Nanotechnology Now

Our NanoNews Digest Sponsors



Heifer International

Wikipedia Affiliate Button


android tablet pc

Home > Press > With Chemical Modification, Stable RNA Nanoparticles Go 3-D

Peixuan Guo, PhD, Dane and Mary Louise Miller Endowed Chair in biomedical engineering with students in his lab at the Vontz Center for Molecular Studies
Peixuan Guo, PhD, Dane and Mary Louise Miller Endowed Chair in biomedical engineering with students in his lab at the Vontz Center for Molecular Studies

Abstract:
Researchers have found a way to bypass RNase and create stable three-dimensional configurations of RNA, greatly expanding the possibilities for RNA in nanotechnology (the engineering of functional systems at the molecular scale).

With Chemical Modification, Stable RNA Nanoparticles Go 3-D

Cincinnati, OH | Posted on January 24th, 2011

For years, RNA has seemed an elusive tool in nanotechnology research—easily manipulated into a variety of structures, yet susceptible to quick destruction when confronted with a commonly found enzyme.

"The enzyme RNase cuts RNA randomly into small pieces, very efficiently and within minutes," explains Peixuan Guo, PhD, Dane and Mary Louise Miller Endowed Chair and professor of biomedical engineering at the University of Cincinnati (UC). "Moreover, RNase is present everywhere, making the preparation of RNA in a lab extremely difficult."

But by replacing a chemical group in the macromolecule, Guo says he and fellow researchers have found a way to bypass RNase and create stable three-dimensional configurations of RNA, greatly expanding the possibilities for RNA in nanotechnology (the engineering of functional systems at the molecular scale).

Their results, "Fabrication of Stable and RNase-Resistant RNA Nanoparticles Active in Gearing the Nanomotors for Viral DNA Packaging," are published online in the journal ACS Nano.

In their work, Guo and his colleagues focused on the ribose rings that, together with alternating phosphate groups, form the backbone of RNA. By changing one section of the ribose ring, Guo and his team altered the structure of the molecule, making it unable to bind with RNase and able to resist degradation.

"RNase interaction with RNA requires a match of structural conformation," says Guo. "When RNA conformation has changed, the RNase cannot recognize RNA and the binding becomes an issue."

While he says previous researchers have shown this alteration makes RNA stable in a double helix, they did not study its potential to affect the folding of RNA into a three-dimensional structure necessary for nanotechnology.

After creating the RNA nanoparticle, Guo and his colleagues successfully used it to power the DNA packaging nanomotor of bacteriophage phi29, a virus that infects bacteria.

"We found that the modified RNA can fold into its 3-D structure appropriately, and can carry out its biological functions after modification," says Guo. "Our results demonstrate that it is practical to produce RNase-resistant, biologically active, and stable RNA for application in nanotechnology."

Because stable RNA molecules can be used to assemble a variety of nanostructures, Guo says they are an ideal tool to deliver targeted therapies to cancerous or viral-infected cells:

"RNA nanoparticles can be fabricated with a level of simplicity characteristic of DNA while possessing versatile structure and catalytic function similar to that of proteins. With this RNA modification, hopefully we can open new avenues of study in RNA nanotechnology."

Guo serves as director of UC's National Institutes of Health (NIH) Nanomedicine Development Center and Nanobiomedical Center. This work was funded by grants from the NIH.

Co-authors include Jing Liu, Mathieu Cinier and Yi Shu from UC, Chaoping Chen from Colorado State University, Guanxin Shen from Huazhong University of Science and Technology in China, and Songchuan Guo from Kylin Therapeutics, Inc.

####

For more information, please click here

Contacts:
Media Contact:
Katy Cosse
(513) 558-0207

Copyright © University of Cincinnati

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Piezoelectricity in a 2-D semiconductor: Berkeley Lab researchers discovery of piezoelectricty in molybdenum disulfide holds promise for future MEMS December 22nd, 2014

Quantum physics just got less complicated December 22nd, 2014

Enzyme Biosensor Used for Rapid Measurement of Drug December 22nd, 2014

Universality of charge order in cuprate superconductors: Charge order has been established in another class of cuprate superconductors, highlighting the importance of the phenomenon as a general property of these high-Tc materials December 22nd, 2014

Synthetic Biology

New tool could help reshape the limits of synthetic biology: The 'telomerator' reshapes synthetic yeast chromosome into more flexible, realistic form, redefining what geneticists can build November 3rd, 2014

Tiny carbon nanotube pores make big impact October 29th, 2014

Smallest world record has 'endless possibilities' for bio-nanotechnology October 8th, 2014

Artificial Cells Act Like the Real Thing: Cell-like compartments produce proteins and communicate with one another, similar to natural biological systems August 18th, 2014

Possible Futures

A novel method for identifying the body’s ‘noisiest’ networks November 19th, 2014

Researchers discern the shapes of high-order Brownian motions November 17th, 2014

VDMA Electronics Production Equipment: Growth track for 2014 and 2015 confirmed: Business climate survey shows robust industry sector November 14th, 2014

Open Materials Development Will Be Key for HP's Success in 3D Printing: HP can make a big splash in 3D printing, but it needs to shore up technology claims and avoid the temptation of the razor/razor blade business model in order to flourish November 11th, 2014

Academic/Education

SUNY Poly NanoCollege Faculty Member Selected as American Physical Society Fellow: SUNY Poly Associate Professor of Nanoscience Dr. Vincent LaBella Recognized for Significant Technological Innovations that Enable Interactive Learning December 17th, 2014

Nanomedicine expert joins Rice faculty: Gang Bao combines genetic, nano and imaging techniques to fight disease December 17th, 2014

FEI and Oregon Health & Science University Install a Complete Correlative Microscopy Workflow in Newly Built Collaborative Science Facility December 16th, 2014

Student Nanotechnology Laboratories Network Set Up in Iran December 15th, 2014

Nanomedicine

Enzyme Biosensor Used for Rapid Measurement of Drug December 22nd, 2014

Creation of 'Rocker' protein opens way for new smart molecules in medicine, other fields December 18th, 2014

Iranian Researchers Produce Electrical Pieces Usable in Human Body December 18th, 2014

Zenosense, Inc. - Hospital Collaboration - 400 Person Lung Cancer Detection Trial December 17th, 2014

Announcements

Piezoelectricity in a 2-D semiconductor: Berkeley Lab researchers discovery of piezoelectricty in molybdenum disulfide holds promise for future MEMS December 22nd, 2014

Quantum physics just got less complicated December 22nd, 2014

Enzyme Biosensor Used for Rapid Measurement of Drug December 22nd, 2014

Universality of charge order in cuprate superconductors: Charge order has been established in another class of cuprate superconductors, highlighting the importance of the phenomenon as a general property of these high-Tc materials December 22nd, 2014

Nanobiotechnology

Scientists trace nanoparticles from plants to caterpillars: Rice University study examines how nanoparticles behave in food chain December 16th, 2014

FEI and Oregon Health & Science University Install a Complete Correlative Microscopy Workflow in Newly Built Collaborative Science Facility December 16th, 2014

UCLA engineers first to detect and measure individual DNA molecules using smartphone microscope December 15th, 2014

Biomimetic dew harvesters: Understanding how a desert beetle harvests water from dew could improve drinking water collection in dew condensers December 8th, 2014

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2014 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE