The beauty of this is that the body of the thing is also a body of drugs," Luo said. About the size of a virus, the DNAsome will be engulfed by the cell membrane and taken into a cell in a similar way as a virus, he explained. The DNAsome can be tagged with molecules that target a particular kind of cell, such as a cancer cell.

What happens inside the cell to release the drugs is still a "black box," Luo said, as it is with other drug-delivery systems, but tests show that the cargo is delivered. The researchers loaded DNAsomes with a fluorescent dye and introduced them into a culture of hamster ovarian cells. Microphotos showed the ovarian cells glowing under ultraviolet light. Notably, the researchers found, the cargo had been delivered both to the cytoplasm and nucleus of the cells, an important consideration for multiple drug delivery, since different drugs might have different targets in the cell. In later experiments the researchers verified the ability of DNAsomes to deliver multiple drugs as well as siRNA.

DNAsomes are named by analogy with liposomes. The suffix --some comes from a Latin word meaning "body."

The lead researcher on the study is Young Hoon Roh, who was a graduate student in the Luo group and has just received his Ph.D. David Muller, professor of applied and engineering physics, and his research group collaborated in the project. The research was supported by the U.S. Department of Agriculture and the National Science Foundation (NSF). One of the participating graduate students is supported by a Royal Thai Government Scholarship. Some of the work was performed at the Cornell Nanobiotechnology Center and the Cornell Center for Materials research, both supported by the NSF, Cornell and industrial affiliates.

####

For more information, please click here

Contacts:
Bill Steele
(607) 254-8093


Chronicle Online
312 College Ave.
Ithaca, NY 14850
607.255.4206


Blaine Friedlander
(607) 254-8093

Copyright © Cornell University

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Scientists join forces to reveal the mass and shape of single molecules April 27th, 2015

The 16th Trends in Nanotechnology International Conference (TNT 2015) unveils 25 Keynote Speakers: Call for abstracts open April 27th, 2015

Graphenea celebrates fifth anniversary April 27th, 2015

Sensor Designed in Iran Able to Remove Formaldehyde Gas from Environment April 27th, 2015

Govt.-Legislation/Regulation/Funding/Policy

Scientists join forces to reveal the mass and shape of single molecules April 27th, 2015

SEFCU, SUNY Poly CNSE Announce Winning Student-Led Teams in the 6th Annual $500,000 New York Business Plan Competition April 25th, 2015

Northwestern scientists develop first liquid nanolaser: Technology could lead to new way of doing 'lab on a chip' medical diagnostics April 25th, 2015

Electron spin brings order to high entropy alloys April 23rd, 2015

Nanomedicine

Scientists join forces to reveal the mass and shape of single molecules April 27th, 2015

Northwestern scientists develop first liquid nanolaser: Technology could lead to new way of doing 'lab on a chip' medical diagnostics April 25th, 2015

Nanotech-enabled moisturizer speeds healing of diabetic skin wounds: Spherical nucleic acids silence gene that interferes with wound healing April 24th, 2015

Fast and accurate 3-D imaging technique to track optically trapped particles April 24th, 2015

Discoveries

Scientists join forces to reveal the mass and shape of single molecules April 27th, 2015

Sensor Designed in Iran Able to Remove Formaldehyde Gas from Environment April 27th, 2015

Cacao Seed Extract Used in Production of Catalytic Nanoparticles April 27th, 2015

Heat makes electronsí spin in magnetic superconductors April 26th, 2015

Announcements

Scientists join forces to reveal the mass and shape of single molecules April 27th, 2015

The 16th Trends in Nanotechnology International Conference (TNT 2015) unveils 25 Keynote Speakers: Call for abstracts open April 27th, 2015

Graphenea celebrates fifth anniversary April 27th, 2015

Sensor Designed in Iran Able to Remove Formaldehyde Gas from Environment April 27th, 2015

Nanobiotechnology

Scientists join forces to reveal the mass and shape of single molecules April 27th, 2015

A silver lining: UCSB researchers cradle silver nanoclusters inside synthetic DNA to create a programmed, tunable fluorescent array April 23rd, 2015

Scientists Use Nanoscale Building Blocks and DNA 'Glue' to Shape 3D Superlattices: New approach to designing ordered composite materials for possible energy applications April 23rd, 2015

UCLA nanoscientists are first to model atomic structures of three bacterial nanomachines: Cryo electron microscope enables scientists to explore the frontiers of targeted antibiotics April 21st, 2015

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project