Nanotechnology Now







Heifer International

Wikipedia Affiliate Button


DHgate

Home > Press > Understanding How Cells Respond to Nanoparticles

Nanoparticles are finding utility in myriad biotechnological applications, including gene regulation, intracellular imaging, and medical diagnostics. Thus, evaluating the biocompatibility of these nanomaterials is imperative. Here we use genome-wide expression profiling to study the biological response of HeLa cells to gold nanoparticles functionalized with nucleic acids.
Nanoparticles are finding utility in myriad biotechnological applications, including gene regulation, intracellular imaging, and medical diagnostics. Thus, evaluating the biocompatibility of these nanomaterials is imperative. Here we use genome-wide expression profiling to study the biological response of HeLa cells to gold nanoparticles functionalized with nucleic acids.

Abstract:
Gold nanoparticles are showing real promise as vehicles for efficiently delivering therapeutic nucleic acids, such as disease-fighting genes and small interfering RNA (siRNA) molecules, to tumors. Now, a team of investigators from Northwestern University has shown that the safety of gold nanoparticle-nucleic acid formulations depends significantly on how the nucleic acids and nanoparticles are linked to one another, a finding with important implications for those researchers developing such constructs.

Understanding How Cells Respond to Nanoparticles

Bethesda, MD | Posted on October 27th, 2010

Chad Mirkin, co-principal investigator of the Northwestern University Center for Cancer Nanotechnology Excellence, one of nice such centers established by the National Cancer Institute (NCI), led the team of investigators that studied how cells respond to different nucleic acid-nanoparticle formulations. The investigators published their results in the journal ACS Nano.

To measure how cancer cells respond when they take up nanoparticles, Dr. Mirkin and his colleagues used a technique known as genome-wide expression profiling, which measures relative changes in global gene expression. The investigators added different types of nanoparticles to cancer cells growing in culture dishes and then obtained whole genome expression profiles for the cells. In all the experiments, the researchers attached non-targeting nucleic acids attached to the nanoparticles in order to minimize gene changes that might be triggered through a therapeutic effect relating to a specific, designed interaction between the nucleic acid and a targeted gene.

The results of these comparison studies showed that the surface properties of the nanoparticles had a profound impact on how a given nanoparticle impacts gene expression within a cell. The researchers observed the most surprising and noteworthy difference when they compared two nanoparticles that differed only in the manner in which the nucleic acids were attached to the nanoparticle surface. Nanoparticles loosely linked to the nucleic acids triggered large-scale changes in gene expression, while in contrast, nanoparticles linked tightly to nucleic acids through a covalent chemical bond had virtually no effect on gene expression. These findings, the researchers noted, show how important it is to fully characterize nanoparticles not only in terms of the shape and size, but also with respect to their surface properties.

This work, which is detailed in a paper titled, "Cellular Response of Polyvalent Oligonucleotide-Gold Nanoparticle Conjugates," was supported in part by the NCI Alliance for Nanotechnology in Cancer, a comprehensive initiative designed to accelerate the application of nanotechnology to the prevention, diagnosis, and treatment of cancer. An abstract of this paper is available at the journal's website.

View abstract at pubs.acs.org/doi/abs/10.1021/nn102228s

####

About NCI Alliance for Nanotechnology in Cancer
To help meet the goal of reducing the burden of cancer, the National Cancer Institute (NCI), part of the National Institutes of Health, is engaged in efforts to harness the power of nanotechnology to radically change the way we diagnose, treat and prevent cancer.

The NCI Alliance for Nanotechnology in Cancer is a comprehensive, systematized initiative encompassing the public and private sectors, designed to accelerate the application of the best capabilities of nanotechnology to cancer.

Currently, scientists are limited in their ability to turn promising molecular discoveries into benefits for cancer patients. Nanotechnology can provide the technical power and tools that will enable those developing new diagnostics, therapeutics, and preventives to keep pace with today’s explosion in knowledge.

For more information, please click here

Copyright © NCI Alliance for Nanotechnology in Cancer

If you have a comment, please Contact us.

Issuers of news releases, not 7th Wave, Inc. or Nanotechnology Now, are solely responsible for the accuracy of the content.

Bookmark:
Delicious Digg Newsvine Google Yahoo Reddit Magnoliacom Furl Facebook

Related News Press

News and information

'Atomic chicken-wire' is key to faster DNA sequencing March 30th, 2015

Nanoscale worms provide new route to nano-necklace structures March 29th, 2015

Solving molybdenum disulfide's 'thin' problem: Research team increases material's light emission by twelve times March 29th, 2015

A first glimpse inside a macroscopic quantum state March 28th, 2015

Govt.-Legislation/Regulation/Funding/Policy

Nanoscale worms provide new route to nano-necklace structures March 29th, 2015

UT Dallas engineers twist nanofibers to create structures tougher than bulletproof vests March 27th, 2015

Novel nanoparticle therapy promotes wound healing March 27th, 2015

Designer's toolkit for dynamic DNA nanomachines: Arm-waving nanorobot signals new flexibility in DNA origami March 27th, 2015

Academic/Education

LAMDAMAP 2015 hosted by the University March 26th, 2015

SUNY Poly & M+W Make Major Announcement: Major Expansion To Include M+W Owned Gehrlicher Solar America Corporation That Will Create up to 400 Jobs to Develop Solar Power Plants at SUNY Poly Sites Across New York State March 26th, 2015

SUNY POLY CNSE to Host First Ever Northeast Semi Supply Conference (NESCO) Conference Will Connect New and Emerging Innovators in the Northeastern US and Canada with Industry Leaders and Strategic Investors to Discuss Future Growth Opportunities in NYS March 25th, 2015

FEI Joins University of Ulm and CEOS on SALVE Project Research Collaboration: The Sub-Ångström Low Voltage Electron (SALVE) microscope should improve contrast and reduce damage on bio-molecules and two-dimensional nanomaterials, such as graphene March 18th, 2015

Nanomedicine

'Atomic chicken-wire' is key to faster DNA sequencing March 30th, 2015

Novel nanoparticle therapy promotes wound healing March 27th, 2015

Graphene reduces wear of alumina ceramic March 26th, 2015

Application of Graphene Oxide in Body Implants in Iran March 26th, 2015

Announcements

'Atomic chicken-wire' is key to faster DNA sequencing March 30th, 2015

Nanoscale worms provide new route to nano-necklace structures March 29th, 2015

Solving molybdenum disulfide's 'thin' problem: Research team increases material's light emission by twelve times March 29th, 2015

A first glimpse inside a macroscopic quantum state March 28th, 2015

Nanobiotechnology

'Atomic chicken-wire' is key to faster DNA sequencing March 30th, 2015

Designer's toolkit for dynamic DNA nanomachines: Arm-waving nanorobot signals new flexibility in DNA origami March 27th, 2015

Dolomite’s microfluidics technology ideal for B cell encapsulation March 24th, 2015

Tiny bio-robot is a germ suited-up with graphene quantum dots March 24th, 2015

NanoNews-Digest
The latest news from around the world, FREE




  Premium Products
NanoNews-Custom
Only the news you want to read!
 Learn More
NanoTech-Transfer
University Technology Transfer & Patents
 Learn More
NanoStrategies
Full-service, expert consulting
 Learn More










ASP
Nanotechnology Now Featured Books




NNN

The Hunger Project







© Copyright 1999-2015 7th Wave, Inc. All Rights Reserved PRIVACY POLICY :: CONTACT US :: STATS :: SITE MAP :: ADVERTISE