Nanotechnology Now

Our NanoNews Digest Sponsors

Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Fitting a biological nanopore into a man-made one, new ways to analyze DNA

Artistic rendering of the formation of hybrid pores by the directed insertion of the biological protein pore alpha hemolysin (pink) into solid-state nanopores (holes in the green bottom layer). An applied electric field drives a double-stranded DNA molecule (blue, left) into the hole, which subsequently drags the pink hemolysin protein into position. Once assembled, these hybrid nanopores can be used to pull single-strand DNA (blue, center) through, for analysis and sequencing. Image courtesy Cees Dekker lab TU Delft / Tremani
Artistic rendering of the formation of hybrid pores by the directed insertion of the biological protein pore alpha hemolysin (pink) into solid-state nanopores (holes in the green bottom layer). An applied electric field drives a double-stranded DNA molecule (blue, left) into the hole, which subsequently drags the pink hemolysin protein into position. Once assembled, these hybrid nanopores can be used to pull single-strand DNA (blue, center) through, for analysis and sequencing. Image courtesy Cees Dekker lab TU Delft / Tremani

Abstract:
Researchers at Delft University of Technology and Oxford University announce a new type of nanopore device that could help in developing fast and cheap genetic analysis. In the journal Nature Nanotechnology (November 28), they report on a novel method that combines man-made and biological materials to result in a tiny hole on a chip, which is able to measure and analyze single DNA molecules.

Fitting a biological nanopore into a man-made one, new ways to analyze DNA

The Netherlands | Posted on November 29th, 2010

Biological

"The first mapping of the human genome - where the content of the human DNA was read off ('sequenced') - was completed in 2003 and it cost an estimated 3 billion US dollars. Imagine if that cost could drop to a level of a few 100 euro, where everyone could have their own personal genome sequenced. That would allow doctors to diagnose diseases and treat them before any symptoms arise." Professor Cees Dekker of the Kavli Institute of Nanoscience at Delft explains.

One promising device is called a nanopore: a minute hole that can be used to 'read' information from a single molecule of DNA as it threads through the hole.

New research by Dekker's group in collaboration with prof. Hagan Bayley of Oxford University, has now demonstrated a new, much more robust type of nanopore device. It combines biological and artificial building blocks.

Fragile

Dekker: 'Nanopores are already used for DNA analysis by inserting naturally occurring, pore-forming proteins into a liquid-like membrane made of lipids. DNA molecules can be pulled individually through the pore by applying an electrical voltage across it, and analyzed in much the same way that music is read from an old cassette tape as it is threaded through a player. One aspect that makes this biological technology especially difficult, however, is the reliance on the fragile lipid support layer. This new hybrid approach is much more robust and suitable to integrate nanopores into devices.'

Putting proteins onto a silicon chip

The new research, performed chiefly by lead author dr. Adam Hall, now demonstrates a simple method to implant the pore-forming proteins into a robust layer in a silicon chip. Essentially, an individual protein is attached to a larger piece of DNA, which is then pulled through a pre-made opening in a silicon nitride membrane. When the DNA molecule threads through the hole, it pulls the pore-forming protein behind it, eventually lodging it in the opening and creating a strong, chip-based system that is tailor-made for arrays and device applications. The researchers have shown that the hybrid device is fully functional and can be used to detect DNA molecules.

Article:
Title: Hybrid pore formation by directed insertion of alpha hemolysin into solid-state nanopores
Authors: Adam R. Hall1, Andrew Scott1, Dvir Rotem2, Kunal K. Mehta2, Hagan Bayley2, and Cees Dekker (*)

Address:
(*): Kavli Institute of Nanoscience, Delft University of Technology, Lorentzweg 1, 2628 CJ Delft, The Netherlands; 2: Department of Chemistry, University of Oxford, Chemistry Research Laboratory, Mansfield Road, OX1 3TA, Oxford, UK
Journal: Nature Nanotechnology. Advance Online Publication (AOP)

PDF: A pdf of the paper can be received upon request:

####

For more information, please click here

Contacts:
Cees Dekker

Copyright © Delft University of Technology

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

Composite Pipe Long Term Testing Facility February 10th, 2016

Scientists take nanoparticle snapshots February 10th, 2016

Chemical cages: New technique advances synthetic biology February 10th, 2016

New thin film transistor may lead to flexible devices: Researchers engineer an electronics first, opening door to flexible electronics February 10th, 2016

Possible Futures

Chemical cages: New technique advances synthetic biology February 10th, 2016

New thin film transistor may lead to flexible devices: Researchers engineer an electronics first, opening door to flexible electronics February 10th, 2016

Electron's 1-D metallic surface state observed: A step for the prediction of electronic properties of extremely-fine metal nanowires in next-generation semiconductors February 9th, 2016

A fast solidification process makes material crackle February 8th, 2016

Academic/Education

SUNY Poly and GLOBALFOUNDRIES Announce New $500M R&D Program in Albany To Accelerate Next Generation Chip Technology: Arrival of Second Cutting Edge EUV Lithography Tool Launches New Patterning Center That Will Generate Over 100 New High Tech Jobs at SUNY Poly February 9th, 2016

COD Grad Begins Postdoctoral Fellow at Harvard University: Marsela Jorgolli's Passion for Physics Has Led to a Decade of Academic Research That Continues at Harvard University as a Postdoctoral Fellow February 2nd, 2016

Heriot-Watt's Institute of Photonics & Quantum Sciences uses the Deben Microtest 2 kN tensile stage to characterise ceramics and engineering plastics January 21st, 2016

Multiple uses for the JPK NanoWizard AFM system in the Smart Interfaces in Environmental Nanotechnology Group at the University of Illinois at Urbana-Champaign January 20th, 2016

Nanomedicine

Nanoparticle therapy that uses LDL and fish oil kills liver cancer cells February 9th, 2016

Leading bugs to the death chamber: A kinder face of cholesterol February 8th, 2016

UTHealth research looks at nanotechnology to help prevent preterm birth February 7th, 2016

Scientists take key step toward custom-made nanoscale chemical factories: Berkeley Lab researchers part of team that creates new function in tiny protein shell structures February 6th, 2016

Announcements

Composite Pipe Long Term Testing Facility February 10th, 2016

Scientists take nanoparticle snapshots February 10th, 2016

Chemical cages: New technique advances synthetic biology February 10th, 2016

New thin film transistor may lead to flexible devices: Researchers engineer an electronics first, opening door to flexible electronics February 10th, 2016

Nanobiotechnology

Chemical cages: New technique advances synthetic biology February 10th, 2016

Nanoparticle therapy that uses LDL and fish oil kills liver cancer cells February 9th, 2016

Leading bugs to the death chamber: A kinder face of cholesterol February 8th, 2016

UTHealth research looks at nanotechnology to help prevent preterm birth February 7th, 2016

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More











ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







Car Brands
Buy website traffic